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CLC number: S541

On-line Access: 2015-06-08

Received: 2014-11-30

Revision Accepted: 2015-05-04

Crosschecked: 2015-05-20

Cited: 2

Clicked: 3350

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xin-ping Liang

http://orcid.org/0000-0002-8886-8992

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.479-486

http://doi.org/10.1631/jzus.B1400343


Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells


Author(s):  Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi

Affiliation(s):  College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Corresponding email(s):   annysyh@126.com

Key Words:  Alfalfa saponin extract, Hyperlipidemic BRL cells, Cholesterol metabolism, mRNA expression


Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi. Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells[J]. Journal of Zhejiang University Science B, 2015, 16(6): 479-486.

@article{title="Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells",
author="Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi",
journal="Journal of Zhejiang University Science B",
volume="16",
number="6",
pages="479-486",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400343"
}

%0 Journal Article
%T Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells
%A Xin-ping Liang
%A Dong-qiang Zhang
%A Yan-yan Chen
%A Rui Guo
%A Jie Wang
%A Cheng-zhang Wang
%A Ying-hua Shi
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 479-486
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400343

TY - JOUR
T1 - Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells
A1 - Xin-ping Liang
A1 - Dong-qiang Zhang
A1 - Yan-yan Chen
A1 - Rui Guo
A1 - Jie Wang
A1 - Cheng-zhang Wang
A1 - Ying-hua Shi
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 479
EP - 486
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400343


Abstract: 
We studied the effects of alfalfa saponin extract (ASE) on low density lipoprotein receptor (Ldlr), liver X receptor α (LXRα), and farnesoid X receptor (FXR) in normal and hyperlipidemic Buffalo rat liver (BRL) cells. Normal and hyperlipidemic BRL cells were divided into eight groups: normal, or normal cells treated with 50, 100, and 150 mg/L ASE, hyperlipidemic, or hyperlipidemic cells treated with 50, 100, and 150 mg/L ASE. After treatment for 24 h, Ldlr, LXRα, and FXR mRNA expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Data showed that mRNA expression of Ldlr in normal BRL cells was significantly up-regulated by ASE treatment and mRNA expressions of LXRα and FXR were significantly down-regulated both in normal and hyperlipidemic BRL cells after ASE treatment. Thus, ASE might ameliorate hepatic steatosis by regulating genes involved in cholesterol metabolism, including up-regulation of Ldlr as well as down-regulation of LXRα and FXR.

苜蓿皂苷提取物对BRL细胞Ldlr、LXRα和FXRmRNA表达的影响

目的:分析苜蓿皂苷提取物对正常和脂变Buffalo大鼠肝细胞(BRL细胞)中LdlrLXRαFXRmRNA表达的影响,进而初步探讨苜蓿皂苷调节脂质代谢及降低机体胆固醇含量的机制。
创新点:苜蓿皂苷具有调节脂质代谢,降低机体胆固醇含量的作用,但是目前对其机理研究并不多,特别是在细胞水平上。本试验以BRL细胞为对象,研究苜蓿皂苷对胆固醇代谢相关基因表达的影响,从而探究苜蓿皂苷在细胞水平上对胆固醇代谢的调节作用。
方法:通过50%胎牛血清诱导BRL细胞48h建立细胞脂肪变性模型,运用实时荧光定量聚合酶链式反应(qRT-PCR)检测正常和脂变BRL细胞LdlrLXRαFXRmRNA的表达,从而得出各处理组BRL细胞内LdlrLXRαFXRmRNA表达的变化。
结论:(1)添加苜蓿皂苷后,正常BRL细胞中LdlrmRNA的表达量显著上调,脂变BRL细胞LdlrmRNA的表达有增加的趋势;(2)苜蓿皂苷可以显著下调正常和脂变BRL细胞中LXRαFXRmRNA的表达。因此,在细胞水平上,苜蓿皂苷可能通过促进Ldlr的表达,抑制LXRαFXR的表达,从而调节BRL细胞胆固醇的代谢。

关键词:苜蓿皂苷提取物;脂变BRL细胞;胆固醇代谢;mRNA表达

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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