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On-line Access: 2021-11-16

Received: 2020-12-17

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 ORCID:

Meilin CUI

https://orcid.org/0000-0003-2800-5770

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.11 P.941-953

http://doi.org/10.1631/jzus.B2000818


Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum


Author(s):  Meilin CUI, Yuchang MA, Youwei YU

Affiliation(s):  College of Food Science, Shanxi Normal University, Taiyuan 030031, China

Corresponding email(s):   cuimeilin1988@163.com

Key Words:  Ganoderma lucidum, Triterpenoid, Heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling, Transcriptome sequencing


Meilin CUI, Yuchang MA, Youwei YU. Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum[J]. Journal of Zhejiang University Science B, 2021, 22(11): 941-953.

@article{title="Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum",
author="Meilin CUI, Yuchang MA, Youwei YU",
journal="Journal of Zhejiang University Science B",
volume="22",
number="11",
pages="941-953",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000818"
}

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%T Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum
%A Meilin CUI
%A Yuchang MA
%A Youwei YU
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000818

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T1 - Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum
A1 - Meilin CUI
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A1 - Youwei YU
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2000818


Abstract: 
Ganoderic triterpenoids (GTs) are the primary bioactive constituents of the Basidiomycotina fungus, Ganoderma lucidum. These compounds exhibit antitumor, anti-hyperlipidemic, and immune-modulatory pharmacological activities. This study focused on GT accumulation in mycelia of G. lucidum mediated by the heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling. Compared with the control, hemin (10 μmol/L) induced an increase of 60.1% in GT content and 57.1% in HO-1 activity. Moreover, carbon monoxide-releasing molecule-2 (CORM-2), CO donor, increased GT content by 56.0% and HO-1 activity by 18.1%. Zn protoporphyrin IX (ZnPPIX), a specific HO-1 inhibitor, significantly reduced GT content by 26.0% and HO-1 activity by 15.8%, while hemin supplementation reversed these effects. transcriptome sequencing showed that HO-1/CO could function directly as a regulator involved in promoting GT accumulation by regulating gene expression in the mevalonate pathway, and modulating the reactive oxygen species (ROS) and Ca2+ pathways. The results of this study may help enhance large-scale GT production and support further exploration of GT metabolic networks and relevant signaling cross-talk.

血红素加氧酶-1/一氧化碳信号参与调控灵芝三萜积累的研究

目的:通过外源性添加血红素加氧酶-1/一氧化碳(HO-1/CO)信号诱导剂及抑制剂,研究了其在灵芝三萜合成过程中的作用,并利用转录组初步探讨作用机制,以期为灵芝三萜代谢网络的深入研究提供参考。
创新点:HO-1/CO信号的研究多见于模式植物,在微生物中较少。本文主要研究了HO-1/CO信号在灵芝菌三萜积累过程中的作用,并初步证实HO-1/CO信号可参与调控三萜合成途径中关键酶基因的表达,同时可影响灵芝菌活性氧(ROS)和钙离子(Ca2+)信号的转导。
方法:本文首先利用HO-1/CO信号诱导剂及抑制剂处理液体发酵的灵芝菌丝,研究了HO-1/CO信号对三萜含量与HO-1酶活及基因表达的影响;其次,研究了HO-1/CO信号对三萜合成途径中关键酶基因(hmgrsqsls)表达的影响;最后,利用转录组测序技术分析HO-1/CO信号介导下灵芝菌的差异表达基因,重点分析了ROS和Ca2+信号中关键基因的变化特征,并进行逆转录聚合酶链反应(RT-PCR)验证。
结论:与未处理相比,血红素(hemin,10μmol/L)与外源性CO释放分子(CORM-2,10μmol/L)处理下三萜含量分别增加60.1%和56.0%;HO-1酶活分别增加57.1%和18.1%;而HO-1特异性抑制剂锌卟啉(ZnPPIX)则显著降低了三萜含量与HO-1酶活。上述各处理对三萜合成途径中关键酶基因(hmgrsqsls)表达均造成不同程度的影响,初步证实HO-1及CO均参与介导三萜合成,且HO-1主要依靠其催化产物CO实现该调控作用。此外,转录组结果显示,HO-1/CO信号在参与介导三萜合成的过程中,还会影响ROS和Ca2+信号的表达。

关键词:灵芝菌;三萜;血红素加氧酶-1/一氧化碳(HO-1/CO)信号;转录组测序

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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