Full Text:   <1934>

Summary:  <1492>

CLC number: Q968

On-line Access: 2015-04-03

Received: 2014-06-01

Revision Accepted: 2014-12-10

Crosschecked: 2015-03-17

Cited: 0

Clicked: 3989

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Fa-zhong Yang

http://orcid.org/0000-0002-3962-6938

Chun Xiao

http://orcid.org/0000-0002-7245-4509

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.4 P.264-274

http://doi.org/10.1631/jzus.B1400151


Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid


Author(s):  Fa-zhong Yang, Bin Yang, Bei-bei Li, Chun Xiao

Affiliation(s):  School of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; more

Corresponding email(s):   x.chun@163.com

Key Words:  Toxin, Induced resistance, Fungus-plant-insect system, Plant-mediated interaction, Tenuazonic acid, Alternaria alternata, Macrosiphum rosivorum


Fa-zhong Yang, Bin Yang, Bei-bei Li, Chun Xiao. Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid[J]. Journal of Zhejiang University Science B, 2015, 16(4): 264-274.

@article{title="Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid",
author="Fa-zhong Yang, Bin Yang, Bei-bei Li, Chun Xiao",
journal="Journal of Zhejiang University Science B",
volume="16",
number="4",
pages="264-274",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400151"
}

%0 Journal Article
%T Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid
%A Fa-zhong Yang
%A Bin Yang
%A Bei-bei Li
%A Chun Xiao
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 4
%P 264-274
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400151

TY - JOUR
T1 - Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid
A1 - Fa-zhong Yang
A1 - Bin Yang
A1 - Bei-bei Li
A1 - Chun Xiao
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 4
SP - 264
EP - 274
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400151


Abstract: 
Many different types of toxins are produced by the fungus, Alternaria alternata (Fr.) Keissler. Little is known, however, regarding the influence of these toxins on insects. In this study, we investigated the toxin-induced inhibitory effects of the toxin produced by A. alternata on the rose aphid, Macrosiphum rosivorum, when the toxin was applied to leaves of the rose, Rosa chinensis. The results demonstrated that the purified crude toxin was non-harmful to rose plants and rose aphids, but had an intensive inhibitory effect on the multiplication of aphids. The inhibitory index against rose aphids reached 87.99% when rose plants were sprayed with the toxin solution at a low concentration. Further results from bioassays with aphids and high performance liquid chromatography (HPLC) analyses demonstrated that tenuazonic acid (TeA) was one of the most important resistance-related active components in the crude toxin. The content of TeA was 0.1199% in the crude toxin under the HPLC method. Similar to the crude toxin, the inhibitory index of pure TeA reached 83.60% 15 d after the rose plants were sprayed with pure TeA solution at the lower concentration of 0.060 μg/ml, while the contents of residual TeA on the surface and in the inner portion of the rose plants were only 0.04 and 0.00 ng/g fresh weight of TeA-treated rose twigs, respectively, 7 d after the treatment. Our results show that TeA, an active component in the A. alternata toxin, can induce the indirect plant-mediated responses in rose plants to intensively enhance the plant’s resistances against rose aphids, and the results are very helpful to understand the plant-mediated interaction between fungi and insects on their shared host plants.

链格孢菌毒素能诱导中国月季产生抗蚜活性的细交链孢菌酮酸

中文概要:
目的:研究链格孢菌毒素能诱导中国月季植株产生对月季长管蚜的抗性,从而证实寄主植物介导的病虫互作关系的存在,并研究其互作机制。
创新点:证实了一种对寄主植物和害虫均无毒性的真菌毒素能使寄主植物产生对昆虫的诱导抗性。
方法:马铃薯葡萄糖琼脂(PDA)培养基培养链格孢菌获得毒素粗品,大孔树脂纯化后配制成不同浓度的溶液,喷施到中国月季植株上。处理结束后接种月季长管蚜,与对照相比,计算毒素处理对蚜虫的抑制百分数。用高效液相色谱法(HPLC)结合标准品分析毒素中是否存在细交链孢菌酮酸(TeA),并测定其含量。TeA同法处理中国月季植株,测定TeA对蚜虫的抑制率,并与毒素粗品比较。再通过HPLC法测定植物体表和体内残留的TeA,以证明TeA能自然降解完全。
结论:(1)链格孢菌粗毒素和对照品TeA均能使中国月季植株产生对月季长管蚜的系统诱导抗性,显 著降低月季长管蚜对中国月季的危害;(2)链格孢菌粗毒素中的主要抗蚜活性成分是TeA,TeA有望成为中国月季上具有抗蚜活性的先导化合物;(3)粗毒素和对照品TeA对蚜虫和植物均无伤害作用,但能激活植物对虫害的诱导抗性(ISR)和系统获得性抗性(SAR),可直接证实二者间存在着寄主植物介导的间接的病虫互作关系。

