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CLC number: S852.2

On-line Access: 2018-05-05

Received: 2017-01-26

Revision Accepted: 2017-05-09

Crosschecked: 2018-04-19

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Citations:  Bibtex RefMan EndNote GB/T7714


Xing-ting Liu


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.5 P.390-399


Age-related changes of yolk precursor formation in the liver of laying hens

Author(s):  Xing-ting Liu, Xin Lin, Yu-ling Mi, Wei-dong Zeng, Cai-qiao Zhang

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   cqzhang@zju.edu.cn

Key Words:  Lipid metabolism, Yolk precursor formation, 17β, -Estradiol, Antioxidant, Hen

Xing-ting Liu, Xin Lin, Yu-ling Mi, Wei-dong Zeng, Cai-qiao Zhang. Age-related changes of yolk precursor formation in the liver of laying hens[J]. Journal of Zhejiang University Science B, 2018, 19(5): 390-399.

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publisher="Zhejiang University Press & Springer",

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%T Age-related changes of yolk precursor formation in the liver of laying hens
%A Xing-ting Liu
%A Xin Lin
%A Yu-ling Mi
%A Wei-dong Zeng
%A Cai-qiao Zhang
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700054

T1 - Age-related changes of yolk precursor formation in the liver of laying hens
A1 - Xing-ting Liu
A1 - Xin Lin
A1 - Yu-ling Mi
A1 - Wei-dong Zeng
A1 - Cai-qiao Zhang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 5
SP - 390
EP - 399
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700054

A rapid decline in egg production of laying hens begins after 480 d of age. Such a rapid decrease results predominantly from the ovarian aging, accompanied by endocrine changes, decreased yolk synthesis and accumulation, and the reduction in follicles selected into the preovulatory hierarchy. In this study, hens at 90, 150, 280, and 580 d old (D90, D150, D280, and D580, respectively) were compared for yolk precursor formation in the liver to elucidate effects of aging on laying performance. The results showed that liver lipid synthesis increased remarkably in hens from D90 to D150, but decreased sharply at D580 as indicated by the changes in triglyceride (TG) levels. This result was consistent with the age-related changes of the laying performance. The levels of liver antioxidants and total antioxidant capacity decreased significantly in D580 hens and the methane dicarboxylic aldehyde in D580 hens was much higher than that at other stages. The serum 17β;-Estradiol level increased from D90 to D280, but decreased at D580 (P<0.05). The expression of estrogen receptor α and β mRNAs in the liver displayed similar changes to the serum 17β;-Estradiol in D580 hens. Expressions of the genes related to yolk precursor formation and enzymes responsible for fat acid synthesis were all decreased in D580 hens. These results indicated that decreased yolk precursor formation in the liver of the aged hens resulted from concomitant decreases of serum 17β;-Estradiol level, transcription levels of estrogen receptors and critical genes involved in yolk precursor synthesis, and liver antioxidant status.




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[1]Bahr JM, Johnson AL, 1984. Regulation of the follicular hierarchy and ovulation. J Exp Zool, 232(3):495-500.

[2]Bai J, Greene E, Li W, et al., 2015. Branched-chain amino acids modulate the expression of hepatic fatty acid metabolism-related genes in female broiler chickens. Mol Nutr Food Res, 59(6):1171-1181.

[3]Ben-Meir A, Burstein E, Borrego-Alvarez A, et al., 2015. Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging. Aging Cell, 14(5):887-895.

[4]Buyuk E, Nejat E, Neal-Perry G, 2010. Determinants of female reproductive senescence: differential roles for the ovary and the neuroendocrine axis. Semin Reprod Med, 28(5):370-379.

[5]Claire D'Andre H, Paul W, Shen X, et al., 2013. Identification and characterization of genes that control fat deposition in chickens. J Anim Sci Biotechnol, 4(1):43-58.

[6]Cohen A, Smith Y, 2014. Estrogen regulation of microRNAs, target genes, and microRNA expression associated with vitellogenesis in the zebrafish. Zebrafish, 11(5):462-478.

[7]Ge C, Yu M, Zhang C, 2012. G protein-coupled receptor 30 mediates estrogen-induced proliferation of primordial germ cells via EGFR/Akt/β-catenin signaling pathway. Endocrinology, 153(7):3504-3516.

[8]Hall JM, Couse JF, Korach KS, 2001. The multifaceted mechanisms of estradiol and estrogen receptor signaling. J Biol Chem, 276(40):36869-36872.

[9]Hess RA, Fernandes SAF, Gomes GRO, et al., 2011. Estrogen and its receptors in efferent ductules and epididymis. J Androl, 32(6):600-613.

