Full Text:   <1234>

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CLC number: X131

On-line Access: 2018-05-05

Received: 2018-01-18

Revision Accepted: 2018-03-20

Crosschecked: 2018-04-18

Cited: 0

Clicked: 2545

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hong-ting Zhao

https://orcid.org/0000-0002-4562-4576

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.5 P.400-408

http://doi.org/10.1631/jzus.B1800033


Neurological responses of embryo-larval zebrafish to short-term sediment exposure to decabromodiphenylethane


Author(s):  Mei-qing Jin, Dong Zhang, Ying Zhang, Shan-shan Zhou, Xian-ting Lu, Hong-ting Zhao

Affiliation(s):  College of Materials Science and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China; more

Corresponding email(s):   info-iem@hdu.edu.cn

Key Words:  Decabromodiphenylethane, Flame retardant, Danio rerio, Neurotoxicity


Mei-qing Jin, Dong Zhang, Ying Zhang, Shan-shan Zhou, Xian-ting Lu, Hong-ting Zhao. Neurological responses of embryo-larval zebrafish to short-term sediment exposure to decabromodiphenylethane[J]. Journal of Zhejiang University Science B, 2018, 19(5): 400-408.

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author="Mei-qing Jin, Dong Zhang, Ying Zhang, Shan-shan Zhou, Xian-ting Lu, Hong-ting Zhao",
journal="Journal of Zhejiang University Science B",
volume="19",
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pages="400-408",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800033"
}

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%T Neurological responses of embryo-larval zebrafish to short-term sediment exposure to decabromodiphenylethane
%A Mei-qing Jin
%A Dong Zhang
%A Ying Zhang
%A Shan-shan Zhou
%A Xian-ting Lu
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A1 - Dong Zhang
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A1 - Shan-shan Zhou
A1 - Xian-ting Lu
A1 - Hong-ting Zhao
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1800033


Abstract: 
decabromodiphenylethane (DBDPE) has been widely used as an alternative flame retardant due to the restriction or phase-out of traditional polybrominated diphenyl ethers (PBDEs), and is of increasing concern regarding its ubiquity, persistence, and potential adverse effects. In the present study, the toxicological effects of DBDPE were evaluated using zebrafish as an in vivo model. Upon being exposed to DBDPE-polluted sediments for a short term, it was found that the mortality and malformation of zebrafish (including edema, bent notochord, and bent tail) were not affected even at the highest concentration tested (1000.0 µg/kg dry sediment). Regarding behavioral responses, it was found that zebrafish larvae of 48 hours post fertilization (hpf) in all groups escaped successfully with a touch to the dorsal fin. However, when exposed to the highest DBDPE concentration, the larvae of 120 hpf exhibited significantly smaller distances as compared to the control. Moreover, the results of the acetylcholinesterase (AChE) activity, the expression levels of two important nerve-related genes, and the cell apoptosis all indicated that DBDPE posed low neurotoxicity in embryo-larval zebrafish. The results in this study shed some light on the potential risks of DBDPE in the real environment and highlight the application of the sediment exposure route in the future.

底泥中的十溴二苯乙烷的短期暴露对斑马鱼胚胎和幼鱼神经毒性的研究

目的:评估底泥中的十溴二苯乙烷(DBDPE)对斑马鱼早期发育阶段的胚胎毒性和神经行为毒性,并探索其潜在影响机制.
创新点:底泥暴露能更真实地反应DBDPE等强疏水性污染物在实际环境中的暴露情况,有利于污染物生态风险评估的科学性和准确性.
方法:将受精后4小时(4 hpf)的斑马鱼胚胎置于对照底泥和染毒底泥(DBDPE系列浓度)中进行短期暴露,观察不同发育阶段的存活率、孵化率、畸形率以及行为(包括触碰反应和自由泳动)效应;并通过斑马鱼幼鱼的乙酰胆碱酶活性、神经系统的相关基因(α1-tubulingap43)的转录水平以及斑马鱼整体组织的细胞凋亡情况的检测探讨其神经毒性的潜在机制.
结论:DBDPE从4 hpf处理至120 hpf,各浓度组的斑马鱼均未出现明显的畸形和死亡.在72 hpf时,最低浓度组(62.5 µg/kg)DBDPE轻微加快了斑马鱼的孵化,而最高浓度组(1000.0 µg/kg)DBDPE轻微延迟斑马鱼的孵化.所有浓度组的DBDPE对48 hpf时斑马鱼的触碰反应没有任何影响,最高浓度组(1000.0 µg/kg)DBDPE对120 hpf时斑马鱼的自由泳动总距离有显著的抑制作用(P<0.05).但是,斑马鱼的乙酰胆碱酶活性、α1-tubulingap43的转录水平未发生显著变化,所有浓度组的DBDPE亦均未诱发斑马鱼整体组织的细胞凋亡.

关键词:十溴二苯乙烷;阻燃剂;斑马鱼;神经毒性

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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