关键词:链格孢菌毒素;细交链孢菌酮酸;病虫互作关系;高效液相色谱法(HPLC);诱导抗性

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Abbas, H.K., Vesonder, R.F., Boyette, C.D., et al., 1993. Phytotoxicity of AAL-toxin and other compounds produced by Alternaria alternata to jimsonweed (Datura stramonium). Can. J. Bot., 71(1):155-160.

[2]Berestetskiy, A.O., 2008. A review of fungal phytotoxins: from basic studies to practical use. Appl. Biochem. Microbiol., 44(5):453-465.

[3]Bostock, R.M., Karban, R., Thaler, J.S., et al., 2001. Signal interactions in induced resistance to pathogens and insect herbivores. Eur. J. Plant Pathol., 107(1):103-111.

[4]Chelkowski, J., Visconti, A., 1992. Alternaria: Biology, Plant Diseases and Metabolites. Elsevier, Amsterdam, the Netherlands, p.449-541.

[5]Chen, S., Dai, X., Qiang, S., et al., 2005. Effect of a nonhost-selective toxin from Alternaria alternata on chloroplast-electron transfer activity in Eupatorium adenophorum. Plant Pathol., 54(5):671-677.

[6]Cipollini, D., Enright, S., Traw, M.B., et al., 2004. Salicylic acid inhibits jasmonic acid-induced resistance of Arabidopsis thaliana to Spodoptera exigua. Mol. Ecol., 13(6):1643-1653.

[7]Davis, N.D., Diener, U.L., Morgan-Jones, G., 1977. Tenuazonic acid production by Alternaria alternata and Alternaria tenuissima isolated from cotton. Appl. Environ. Microbiol., 34(2):155-157.

[8]Griffin, G.F., Chu, F.S., 1983. Toxicity of the Alternaria metabolites alternariol, alternariol methyl ether, altenuene, and tenuazonic acid in the chicken embryo assay. Appl. Environ. Microbiol., 46(6):1420-1422.

[9]Hammerschmidt, R., 1999. Induced disease resistance: how do induced plants stop pathogens? Physiol. Mol. Plant Pathol., 55(2):77-84.

[10]Hatcher, P.E., Paul, N.D., Ayres, P.G., et al., 1995. Interactions between Rumex spp., herbivore and a rust fungus: the effect of Uromyces rumicis infection on leaf nutritional quality. Funct. Ecol., 9(1):97-105.

[11]Heath, M.C., Skalamera, D., 1997. Cellular interactions between plants and biotrophic fungal parasites. Adv. Bot. Res., 24(6):195-225.

[12]Heil, M., Bostock, R., 2002. Induced systemic resistance (ISR) against pathogens in the context of induced plant defences. Ann. Bot., 89(5):503-512.

[13]Hunter, M.D., 2000. Mixed signals and cross-talk: interactions between plants, insects and plant pathogens. Agric. For. Entomol., 2(3):155-161.

[14]Kaul, S., Wani, M., Dhar, K.L., et al., 2008. Production and GC-MS trace analysis of methyl eugenol from endophytic isolate of Alternaria from rose. Ann. Microbiol., 58(3):443-445.

[15]Kloepper, J.W., Tuzun, S., Kuć, J.A., 1992. Proposed definitions related to induced disease resistance. Biocontrol Sci. Technol., 2(4):349-351.

[16]Kruess, L., 2002. Indirect interaction between a fungal plant pathogen and a herbivorous beetle of the weed Cirsium arvense. Oecologia, 130(4):563-569.

[17]Ma, Y.F., Xiao, C., 2013. Push-pull effects of three plant secondary metabolites on oviposition of the potato tuber moth, Phthorimaea operculella. J. Insect Sci., 13:128.

[18]Montesano, M., Brader, G., Palva, E.T., 2003. Pathogen derived elicitors: searching for receptors in plants. Mol. Plant Pathol., 4(1):73-79.

