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On-line Access: 2018-11-02

Received: 2017-10-10

Revision Accepted: 2018-07-12

Crosschecked: 2018-10-10

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Yue-qin Qiu


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.11 P.884-894


A high-fat diet increases body fat mass and up-regulates expression of genes related to adipogenesis and inflammation in a genetically lean pig

Author(s):  Xue-fen Yang, Yue-qin Qiu, Li Wang, Kai-guo Gao, Zong-yong Jiang

Affiliation(s):  Laboratory of Animal Nutrition and Feed (South China), Ministry of Agriculture / State Key Laboratory of Livestock and Poultry Breeding / Guangdong Key Laboratory of Animal Breeding and Nutrition / Guangdong Public Laboratory of Animal Breeding and Nutrition / Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Corresponding email(s):   jiangz28@qq.com

Key Words:  Genetically lean pig, Diet-induced obesity, High-fat diet, Adiposity deposition, Microarray analysis, Inflammation

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Xue-fen Yang, Yue-qin Qiu, Li Wang, Kai-guo Gao, Zong-yong Jiang. A high-fat diet increases body fat mass and up-regulates expression of genes related to adipogenesis and inflammation in a genetically lean pig[J]. Journal of Zhejiang University Science B, 2018, 19(11): 884-894.

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%T A high-fat diet increases body fat mass and up-regulates expression of genes related to adipogenesis and inflammation in a genetically lean pig
%A Xue-fen Yang
%A Yue-qin Qiu
%A Li Wang
%A Kai-guo Gao
%A Zong-yong Jiang
%J Journal of Zhejiang University SCIENCE B
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%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700507

T1 - A high-fat diet increases body fat mass and up-regulates expression of genes related to adipogenesis and inflammation in a genetically lean pig
A1 - Xue-fen Yang
A1 - Yue-qin Qiu
A1 - Li Wang
A1 - Kai-guo Gao
A1 - Zong-yong Jiang
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1700507

Because of their physiological similarity to humans, pigs provide an excellent model for the study of obesity. This study evaluated diet-induced adiposity in genetically lean pigs and found that body weight and energy intake did not differ between controls and pigs fed the high-fat (HF) diet for three months. However, fat mass percentage, adipocyte size, concentrations of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), insulin, and leptin in plasma were significantly higher in HF pigs than in controls. The HF diet increased the expression in backfat tissue of genes responsible for cholesterol synthesis such as Insig-1 and Insig-2. Lipid metabolism-related genes including sterol regulatory element binding protein 1c (SREBP-1c), fatty acid synthase 1 (FASN1), diacylglycerol O-acyltransferase 2 (DGAT2), and fatty acid binding protein 4 (FABP4) were significantly up-regulated in backfat tissue, while the expression of proliferator-activated receptor-α (PPAR-α) and carnitine palmitoyl transferase 2 (CPT2), both involved in fatty acid oxidation, was reduced. In liver tissue, HF feeding significantly elevated the expression of SREBP-1c, FASN1, DGAT2, and hepatocyte nuclear factor-4α (HNF-4α) mRNAs. microarray analysis further showed that the HF diet had a significant effect on the expression of 576 genes. Among these, 108 genes were related to 21 pathways, with 20 genes involved in adiposity deposition and 26 related to immune response. Our results suggest that an HF diet can induce genetically lean pigs into obesity with body fat mass expansion and adipose-related inflammation.




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[34]List of electronic supplementary materials

[35]Fig. S1 Gene ontology (GO) analyses showing the significant differentially expressed genes

[36]Table S1 Significant pathways of up-regulated genes

[37]Table S2 Significant pathways of down-regulated genes

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