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Received: 2006-07-03
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Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions
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VASHIST Sandeep Kumar, KAUR Inderpreet, BAJPAI Ram Prakash, BHARADWAJ Lalit Mohan, TEWARI Rupinder, RAITERI Roberto. Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions[J]. Journal of Zhejiang University Science B, 2006, 7(9): 683-685.
@article{title="Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions",
author="VASHIST Sandeep Kumar, KAUR Inderpreet, BAJPAI Ram Prakash, BHARADWAJ Lalit Mohan, TEWARI Rupinder, RAITERI Roberto",
journal="Journal of Zhejiang University Science B",
volume="7",
number="9",
pages="683-685",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0683"
}
%0 Journal Article
%T Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions
%A VASHIST Sandeep Kumar
%A KAUR Inderpreet
%A BAJPAI Ram Prakash
%A BHARADWAJ Lalit Mohan
%A TEWARI Rupinder
%A RAITERI Roberto
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 9
%P 683-685
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0683
TY - JOUR
T1 - Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions
A1 - VASHIST Sandeep Kumar
A1 - KAUR Inderpreet
A1 - BAJPAI Ram Prakash
A1 - BHARADWAJ Lalit Mohan
A1 - TEWARI Rupinder
A1 - RAITERI Roberto
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 9
SP - 683
EP - 685
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0683
Abstract: We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been observed for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass substrate changed from 1.02×10−8 Ω−1 to 1.41×10−11 Ω−1 at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab′)2. Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21 polyclonal antibodies interacting with p21 antigen. The proposed concept can represent a new immunodiagnostic technique and may have wide ranging applications in biosensors and nanobiotechnology too.
[1] Long, Y.T., Abu-Irhayem, E., Kraatz, H.B., 2005. Peptide electron transfer: more questions than answers. Chem. Eur. J., 11(18):5186-5194.
[2] Schlag, E.W., Sheu, S.Y., Yang, D.Y., Selzle, H.L., Lin, S.H., 2000a. Charge conductivity in peptides: dynamic simulations of a bifunctional model supporting experimental data. Proc. Natl. Acad. Sci. USA, 97(3):1068-1072.
[3] Schlag, E.W., Yang, D.Y., Sheu, S.Y., Selzle, H.L., Lin, S.H., Rentzepis, P.M., 2000b. Dynamical principles in biological processes: a model of charge migration in proteins and DNA. Proc. Natl. Acad. Sci. USA, 97(18):9849-9854.
[4] Sheu, S.Y., Schlag, E.W., 2002. Protein charge transport in gas phase. Int. J. Mass Spectrometry, 219(1):73-77.
[5] Sheu, S.Y., Yang, D.Y., Selzle, H.L., Schlag, E.W., 2002. Charge transport in a polypeptide chain. Eur. Phys. J. D, 20(3):557-563.
[6] Weinkauf, R., Schanen, P., Yang, D., Soukara, S., Schlag, E.W., 1995. Elementary processes in peptides: electron mobility and dissociation in peptide cations in the gas phase. J. Phys. Chem., 99(28):11255-11265.
[7] Weinkauf, R., Schanen, P., Metsala, A., Schlag, E.W., Burgle, M., Kessler, H., 1996. Highly efficient charge transfer in peptide cations in the gas phase―Threshold effects and mechanism. J. Phys. Chem., 100(47):18567-18585.
[8] Weinkauf, R., Schlag, E.W., Martinez, T.J., Levine, R.D., 1997. Nonstationary electronic states and site-selective reactivity. J. Phys. Chem. A, 101(42):7702-7710.
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