[19]Ostry, V., 2008. Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs. World Mycotoxin J., 1(2):175-188.

[20]Paul, N.D., Hatcher, P.E., Taylor, J.E., 2000. Coping with multiple enemies: an integration of molecular and ecological perspectives. Trends Plant Sci., 5(5):220-225.

[21]Rayamajhi, M.B., Van, T.K., Pratt, P.D., et al., 2006. Interactive association between Puccinia psidii and Oxyops vitiosa, two introduced natural enemies of Melaleuca quinquenervia in Florida. Biol. Control, 37(1):56-67.

[22]Rosett, T., Sankhala, R.H., Stickings, C.E., et al., 1957. Studies in the biochemistry of micro-organism. 103. Metabolites of Alternaria tenuis Auct.: culture filtrate products. Biochem. J., 67(3):390-400.

[23]Rostás, M., Hilker, M., 2002. Asymmetric plant-mediated cross-effects between a herbivorous insect and a phytopathogenic fungus. Agric. Forest Entomol., 4(3):223-231.

[24]Rostás, M., Simon, M., Hilker, M., 2003. Ecological cross-effects of induced plant responses towards herbivores and phytopathogenic fungi. Basic Appl. Ecol., 4(1):43-62.

[25]Simon, M., Hilker, M., 2005. Does rust infection of willow affect feeding and oviposition behavior of willow leaf beetles? J. Insect Behav., 18(1):115-129.

[26]Stout, M.J., Fidantsef, A.L., Duffey, S.S., et al., 1999. Signal interactions in pathogen and insect attack: systemic plant-mediated interactions between pathogens and herbivores of the tomato, Lycopersicon esculentum. Physiol. Mol. Plant Pathol., 54(3-4):115-130.

[27]Strange, R.N., 2003. Introduction to Plant Pathology. John Wiley & Sons, Chichester, UK, p.8-37.

[28]Stribley, M.F., Moores, G.D., Devonshire, A.L., et al., 1983. Application of the FAO-recommended method for detecting insecticide resistance in Aphis jabae Scopoli, Sitobion avenae (F.), Metopolophium dirhodum (Walker) and Rhopalosiphum padi (L.) (Hemiptera: Aphididae). Bull. Entomol. Res., 73(1):107-115.

[29]Strobel, G., Kenfield, D., Bunkers, G., et al., 1991. Phytotoxins as potential herbicides. Experientia, 47(8):819-826.

[30]Sugimoto, N., Osakabe, M., 2014. Cross-resistance between cyenopyrafen and pyridaben in the twospotted spider mite Tetranychus urticae (Acari: Tetranychidae). Pest Manag. Sci., 70(7):1090-1096.

[31]Thomma, B.P.H.J., 2003. Alternaria spp.: from general saprophyte to specific parasite. Mol. Plant Pathol., 4(4):225-236.

[32]Wagner, S., Boyle, C., 1995. Changes in carbohydrate, protein and chlorophyll content, and enzyme activity during the switch from uredinio-to teliospore sporulation in the bean-rust fungus Uromyces appendiculatus (Pers.) Link. J. Phytopathol., 143(11-12):633-638.

[33]Xiao, C., Gregg, P.C., Hu, W., et al., 2002. Attraction of the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), to volatiles from wilted leaves of a non-host plant, Pterocarya stenoptera. Appl. Entomol. Zool., 37(1):1-6.

[34]Yang, F.Z., Li, Y., Yang, B., 2013. The inhibitory effects of rose powdery mildew infection on the oviposition behaviour and performance of beet armyworms. Entomol. Exp. Appl., 148(1):39-47.

[35]Yekeler, H., Bitmis, K., Özcelik, N., et al., 2001. Analysis of toxic effects of Alternaria toxins on esophagus of mice by light and electron microscopy. Toxicol. Pathol., 29(4):492-497.

[36]Zhang, L.H., 2003. Quorum quenching and proactive host defense. Trends Plant Sci., 8(5):238-244.

[37]Zhao, J., Sakai, K., 2003. Multiple signalling pathways mediate fungal elicitor-induced β-thujaplicin biosynthesis in Cupressus lusitanica cell cultures. J. Exp. Bot., 54(383):647-656.

[38]Zhou, B., Qiang, S., 2007. Degradation of tenuazonic acid from Alternaria alternata in soil. J. Agro-Environ. Sci., 26(8):572-576 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE