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CLC number: S541

On-line Access: 2015-06-08

Received: 2014-11-30

Revision Accepted: 2015-05-04

Crosschecked: 2015-05-20

Cited: 2

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Citations:  Bibtex RefMan EndNote GB/T7714


Xin-ping Liang


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.479-486


Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells

Author(s):  Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi

Affiliation(s):  College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Corresponding email(s):   annysyh@126.com

Key Words:  Alfalfa saponin extract, Hyperlipidemic BRL cells, Cholesterol metabolism, mRNA expression

Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi. Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells[J]. Journal of Zhejiang University Science B, 2015, 16(6): 479-486.

@article{title="Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells",
author="Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells
%A Xin-ping Liang
%A Dong-qiang Zhang
%A Yan-yan Chen
%A Rui Guo
%A Jie Wang
%A Cheng-zhang Wang
%A Ying-hua Shi
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 479-486
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400343

T1 - Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells
A1 - Xin-ping Liang
A1 - Dong-qiang Zhang
A1 - Yan-yan Chen
A1 - Rui Guo
A1 - Jie Wang
A1 - Cheng-zhang Wang
A1 - Ying-hua Shi
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 479
EP - 486
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400343

We studied the effects of alfalfa saponin extract (ASE) on low density lipoprotein receptor (Ldlr), liver X receptor α (LXRα), and farnesoid X receptor (FXR) in normal and hyperlipidemic Buffalo rat liver (BRL) cells. Normal and hyperlipidemic BRL cells were divided into eight groups: normal, or normal cells treated with 50, 100, and 150 mg/L ASE, hyperlipidemic, or hyperlipidemic cells treated with 50, 100, and 150 mg/L ASE. After treatment for 24 h, Ldlr, LXRα, and FXR mRNA expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Data showed that mRNA expression of Ldlr in normal BRL cells was significantly up-regulated by ASE treatment and mRNA expressions of LXRα and FXR were significantly down-regulated both in normal and hyperlipidemic BRL cells after ASE treatment. Thus, ASE might ameliorate hepatic steatosis by regulating genes involved in cholesterol metabolism, including up-regulation of Ldlr as well as down-regulation of LXRα and FXR.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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