CLC number: R781.6+4
On-line Access: 2020-03-02
Received: 2019-07-29
Revision Accepted: 2019-10-25
Crosschecked: 2020-02-04
Cited: 0
Clicked: 3433
Cheng-ming Ni, He-ping Sun, Xiang Xu, Bing-yu Ling, Hui Jin, Yu-qiu Zhang, Zhi-qi Zhao, Hong Cao, Lan Xu. Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice[J]. Journal of Zhejiang University Science B, 2020, 21(2): 155-165.
@article{title="Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice",
author="Cheng-ming Ni, He-ping Sun, Xiang Xu, Bing-yu Ling, Hui Jin, Yu-qiu Zhang, Zhi-qi Zhao, Hong Cao, Lan Xu",
journal="Journal of Zhejiang University Science B",
volume="21",
number="2",
pages="155-165",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900456"
}
%0 Journal Article
%T Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice
%A Cheng-ming Ni
%A He-ping Sun
%A Xiang Xu
%A Bing-yu Ling
%A Hui Jin
%A Yu-qiu Zhang
%A Zhi-qi Zhao
%A Hong Cao
%A Lan Xu
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 2
%P 155-165
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900456
TY - JOUR
T1 - Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice
A1 - Cheng-ming Ni
A1 - He-ping Sun
A1 - Xiang Xu
A1 - Bing-yu Ling
A1 - Hui Jin
A1 - Yu-qiu Zhang
A1 - Zhi-qi Zhao
A1 - Hong Cao
A1 - Lan Xu
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 2
SP - 155
EP - 165
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900456
Abstract: Painful diabetic neuropathy (PDN) is a diabetes mellitus complication. Unfortunately, the mechanisms underlying PDN are still poorly understood. Adenosine triphosphate (ATP)-gated p2X7 receptor (P2X7R) plays a pivotal role in non-diabetic neuropathic pain, but little is known about its effects on streptozotocin (STZ)-induced peripheral neuropathy. Here, we explored whether spinal cord P2X7R was correlated with the generation of mechanical allodynia (MA) in STZ-induced type 1 diabetic neuropathy in mice. MA was assessed by measuring paw withdrawal thresholds and western blotting. Immunohistochemistry was applied to analyze the protein expression levels and localization of P2X7R. STZ-induced mice expressed increased P2X7R in the dorsal horn of the lumbar spinal cord during MA. Mice injected intrathecally with a selective antagonist of P2X7R and P2X7R knockout (KO) mice both presented attenuated progression of MA. Double-immunofluorescent labeling demonstrated that P2X7R-positive cells were mostly co-expressed with Iba1 (a microglia marker). Our results suggest that P2X7R plays an important role in the development of MA and could be used as a cellular target for treating PDN.
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