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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.11 P.877-890

http://doi.org/10.1631/jzus.B1800530


Effects of dietary glycerol monolaurate on productive performance, egg quality, serum biochemical indices, and intestinal morphology of laying hens


Author(s):  Min-Jie Zhao, Hai-Ying Cai, Meng-Yun Liu, Ling-Li Deng, Yang Li, Hui Zhang, Feng-Qin Feng

Affiliation(s):  College of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   fengfq@zju.edu.cn

Key Words:  Glycerol monolaurate (GML), Laying hen, Productive performance, Egg quality, Lipid metabolism


Min-Jie Zhao, Hai-Ying Cai, Meng-Yun Liu, Ling-Li Deng, Yang Li, Hui Zhang, Feng-Qin Feng. Effects of dietary glycerol monolaurate on productive performance, egg quality, serum biochemical indices, and intestinal morphology of laying hens[J]. Journal of Zhejiang University Science B, 2019, 20(11): 877-890.

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author="Min-Jie Zhao, Hai-Ying Cai, Meng-Yun Liu, Ling-Li Deng, Yang Li, Hui Zhang, Feng-Qin Feng",
journal="Journal of Zhejiang University Science B",
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number="11",
pages="877-890",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800530"
}

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%T Effects of dietary glycerol monolaurate on productive performance, egg quality, serum biochemical indices, and intestinal morphology of laying hens
%A Min-Jie Zhao
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%A Ling-Li Deng
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%A Hui Zhang
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A1 - Ling-Li Deng
A1 - Yang Li
A1 - Hui Zhang
A1 - Feng-Qin Feng
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DOI - 10.1631/jzus.B1800530


Abstract: 
This article has been retracted. Please see https://doi.org/10.1631/jzus.B18r0530 glycerol monolaurate (GML) has been widely used as an effective antibacterial emulsifier in the food industry. A total of 360 44-week-old Hy-Line brown laying hens were randomly distributed into four groups each with six replicates of 15 birds, and fed with corn-soybean-meal-based diets supplemented with 0, 0.15, 0.30, and 0.45 g/kg GML, respectively. Our results showed that 0.15, 0.30, and 0.45 g/kg GML treatments significantly decreased feed conversion ratios (FCRs) by 2.65%, 7.08%, and 3.54%, respectively, and significantly increased the laying rates and average egg weights. For egg quality, GML drastically increased albumen height and Haugh units, and enhanced yolk color. Notably, GML increased the concentrations of polyunsaturated and monounsaturated fatty acids and reduced the concentration of total saturated fatty acids in the yolk. The albumen composition was also significantly modified, with an increase of 1.02% in total protein content, and increased contents of His (4.55%) and Glu (2.02%) under the 0.30 g/kg GML treatment. Additionally, GML treatments had positive effects on the lipid metabolism of laying hens, including lowering the serum triglyceride and total cholesterol levels and reducing fat deposition in abdominal adipose tissue. Intestinal morphology was also improved by GML treatment, with increased villus length and villus height to crypt depth ratio. Our data demonstrated that GML supplementation of laying hens could have beneficial effects on both their productivity and physiological properties, which indicates the potential application of GML as a functional feed additive and gives us a new insight into this traditional food additive.