[10]Hoare M, Das T, Alexander G, 2010. Ageing, telomeres, senescence, and liver injury. J Hepatol, 53(5):950-961.

[11]Ishii M, Yamauchi T, Matsumoto K, et al., 2012. Maternal age and reproductive function in female Sprague-Dawley rats. J Toxicol Sci, 37(3):631-638.

[12]Johnson A, 2015. Reproduction in the female. In: Scanes CG (Ed.), Sturkie’s Avian Physiology, 6th Ed. Academic Press, London, p.653-654.

[13]Joyner CJ, Peddie MJ, Taylor TG, 1987. The effect of age on egg production in the domestic hen. Gen Comp Endocrinol, 65(3):331-336.

[14]Khalil HA, Hanafy AM, Saleh SY, et al., 2009. Comparative changes in the serum concentrations of inhibin-B, prolactin, gonadotropins and steroid hormones at different reproductive states in domestic Turkey hens. J Reprod Dev, 55(5):523-528.

[15]Kim CH, Song JH, Lee JC, et al., 2014. Age-related changes in egg quality of Hy-Line brown hens. Int J Poult Sci, 13(9):510-514.

[16]Leaños-Castañeda O, van der Kraak G, 2007. Functional characterization of estrogen receptor subtypes, ERα and ERβ, mediating vitellogenin production in the liver of rainbow trout. Toxicol Appl Pharmacol, 224(2):116-125.

[17]Li H, Wang T, Xu C, et al., 2015. Transcriptome profile of liver at different physiological stages reveals potential mode for lipid metabolism in laying hens. BMC Genomics, 16:763-775.

[18]Li J, Leghari IH, He B, et al., 2014. Estrogen stimulates expression of chicken hepatic vitellogenin II and very low-density apolipoprotein II through ER-α. Theriogenology, 82(3):517-524.

[19]Lillpers K, Wilhelmson M, 1993. Age-dependent changes in oviposition pattern and egg production traits in the domestic hen. Poult Sci, 72(11):2005-2011.

[20]Nagahama Y, 1994. Endocrine regulation of gametogenesis in fish. Int J Dev Biol, 38(2):217-229.

[21]Okudan N, Belviranli M, 2016. Effects of exercise training on hepatic oxidative stress and antioxidant status in aged rats. Arch Physiol Biochem, 122(4):180-185.

[22]Palmer SS, Bahr JM, 1992. Follicle stimulating hormone increases serum oestradiol-17β concentrations, number of growing follicles and yolk deposition in aging hens (Gallus gallus domesticus) with decreased egg production. Br Poult Sci, 33(2):403-414.

[23]Ratna WN, Bhatt VD, Chaudhary K, et al., 2016. Estrogen-responsive genes encoding egg yolk proteins vitellogenin and apolipoprotein II in chicken are differentially regulated by selective estrogen receptor modulators. Theriogenology, 85(3):376-383.

[24]Reddy IJ, David CG, Sarma PV, et al., 2002. The possible role of prolactin in laying performance and steroid hormone secretion in domestic hen (Gallus domesticus). Gen Comp Endocrinol, 127(3):249-255.

[25]Subramanian MV, James TJ, 2010. Age-related protective effect of deprenyl on changes in the levels of diagnostic marker enzymes and antioxidant defense enzymes activities in cerebellar tissue in Wistar rats. Cell Stress Chaperon, 15(5):743-751.

[26]Tong L, Chuang CC, Wu S, et al., 2015. Reactive oxygen species in redox cancer therapy. Cancer Lett, 367(1):18-25.

[27]Verderame M, Limatola E, 2010. Molecular identification of estrogen receptors (ERα and ERβ) and their differential expression during VTG synthesis in the liver of lizard Podarcis sicula. Gen Comp Endocrinol, 168(2):231-238.

[28]Yilmaz O, Prat F, Ibañez AJ, et al., 2015. Estrogen-induced yolk precursors in European sea bass, Dicentrarchus labrax: status and perspectives on multiplicity and functioning of vitellogenins. Gen Comp Endocrinol, 221: 16-22.

[29]Zakaria AH, Miyaki T, Imai K, 1983. The effect of aging on the ovarian follicular growth in laying hens. Poult Sci, 62(4):670-674.

[30]Zhang R, Hu Y, Wang H, et al., 2016. Molecular cloning, characterization, tissue distribution and mRNA expression changes during the hibernation and reproductive periods of estrogen receptor alpha (ESR1) in Chinese alligator, Alligator sinensis. Comp Biochem Physiol B, 200: 28-35.

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