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CLC number: Q89

On-line Access: 2014-06-03

Received: 2014-04-03

Revision Accepted: 2014-07-23

Crosschecked: 2014-08-15

Cited: 7

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.9 P.766-775


Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo*

Author(s):  Jian Yin1, Jian-ming Yang1, Feng Zhang2, Peng Miao1,3, Ying Lin1, Ming-li Chen1

Affiliation(s):  1. CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academic of Sciences, Suzhou 215163, China; more

Corresponding email(s):   yinj@sibet.ac.cn

Key Words:  Joint toxicity, Cadmium sulfate, &alpha, -Naphthoflavone, Zebrafish embryo, Oxidative stress

Jian Yin, Jian-ming Yang, Feng Zhang, Peng Miao, Ying Lin, Ming-li Chen. Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo[J]. Journal of Zhejiang University Science B, 2014, 15(9): 766-775.

@article{title="Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo",
author="Jian Yin, Jian-ming Yang, Feng Zhang, Peng Miao, Ying Lin, Ming-li Chen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo
%A Jian Yin
%A Jian-ming Yang
%A Feng Zhang
%A Peng Miao
%A Ying Lin
%A Ming-li Chen
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 9
%P 766-775
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400091

T1 - Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo
A1 - Jian Yin
A1 - Jian-ming Yang
A1 - Feng Zhang
A1 - Peng Miao
A1 - Ying Lin
A1 - Ming-li Chen
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 9
SP - 766
EP - 775
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400091

This paper aims to evaluate the individual and joint toxicities of cadmium sulfate (CdSO4) and &alpha;-Naphthoflavone (ANF) in zebrafish embryos. As a result, CdSO4 caused both lethal and sub-lethal effects, such as 24 h post-fertilization (hpf) death and 72 hpf delayed hatching. However, ANF only caused sub-lethal effects, including 48 hpf cardiac edema and 72 hpf delayed hatching. Taking 24 hpf death and 48 hpf cardiac edema as endpoints, the toxicities of CdSO4 and ANF were significantly enhanced by each other. Consistently, both CdSO4 and ANF caused significant oxidative stress, including decreases in the reduced glutathione (GSH) level, inhibition of superoxide dismutase (SOD) activity, as well as increases in malondialdehyde (MDA) content in zebrafish embryos, but these mixtures produced much more significant alterations on the biomarkers. Co-treatment of CdSO4 and ANF significantly down-regulated the mRNA level of multidrug resistance-associated protein (mrp) 1 and cytochrome P450 (cyp) 1a, which constituted the protective mechanisms for zebrafish embryos to chemical toxins. In conclusion, co-treatment of CdSO4 and ANF exhibited a much more severe damage in zebrafish embryos than individual treatment. Meanwhile, production of oxidative stress and altered expression of mrp1 and cyp1a could be important components of such joint toxicity.



Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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