Full Text:   <2762>

CLC number: Q949.32

On-line Access: 

Received: 2005-08-18

Revision Accepted: 2005-10-21

Crosschecked: 0000-00-00

Cited: 2

Clicked: 5431

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.1 P.28-33


Promoter trapping in Magnaporthe grisea

Author(s):  Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng

Affiliation(s):  Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   minhang@zju.edu.cn, fuchenglin@zju.edu.cn

Key Words:  Promoter trapping, Green fluorescent protein, Magnaporthe grisea

Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng. Promoter trapping in Magnaporthe grisea[J]. Journal of Zhejiang University Science B, 2006, 7(1): 28-33.

@article{title="Promoter trapping in Magnaporthe grisea",
author="Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Promoter trapping in Magnaporthe grisea
%A Liu Xiao-hong
%A Lu Jian-ping
%A Wang Jiao-yu
%A Min Hang
%A Lin Fu-cheng
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 1
%P 28-33
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0028

T1 - Promoter trapping in Magnaporthe grisea
A1 - Liu Xiao-hong
A1 - Lu Jian-ping
A1 - Wang Jiao-yu
A1 - Min Hang
A1 - Lin Fu-cheng
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 1
SP - 28
EP - 33
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0028

Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Balhadère, P.V., Foster, A.J., Talbot, N.J., 1999. Identification of pathogenicity mutants of the rice blast fungus Magnaporthe grisea using insertional mutagenesis. Mol. Plant–Microbe Interact., 12:129-142.

[2] Bellen, H.J., 1999. Ten years of enhancer detection: lessons from the fly. Plant Cell, 11:2271-2281.

[3] Carroll, A.M., Sweigard, J.A., Valent, B., 1994. Improved vectors for selecting resistance to hygromycin. Fungal Genet Newsl, 41:22.

[4] Chang, W.T., Gross, J.D., Newell, P.C., 1995. Trapping developmental promoters in Dictyostelium. Plasmid, 34:175-183.

[5] Dean, R.A., 1997. Signal pathways and appressorium morphogenesis. Annu. Rev. Phytopatho., 35:211-234.

[6] Fobert, P.R., Miki, B.L., Iyer, V.N., 1991. Detection of gene regulatory signals in plants revealed by T-DNA-mediated fusions. Plant Mol. Biol., 17:837-851.

[7] Gossler, A., Joyner, A.L., Rossant, J., Skarnes, W.C., 1989. Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science, 244:463-465.

[8] Irie, T., Matsumura, H., Terauchi, R., Saitoh, H., 2003. Serial analysis of gene expression (SAGE) of Magnaporthe grisea: genes involved in appressorium formation. Mol. Genet. Genomics, 270:181-189.

[9] Kamakura, T., Xiao, J.Z., Choi, W.B., Kochi, T., Yamaguchi, S., Teraoka, T., Yamaguchi, I., 1999. cDNA subtractive cloning of genes expressed during early stage of appressorium formation by Magnaporthe grisea. Biosci. Biotechnol. Biochem., 63:1407-1413.

[10] Lu, J.P., Liu, T.B., Yu, X.Y., Lin, F.C., 2005. Representative appressorium stage cDNA library of Magnaporthe grisea. J. Zhejiang Univ. Sci., 6B(2):132-136.

[11] Mullins, E.D., Kang, S., 2001. Transformation: a tool for studying fungal pathogens of plants. Cell. Mol. Life Sci., 58:2043-2052.

[12] Rauyaree, R., Choi, W., Fang, E., Blackmon, B., Dean, R.A., 2001. Genes expressed during early stages of rice infection with the rice blast fungus Magnaporthe grisea. Mol. Plant Pathol., 2:347-354.

[13] Retallack, D.M., Deepe, G.S., Woods, J.P., 2000. Applying in vivo expression technology (IVET) to the fungal pathogen Histoplasma capsulatum. Microbial Pathogenesis, 28:169-182.

[14] Rho, H.S., Kang, S., Lee, Y.H., 2001. Agrobacterium tumefaciens-mediated transformation of the plant pathogenic fungus, Magnaporthe grisea. Mol. Cells, 12(3):407-411.

[15] Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

[16] Springer, P.S., 2000. Gene traps: tools for plant development and genomics. Plant Cell, 12:1007-1020.

[17] Takano, Y., Choi, W., Mitchell, T.K., Okuno, T., Dean, R.A., 2003. Large scale parallel analysis of gene expression during infection-related morphogenesis of Magnaporthe grisea. Mol. Plant Pathol., 4:337-347.

[18] Talbot, N.J., 2003. On the trail of a cereal killer: exploring the biology of Magnaporthe grisea. Annual Review of Microbiology., 57(1):177-202.

[19] Talbot, N.J., Ebbole, D.J., Hamer, J.E., 1993. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell, 5:1575-1590.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE