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CLC number: R285.1

On-line Access: 2015-08-04

Received: 2015-05-18

Revision Accepted: 2015-07-22

Crosschecked: 2015-07-24

Cited: 3

Clicked: 2430

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Gui-mei Lin

http://orcid.org/0000-0002-3795-7012

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.8 P.690-695

http://doi.org/10.1631/jzus.B1500121


Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids


Author(s):  Yan-bin Shi, Lian Liu, Wei Shao, Ting Wei, Gui-mei Lin

Affiliation(s):  1School of Mechanical and Automotive Engineering, Qilu University of Technology, Jinan 250353, China; more

Corresponding email(s):   guimeilin@sdu.edu.cn

Key Words:  Aconitum alkaloids, Escherichia coli, Staphylococcus aureus, Microcalorimetry


Yan-bin Shi, Lian Liu, Wei Shao, Ting Wei, Gui-mei Lin. Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids[J]. Journal of Zhejiang University Science B, 2015, 16(8): 690-695.

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author="Yan-bin Shi, Lian Liu, Wei Shao, Ting Wei, Gui-mei Lin",
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doi="10.1631/jzus.B1500121"
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Abstract: 
The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted and applied to Escherichia coli and Staphylococcus aureus. The effect of alkaloids on bacteria growth was studied by microcalorimetry. The power-time curves were plotted with a thermal activity monitor (TAM) air isothermal microcalorimeter and parameters such as growth rate constant (μ), peak-time (Tm), inhibitory ratio (I), and enhancement ratio (E) were calculated. The relationships between the concentration of Aconitum alkaloids and μ of E. coli or S. aureus were discussed. The results showed that Aconitum alkaloids had little effect on E. coli and had a potentially inhibitory effect on the growth of S. aureus.

附子生物碱抗菌活性的微量热研究

目的:研究附子生物碱对大肠杆菌和金黄色葡萄球菌生长代谢作用的影响,并对其抗菌活性进行评价。
创新点:首次应用微量热的方法研究了大肠杆菌和金黄色葡萄球菌在附子生物碱中的生长代谢作用,并从热力学角度对附子生物碱的抗菌活性进行了评价。
方法:微量量热仪绘制了37 °C 时大肠杆菌和金黄色葡萄球菌在不同浓度的附子生物碱作用下的热谱曲线,根据热动力学模型,分别得到了生长速率常数µ、抑制率I、最大产热功率Pm以及最大产热功率所对应的时间Tm等热动力学参数,从热动力学的角度探讨了附子生物碱对大肠杆菌和金黄色葡萄球菌代谢作用的影响。
结论:实验结果显示,附子生物碱对大肠杆菌的生长代谢基本没有影响。但是对于金黄色葡萄球菌,随着附子生物碱浓度的增大,其生长速率常数呈现先升高后降低的趋势,同时达峰时间也被延长,说明附子生物碱对金黄色葡萄球菌存在潜在的生长抑制作用。

关键词:附子生物碱;大肠杆菌;金黄色葡萄球菌;微热量热法

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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