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On-line Access: 2016-12-05

Received: 2016-02-21

Revision Accepted: 2016-05-04

Crosschecked: 2016-11-10

Cited: 1

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Citations:  Bibtex RefMan EndNote GB/T7714


Xian-yong Ma


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.12 P.965-974


Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

Author(s):  Lin Huang, Xian-yong Ma, Zong-yong Jiang, You-jun Hu, Chun-tian Zheng, Xue-fen Yang, Li Wang, Kai-guo Gao

Affiliation(s):  Institute of Animal Science, Guangdong Academy of Agricultural Sciences, the Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Corresponding email(s):   jiangz28@qq.com

Key Words:  Young piglets, Oxidized fish oil, Growth performance, Soybean isoflavone, Antioxidant capacity

Lin Huang, Xian-yong Ma, Zong-yong Jiang, You-jun Hu, Chun-tian Zheng, Xue-fen Yang, Li Wang, Kai-guo Gao. Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil[J]. Journal of Zhejiang University Science B, 2016, 17(12): 965-974.

@article{title="Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil",
author="Lin Huang, Xian-yong Ma, Zong-yong Jiang, You-jun Hu, Chun-tian Zheng, Xue-fen Yang, Li Wang, Kai-guo Gao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil
%A Lin Huang
%A Xian-yong Ma
%A Zong-yong Jiang
%A You-jun Hu
%A Chun-tian Zheng
%A Xue-fen Yang
%A Li Wang
%A Kai-guo Gao
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 12
%P 965-974
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600078

T1 - Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil
A1 - Lin Huang
A1 - Xian-yong Ma
A1 - Zong-yong Jiang
A1 - You-jun Hu
A1 - Chun-tian Zheng
A1 - Xue-fen Yang
A1 - Li Wang
A1 - Kai-guo Gao
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 12
SP - 965
EP - 974
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600078

To investigate the effect of glycitein, a synthetic soybean isoflavone (ISF), on the intestinal antioxidant capacity, morphology, and cytokine content in young piglets fed oxidized fish oil, 72 4-d-old male piglets were assigned to three treatments. The control group was fed a basal diet containing fresh fish oil, and the other two groups received the same diet except for the substitution with the same dosage of oxidized fish oil alone or with ISF (oxidized fish oil plus ISF). After 21 d of feeding, supplementation of oxidized fish oil increased the levels of malondialdehyde (MDA), oxidized glutathione (GSSG), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), nuclear factor κ B (NF-κB), inducible nitric oxide synthase (iNOS), NO, and Caspase-3 in jejunal mucosa, and decreased the villous height in duodenum and the levels of secretory immunoglobulin A (sIgA) and IL-4 in the jejunal mucosa compared with supplementation with fresh oil. The addition of oxidized fish oil plus ISF partially alleviated this negative effect. The addition of oxidized fish oil plus ISF increased the villous height and levels of sIgA and IL-4 in jejunal mucosa, but decreased the levels of IL-1β and IL-2 in jejunal mucosa (P<0.05) compared with oxidized fish oil. Collectively, these results show that dietary supplementation of ISF could partly alleviate the negative effect of oxidized fish oil by improving the intestinal morphology as well as the antioxidant capacity and immune function in young piglets.


方法:将72头平均体重约1.8 kg的4日龄杜×(长×大)三元杂公猪随机分为3组,每组重复6次,每次重复包括4头猪:对照组(饲喂50 g/kg新鲜鱼油),处理组1(添加50 g/kg氧化鱼油)和处理组2(添加50 g/kg氧化鱼油+20 mg/kg大豆异黄酮);基础日粮根据NRC(2012)《猪的营养需要量》配制,每3小时饲喂一次,记录每天采食量;实验期21天结束后,称重,计算平均日采食量、平均日增重及料重比。用苏木精-伊红染色法观察小肠形态结构,并计算绒毛高度和隐窝深度,应用南京建成试剂盒测定肠道中抗氧化酶的活性,应用酶联免疫吸附测定(ELISA)试剂盒测定细胞炎症相关因子和细胞凋亡相关因子的表达。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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