饲粮中添加月桂酸单甘油酯对蛋鸡产蛋性能、蛋品质、血清生化指标以及小肠形态的影响

目的:基于传统食品添加剂月桂酸单甘油酯(GML)的抗菌、抗病毒和免疫调节特性,研究其对蛋鸡产蛋性能、蛋品质和生理健康的影响.
创新点:将传统食品添加剂GML应用于蛋鸡养殖行业,探究其作为饲粮添加剂改善动物营养健康状况的潜能.
方法:将360只海兰褐蛋鸡随机分为4组,每组6个重复,每个重复15只蛋鸡,分别饲喂以玉米-豆粕为主的日粮,并在日粮中分别添加0、0.15、0.30和0.45 g/kg GML.饲喂8周后,测定蛋鸡的产蛋性能、蛋品质、蛋清以及蛋黄相关营养成分组成、血清生理生化以及激素相关指标,并对空肠和附睾脂肪切片进行形态学观察,系统研究GML对蛋鸡的影响.
结论:0.15、0.30和0.45 g/kg GML处理能显著降低饲料转化率,并显著提高产蛋率和平均蛋重.在鸡蛋品质方面,GML显著提高了蛋白的高度和哈夫单位,并增强了蛋黄的颜色.此外,GML还增加了蛋黄中多不饱和脂肪酸和单不饱和脂肪酸的浓度,降低了蛋黄中总饱和脂肪酸的浓度.蛋白组成在GML作用下也有显著改变,其中0.30 g/kg GML显著增加了总蛋白含量以及组氨酸和谷氨酸的含量.GML对蛋鸡脂代谢也表现除了积极作用,能显著降低血清甘油三酯和总胆固醇水平,减少腹部脂肪组织的沉积.在改善肠道形态方面,GML增加了绒毛长度以及绒毛高度与隐窝深度的比值.总体来说,GML对蛋鸡的生产性能和生理特性均有显著的改善作用,表明GML有作为功能性饲料添加剂的潜能,拓宽了传统食品添加剂GML的应用领域.

关键词:月桂酸单甘油酯(GML);蛋鸡;产蛋性能;蛋品质;脂代谢

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Akter Y, Greenhalgh S, Islam MR, et al., 2018. Hens ranked as highly feed efficient have an improved albumen quality profile and increased polyunsaturated fatty acids in the yolk. J Anim Sci, 96(8):3482-3490.

[2]Basmacioglu H, Cabuk M, Unal K, et al., 2003. Effects of dietary fish oil and flax seed on cholesterol and fatty acid composition of egg yolk and blood parameters of laying hens. S Afr J Anim Sci, 33(4):266-273.

[3]Batovska DI, Todorova IT, Tsvetkova IV, et al., 2009. Antibacterial study of the medium chain fatty acids and their 1-monoglycerides: individual effects and synergistic relationships. Pol J Microbiol, 58(1):43-47.

[4]Bonilla CEV, Rosa AP, Londero A, et al., 2017. Effect of broiler breeders fed with corn or sorghum diet and canthaxanthin supplementation on production and reproductive performance. Poult Sci, 96(6):1725-1734.

[5]Chassaing B, Koren O, Goodrich JK, et al., 2015. Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome. Nature, 519(7541):92-96.

[6]Chiang SH, Huang KH, Lee HF, 1990. Effects of medium chain triglyceride on energy metabolism, growth and body fat in broilers. J Chin Soc Anim Sci, 19(1-2):11-19 (in Chinese).

[7]Coballase-Urrutia E, Pedraza-Chaverri J, Cárdenas-Rodríguez N, et al., 2011. Hepatoprotective effect of acetonic and methanolic extracts of Heterotheca inuloides against CCL4-induced toxicity in rats. Exp Toxicol Pathol, 63(4): 363-370.

[8]Dierick NA, Decuypere JA, Degeyter I, 2003. The combined use of whole Cuphea seeds containing medium chain fatty acids and an exogenous lipase in piglet nutrition. Arch Anim Nutr, 57(1):49-63.

[9]Famurewa AC, Aja PM, Maduagwuna EK, et al., 2017. Antioxidant and anti-inflammatory effects of virgin coconut oil supplementation abrogate acute chemotherapy oxidative nephrotoxicity induced by anticancer drug methotrexate in rats. Biomed Pharmacother, 96:905-911.

[10]Furuse M, Mabayo RT, Kita K, et al., 1992. Effect of dietary medium chain triglyceride on protein and energy utilisation in growing chicks. Br Poult Sci, 33(1):49-57.

[11]Geng SS, Zhu WW, Xie CF, et al., 2016. Medium-chain triglyceride ameliorates insulin resistance and inflammation in high fat diet-induced obese mice. Eur J Nutr, 55(3): 931-940.

