CLC number: R587.1
On-line Access: 2017-03-08
Received: 2016-05-31
Revision Accepted: 2016-08-26
Crosschecked: 2017-02-01
Cited: 0
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Xiao-fen He, Jun-jun Wei, Sheng-yun Shou, Jian-qiao Fang, Yong-liang Jiang. Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion[J]. Journal of Zhejiang University Science B, 2017, 18(3): 239-248.
@article{title="Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion",
author="Xiao-fen He, Jun-jun Wei, Sheng-yun Shou, Jian-qiao Fang, Yong-liang Jiang",
journal="Journal of Zhejiang University Science B",
volume="18",
number="3",
pages="239-248",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600247"
}
%0 Journal Article
%T Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion
%A Xiao-fen He
%A Jun-jun Wei
%A Sheng-yun Shou
%A Jian-qiao Fang
%A Yong-liang Jiang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 3
%P 239-248
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600247
TY - JOUR
T1 - Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion
A1 - Xiao-fen He
A1 - Jun-jun Wei
A1 - Sheng-yun Shou
A1 - Jian-qiao Fang
A1 - Yong-liang Jiang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 3
SP - 239
EP - 248
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600247
Abstract: Objective: To investigate the analgesic effects of electroacupuncture (EA) at 2 and 100 Hz on type 2 diabetic neuropathic pain (DNP) and on the expressions of the p2X3 receptor and calcitonin gene-related peptide (CGRP) in the dorsal root ganglion (DRG). Methods: Rat type 2 DNP was induced by a high calorie and high sugar diet fed for 7 weeks, plus a single intraperitoneal injection of streptozotocin (STZ) after 5 weeks. EA at 2 and 100 Hz was carried out once every day after 7 weeks for 7 consecutive days. Body weight, serum fasting insulin (FINS), fasting blood glucose (FBG), insulin sensitivity index (ISI), and paw withdrawal latency (PWL) were measured. The expressions of L4–L6 DRG p2X3 receptors and CGRP were assessed by immunofluorescence. Results: Type 2 DNP was successfully induced as shown by the increased body weight, FINS, and FBG, as well as the reduced ISI and PWL. Expressions of p2X3 receptors and CGRP in L4–L6 DRGs increased. EA at both 2 and 100 Hz relieved type 2 DNP, but the analgesic effect of EA was stronger at 2 Hz. p2X3 receptor expression decreased in L4–L6 DRGs following EA at 2 Hz and in L5 and L6 DRGs following EA at 100 Hz. EA at both 2 and 100 Hz down-regulated CGRP overexpression in L4–L6 DRGs. Conclusions: These findings indicate that EA at 2 Hz is a good option for the management of type 2 DNP. The EA effect may be related to its down-regulation of the overexpressions of the DRG p2X3 receptors and CGRP in this condition.
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