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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Qi-gui Yan

http://orcid.org/0000-0001-5721-2432

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.8 P.610-618

http://doi.org/10.1631/jzus.B1500214


Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China


Author(s):  Cai-fang Wen, Li-xin Xi, Shan Zhao, Zhong-xiang Hao, Lu Luo, Hong Liao, Zhen-rong Chen, Rong She, Guo-quan Han, San-jie Cao, Rui Wu, Qi-gui Yan, Rong Hou

Affiliation(s):  College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; more

Corresponding email(s):   yanqigui@126.com

Key Words:  Chryseobacterium chengduensis, Giant panda, 16S rRNA sequencing, Phylogenetic analysis, Strain 25-1T


Cai-fang Wen, Li-xin Xi, Shan Zhao, Zhong-xiang Hao, Lu Luo, Hong Liao, Zhen-rong Chen, Rong She, Guo-quan Han, San-jie Cao, Rui Wu, Qi-gui Yan, Rong Hou. Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China[J]. Journal of Zhejiang University Science B, 2016, 17(8): 610-618.

@article{title="Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China",
author="Cai-fang Wen, Li-xin Xi, Shan Zhao, Zhong-xiang Hao, Lu Luo, Hong Liao, Zhen-rong Chen, Rong She, Guo-quan Han, San-jie Cao, Rui Wu, Qi-gui Yan, Rong Hou",
journal="Journal of Zhejiang University Science B",
volume="17",
number="8",
pages="610-618",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500214"
}

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%T Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China
%A Cai-fang Wen
%A Li-xin Xi
%A Shan Zhao
%A Zhong-xiang Hao
%A Lu Luo
%A Hong Liao
%A Zhen-rong Chen
%A Rong She
%A Guo-quan Han
%A San-jie Cao
%A Rui Wu
%A Qi-gui Yan
%A Rong Hou
%J Journal of Zhejiang University SCIENCE B
%V 17
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%P 610-618
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500214

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A1 - Cai-fang Wen
A1 - Li-xin Xi
A1 - Shan Zhao
A1 - Zhong-xiang Hao
A1 - Lu Luo
A1 - Hong Liao
A1 - Zhen-rong Chen
A1 - Rong She
A1 - Guo-quan Han
A1 - San-jie Cao
A1 - Rui Wu
A1 - Qi-gui Yan
A1 - Rong Hou
J0 - Journal of Zhejiang University Science B
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SP - 610
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1500214


Abstract: 
A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of giant panda Breeding, China. strain 25-1T grew optimally at pH 7.0–8.0, at 28–30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1T belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81T (96.4% similarity), C. lathyri RBA2-6T (95.8% similarity), and C. zeae JM1085T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA–DNA relatedness between strain 25-1T and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T (CCTCC AB2015133T=DSM 100396T).

分离自成都大熊猫兽舍空气中的一株金黄杆菌属新种的鉴定

目的:鉴定菌株25-1T是否是金黄杆菌属的一个新种。
创新点:首次从空气中分离到金黄杆菌属的新种。
方法:革兰氏染色镜检;磷钨酸染色然后透射电镜观察菌株25-1T形态结构;全自动生理生化鉴定系统(Phoenix™-100)与传统生理生化反应管相结合;H890气象色谱仪进行脂肪酸组分分析;薄板双相层析进行极性脂组分分析;反相高压液相色谱分析法进行呼吸琨组分分析;熔解温度法检测G+C摩尔含量;16S rRNA序列测定及系统发生分析。
结论:根据传统特征分类研究结果(形态特征、培养特性和生理生化特征)、化学分类研究结果(脂肪酸组分、极性脂组分和呼吸琨组分)和遗传特征分类研究结果(G+C摩尔含量、DNA同源性测定和16S rRNA序列测定及系统发生分析)得出菌株25-1T为金黄杆菌属的一个新种。

关键词:成都源金黄杆菌;大熊猫;16S rRNA测序;系统发育树分析;菌株25-1T

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[46]List of electronic supplementary materials

[47]Fig. S1 Transmission electron micrograph of a negatively stained cell of strain 25-1T, showing the absence of flagella

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