[12]Hou SM, He HB, Zhang W, et al., 2009. Determination of soil amino acids by high performance liquid chromatography-electro spray ionization-mass spectrometry derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Talanta, 80(2):440-447.

[13]Isaacs CE, Litov RE, Thormar H, 1995. Antimicrobial activity of lipids added to human milk, infant formula, and bovine milk. J Nutr Biochem, 6(7):362-366.

[14]Ishibashi T, Yonemochi C, 2003. Amino acid nutrition in egg production industry. Anim Sci J, 74(6):457-469.

[15]Jin TZ, Gurtler JB, Li SQ, 2013. Development of antimicrobial coatings for improving the microbiological safety and quality of shell eggs. J Food Prot, 76(5):779-785.

[16]Kim J, Choi YH, 2014. Differential abundance of egg white proteins in laying hens treated with corticosterone. J Agric Food Chem, 62(51):12346-12359.

[17]Lee SI, Kim HS, Kim I, 2015. Microencapsulated organic acid blend with MCFAs can be used as an alternative to antibiotics for laying hens. Turk J Vet Anim Sci, 39(5):520-527.

[18]Meirhaeghe HV, Rogge T, Lopez A, et al., 2015. Efficacy of butyric acid glycerides and glycerol monolaurate to combat bacterial enteritis problems in broilers. Actes des 11èmes Journées de la Recherche Avicole et Palmipèdes à Foie Gras, Tours, France, p.712-716 (in French).

[19]Milinsk MC, Murakami AE, Gomes STM, et al., 2003. Fatty acid profile of egg yolk lipids from hens fed diets rich in n-3 fatty acids. Food Chem, 83(2):287-292.

[20]Mirbod M, Mahdavi AH, Samie AH, et al., 2017. Effects of Curcuma longa rhizome powder on egg quality, performance and some physiological indices of laying hens fed different levels of metabolizable energy. J Sci Food Agric, 97(4):1286-1294.

[21]Mumme K, Stonehouse W, 2015. Effects of medium-chain triglycerides on weight loss and body composition: a meta-analysis of randomized controlled trials. J Acad Nutr Diet, 115(2):249-263.

[22]Nam KC, Du M, Jo C, et al., 2001. Cholesterol oxidation products in irradiated raw meat with different packaging and storage time. Meat Sci, 58(4):431-435.

[23]Odle J, 1997. New insights into the utilization of medium-chain triglycerides by the neonate: observations from a piglet model. J Nutr, 127(6):1061-1067.

[24]Pesti GM, 1995. Nutrient requirements of poultry. Anim Feed Sci Technol, 56(1):177-178.

[25]Sakanaka S, Kitahata K, Mitsuya T, et al., 2000. Protein quality determination of delipidated egg-yolk. J Food Compos Anal, 13(5):773-781.

[26]Schlievert PM, Peterson ML, 2012. Glycerol monolaurate antibacterial activity in broth and biofilm cultures. PLoS ONE, 7(7):e40350.

[27]Schlievert PM, Deringer JR, Kim MH, et al., 1992. Effect of glycerol monolaurate on bacterial growth and toxin production. Antimicrob Agents Chemother, 36(3):626-631.

[28]Shokrollahi B, Yavari Z, Kordestani AH, 2014. Effects of dietary medium-chain fatty acids on performance, carcass characteristics, and some serum parameters of broiler chickens. Br Poult Sci, 55(5):662-667.

[29]Skřivan M, Dlouhá G, Englmaierová M, et al., 2010. Effects of different levels of dietary supplemental caprylic acid and vitamin E on performance, breast muscle vitamin E and A, and oxidative stability in broilers. Czech J Anim Sci, 55(4): 167-173.

[30]https://doi.org/10.17221/221/2009-CJAS

[31]Sun H, Lee EJ, Samaraweera H, et al., 2013. Effects of increasing concentrations of corn distillers dried grains with solubles on chemical composition and nutrient content of egg. Poult Sci, 92(1):233-242.

