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Abstract: A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1^T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of giant panda Breeding, China. strain 25-1^T grew optimally at pH 7.0–8.0, at 28–30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1^T belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81^T (96.4% similarity), C. lathyri RBA2-6^T (95.8% similarity), and C. zeae JM1085^T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C_15:0 (44.0%), iso-C_17:0 3OH (19.8%) and C_16:1 ω7c/_16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA–DNA relatedness between strain 25-1^T and C. lathyri RBA2-6^T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1^T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1^T (CCTCC AB2015133^T=DSM 100396^T).

分离自成都大熊猫兽舍空气中的一株金黄杆菌属新种的鉴定

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[46]List of electronic supplementary materials

[47]Fig. S1 Transmission electron micrograph of a negatively stained cell of strain 25-1^T, showing the absence of flagella