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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.5 P.385-392


Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis

Author(s):  Jin-long Cui, Shun-xing Guo, Pei-gen Xiao

Affiliation(s):  Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China

Corresponding email(s):   sxguo2006@yahoo.com.cn

Key Words:  Agar diffusion method, Agarwood, Antimicrobial bioactivity, Antitumor bioactivity, Endophytic fungi

Jin-long Cui, Shun-xing Guo, Pei-gen Xiao. Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis[J]. Journal of Zhejiang University Science B, 2011, 12(5): 385-392.

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author="Jin-long Cui, Shun-xing Guo, Pei-gen Xiao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis
%A Jin-long Cui
%A Shun-xing Guo
%A Pei-gen Xiao
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000330

T1 - Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis
A1 - Jin-long Cui
A1 - Shun-xing Guo
A1 - Pei-gen Xiao
J0 - Journal of Zhejiang University Science B
VL - 12
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SP - 385
EP - 392
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Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000330

The purpose of this study was to isolate and characterize endophytic fungi from the stem tissue which can produce fragrant ingredients in Aquilaria sinensis (also called agarwood) to determine their antitumor and antimicrobial activities. Twenty-eight fungal endophytes were isolated from agarwood by strict sterile sample preparation and were classified into 14 genera and 4 taxonomic classes (Sordariomycetes, Dothideomycetes, Saccharomycetes, and Zygomycetes) based on molecular identification. Of the 28 isolates, 13 (46.4%) showed antimicrobial activity against at least one of the test strains by the agar well diffusion method, and 23 isolates (82.1%) displayed antitumor activity against at least one of five cancer cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The diameters of inhibition zones of YNAS07, YNAS14, HNAS04, HNAS05, HNAS08, and HNAS11 were equal to or higher than 14.0 mm against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, B. subtilis, Aspergillus fumigatus, and B. subtilis, respectively. The inhibition rates of YNAS06, YNAS08, and HNAS06 were not less than 60% to 293-T, 293-T, and SKVO3 cells, respectively. These results suggest that the endophytic fungi associated with agarwood will provide us with not only useful micro-ecological information, but also potential antimicrobial and antitumor agents.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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