[32]Thacker PA, 2013. Alternatives to antibiotics as growth promoters for use in swine production: a review. J Anim Sci Biotechnol, 4(1):35.

[33]Thomsen KV, 1966. The influence of coconut fat and soybean oil meals on the fatty acid composition of hens’ eggs. Acta Agric Scand, 16(3-4):194-198.

[34]Thomsen KV, 1967. Fatty acid composition of egg lipids derived from hens receiving diets of soybean oil and coconut fat conjunctively. Acta Agric Scand, 17(1):53-57.

[35]Thormar H, Isaacs CE, Brown HR, et al., 1987. Inactivation of enveloped viruses and killing of cells by fatty acids and monoglycerides. Antimicrob Agents Chemother, 31(1): 27-31.

[36]van der Aar PJ, Molist F, van der Klis JD, 2017. The central role of intestinal health on the effect of feed additives on feed intake in swine and poultry. Anim Feed Sci Technol, 233:64-75.

[37]van der Hoeven-Hangoor E, van der Vossen JMBM, Schuren FHJ, et al., 2013. Ileal microbiota composition of broilers fed various commercial diet compositions. Poult Sci, 92(10):2713-2723.

[38]Wang JH, Wang XX, Li JT, et al., 2015. Effects of dietary coconut oil as a medium-chain fatty acid source on performance, carcass composition and serum lipids in male broilers. Asian-Australas J Anim Sci, 28(2):223-230.

[39]Wang JP, Kim IH, 2011. Effect of caprylic acid and Yucca schidigera extract on production performance, egg quality, blood characteristics, and excreta microflora in laying hens. Br Poult Sci, 52(6):711-717.

[40]Wieland M, Weber BK, Hafner-Marx A, et al., 2015. A controlled trial on the effect of feeding dietary chestnut extract and glycerol monolaurate on liver function in newborn calves. J Anim Physiol Anim Nutr, 99(1):190-200.

[41]Witcher KJ, Novick RP, Schlievert PM, 1996. Modulation of immune cell proliferation by glycerol monolaurate. Clin Diagn Lab Immunol, 3(1):10-13.

[42]Yuniwarti EYW, Asmara W, Artama WT, et al., 2012. Virgin coconut oil increases the productivity of broiler chicken post avian influenza vaccination. Anim Prod, 14(3):192-198.

[43]Zeitz JO, Fennhoff J, Kluge H, et al., 2015. Effects of dietary fats rich in lauric and myristic acid on performance, intestinal morphology, gut microbes, and meat quality in broilers. Poult Sci, 94(10):2404-2413.

[44]Zentek J, Buchheit-Renko S, Ferrara F, et al., 2011. Nutritional and physiological role of medium-chain triglycerides and medium-chain fatty acids in piglets. Anim Health Res Rev, 12(1):83-93.

[45]Zentek J, Buchheit-Renko S, Männer K, et al., 2012. Intestinal concentrations of free and encapsulated dietary medium-chain fatty acids and effects on gastric microbial ecology and bacterial metabolic products in the digestive tract of piglets. Arch Anim Nutr, 66(1):14-26.

[46]Zhang M, Zou XT, Li H, et al., 2012. Effect of dietary γ-aminobutyric acid on laying performance, egg quality, immune activity and endocrine hormone in heat-stressed roman hens. Anim Sci J, 83(2):141-147.

[47]Zhang XS, Zhang Y, Liu YH, et al., 2016. Medium-chain triglycerides promote macrophage reverse cholesterol transport and improve atherosclerosis in ApoE-deficient mice fed a high-fat diet. Nutr Res, 36(9):964-973.

[48]Zhou SM, Wang YQ, Jacoby JJ, et al., 2017. Effects of medium- and long-chain triacylglycerols on lipid metabolism and gut microbiota composition in C57bL/6J mice. J Agric Food Chem, 65(31):6599-6607.

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