CLC number: R394-3
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2015-07-09
Cited: 1
Clicked: 4650
Ning Zhang, Jing Jiang, Yan-li Yang, Zhi-he Wang. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. Journal of Zhejiang University Science B, 2015, 16(10): 845-856.
@article{title="Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit",
author="Ning Zhang, Jing Jiang, Yan-li Yang, Zhi-he Wang",
journal="Journal of Zhejiang University Science B",
volume="16",
number="10",
pages="845-856",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400319"
}
%0 Journal Article
%T Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
%A Ning Zhang
%A Jing Jiang
%A Yan-li Yang
%A Zhi-he Wang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 10
%P 845-856
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400319
TY - JOUR
T1 - Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
A1 - Ning Zhang
A1 - Jing Jiang
A1 - Yan-li Yang
A1 - Zhi-he Wang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 10
SP - 845
EP - 856
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400319
Abstract: In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
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