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On-line Access: 2017-06-05

Received: 2016-06-27

Revision Accepted: 2016-10-05

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Xue-fen Yang


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.492-500


CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs

Author(s):  Yue-qin Qiu, Xue-fen Yang, Xian-yong Ma, Yun-xia Xiong, Zhi-mei Tian, Qiu-li Fan, Li Wang, Zong-yong Jiang

Affiliation(s):  Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Corresponding email(s):   yangxuefen@gdaas.cn

Key Words:  Cell death-inducing DNA fragmentation factor α, -like effector (CIDE), Adipose tissue, Liver, Skeletal muscle, Fat deposition, Lantang pig, DLY pig

Yue-qin Qiu, Xue-fen Yang, Xian-yong Ma, Yun-xia Xiong, Zhi-mei Tian, Qiu-li Fan, Li Wang, Zong-yong Jiang. CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs[J]. Journal of Zhejiang University Science B, 2017, 18(6): 492-500.

@article{title="CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs",
author="Yue-qin Qiu, Xue-fen Yang, Xian-yong Ma, Yun-xia Xiong, Zhi-mei Tian, Qiu-li Fan, Li Wang, Zong-yong Jiang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs
%A Yue-qin Qiu
%A Xue-fen Yang
%A Xian-yong Ma
%A Yun-xia Xiong
%A Zhi-mei Tian
%A Qiu-li Fan
%A Li Wang
%A Zong-yong Jiang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 6
%P 492-500
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600294

T1 - CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs
A1 - Yue-qin Qiu
A1 - Xue-fen Yang
A1 - Xian-yong Ma
A1 - Yun-xia Xiong
A1 - Zhi-mei Tian
A1 - Qiu-li Fan
A1 - Li Wang
A1 - Zong-yong Jiang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 6
SP - 492
EP - 500
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600294

The expression of the cell death-inducing DNA fragmentation factor α;-like effector (CIDE) family including Cidea, Cideb, and Cidec was significantly increased in mouse and human models of obesity. However, there was less information on these genes’ expression in pigs. Here, we hypothesized that different fat accumulation between lean (Duroc×Landrace×Yorkshire gilts, DLY) and obese (Lantang) pigs was attributed to porcine CIDE-modulating lipid metabolism. Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue, and at a relatively high level in skeletal muscle, whereas Cideb was mainly expressed in the liver in both breeds of pig. lantang pigs had higher white adipose and skeletal muscle Cidea and Cidec mRNA abundance, and hepatic and muscle Cideb mRNA than DLY pigs. Lipid metabolism-related genes including sterol regulatory element binding protein 1c (SREBP-1c), hepatocyte nuclear factor-4α (HNF-4α), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), fatty acid synthase (FASN), diacylglycerol O-acyltransferase 1 (DGAT1), and DGAT2 showed a higher expression level in adipose tissue from obese pigs than in that from lean pigs. lantang pigs exhibited higher mRNA abundance for liver SREBP-1c, HNF-4α, and PGC-1α, and higher skeletal muscle SREBP-1c, HNF-4α, PGC-1α, and DGAT2 expression, as compared with DLY pigs. However, the perlipin2 mRNA levels in adipose tissues, liver, and skeletal muscle were significantly lower in obese pigs than in their lean counterparts. Furthermore, plasma non-esterified fatty acid (NEFA), glucose, and triacylglycerol (TAG) levels were greater in obese pigs than in lean pigs. Finally, data from correlation analysis further found that CIDE mRNA expression was positively correlated with back fat thickness (BFT), abdominal fat mass (AFM), and the levels of NEFA, TAG, and glucose in the two breeds. Collectively, these data revealed that the porcine CIDEs possibly modulated lipid metabolism and contributed to the development of fat deposition and obesity in lantang pigs.


方法:采用荧光定量聚合酶链式反应(qPCR)检测肥胖型蓝塘猪和瘦肉型杜长大猪的脂肪、肝脏和骨骼肌中CIDE家族基因、SREBP-1cPGC-1αHNF-4αFASNDGAT1DGAT2perlipin 2等基因表达水平。采用血浆生化指标仪试剂盒检测两个品种猪血浆中甘油三酯、葡萄糖、游离脂肪酸及胆固醇的含量。
结论:肥胖型蓝塘猪脂肪和背最长肌组织中的CideaCidec,及肝脏中Cidec的基因表达量明显高于瘦肉型杜长大猪。在脂肪组织中,脂质代谢相关的基因(包括SREBP-1cPGC-1αHNF-4αFASNDGAT1DGAT2基因)表达量都是蓝塘猪高于杜长大猪。蓝塘猪肝脏中的SREBP-1cHNF-4αPGC-1α基因表达水平显著高于杜长大猪。蓝塘猪背最长肌组织的SREBP-1cHNF-4αPGC-1αDGAT2基因表达量高于杜长大猪。然而,蓝塘猪的脂肪、肝脏及背最长肌三种组织中的perlipin 2的表达量显著低于杜长大猪。此外,蓝塘猪血浆中的甘油三酯、葡萄糖及游离脂肪酸浓度明显高于杜长大猪。通过相关性分析,我们发现肥胖型和瘦肉型猪不同组织中的CIDE家族基因表达水平与背部脂肪厚度、腹部脂肪重量、血浆中的甘油三酯、葡萄糖及游离脂肪酸浓度有明显的正向相关性。综上所述,CIDE家族基因可以调节脂质代谢,并促进脂肪沉积及导致肥胖。


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