CLC number: Q819
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2016-03-15
Cited: 1
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Wei Hu, Ji-hong Chen, Shu-yang Wang, Jing Liu, Yuan Song, Qing-feng Wu, Wen-jian Li. Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger[J]. Journal of Zhejiang University Science B, 2016, 17(4): 262-270.
@article{title="Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger",
author="Wei Hu, Ji-hong Chen, Shu-yang Wang, Jing Liu, Yuan Song, Qing-feng Wu, Wen-jian Li",
journal="Journal of Zhejiang University Science B",
volume="17",
number="4",
pages="262-270",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500120"
}
%0 Journal Article
%T Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger
%A Wei Hu
%A Ji-hong Chen
%A Shu-yang Wang
%A Jing Liu
%A Yuan Song
%A Qing-feng Wu
%A Wen-jian Li
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 4
%P 262-270
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500120
TY - JOUR
T1 - Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger
A1 - Wei Hu
A1 - Ji-hong Chen
A1 - Shu-yang Wang
A1 - Jing Liu
A1 - Yuan Song
A1 - Qing-feng Wu
A1 - Wen-jian Li
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 4
SP - 262
EP - 270
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500120
Abstract: The objective of this work was to produce citric acid from corn starch using a newly isolated mutant of Aspergillus , and to analyze the relationship between changes in the physiological properties of A. induced by carbon ion irradiation and citric acid accumulation. Our results showed that the physiological characteristics of conidia in A. niger were closely related to citric acid accumulation and that lower growth rate and viability of conidia may be beneficial to citric acid accumulation. Using corn starch as a raw material, a high-yielding citric acid mutant, named HW2, was obtained. In a 10-L bioreactor, HW2 can accumulate 118.9 g/L citric acid with a residual total sugar concentration of only 14.4 g/L. This represented an 18% increase in citric acid accumulation and a 12.5% decrease in sugar utilization compared with the original strain.
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