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On-line Access: 2018-06-04

Received: 2017-05-03

Revision Accepted: 2017-08-09

Crosschecked: 2018-05-14

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Hui Li


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.6 P.445-457


Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content

Author(s):  Hui Li, Mei-hua Sun, Ming-fang Qi, Jiao Xing, Tao Xu, Han-ting Liu, Tian-lai Li

Affiliation(s):  Key Laboratory of Protected Horticulture of Liaoning Province / Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region / Horticulture Department, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding email(s):   2671329915@qq.com, tianlaili@126.com

Key Words:  SlYABBY2b, Gibberellin, Gibberellin-deficient mutants, Ovary locule number, Transgenic plant, Gene expression, Tomato

Hui Li, Mei-hua Sun, Ming-fang Qi, Jiao Xing, Tao Xu, Han-ting Liu, Tian-lai Li. Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content[J]. Journal of Zhejiang University Science B, 2018, 19(6): 445-457.

@article{title="Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content",
author="Hui Li, Mei-hua Sun, Ming-fang Qi, Jiao Xing, Tao Xu, Han-ting Liu, Tian-lai Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content
%A Hui Li
%A Mei-hua Sun
%A Ming-fang Qi
%A Jiao Xing
%A Tao Xu
%A Han-ting Liu
%A Tian-lai Li
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 6
%P 445-457
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700238

T1 - Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content
A1 - Hui Li
A1 - Mei-hua Sun
A1 - Ming-fang Qi
A1 - Jiao Xing
A1 - Tao Xu
A1 - Han-ting Liu
A1 - Tian-lai Li
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 6
SP - 445
EP - 457
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700238

tomato is an ideal model species for fleshy fruit development research. SlYABBY2b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between SlYABBY2b and endogenous gibberellin is poorly understood. In this study, SlYABBY2b-overexpressing and RNA interference (RNAi) transgenic tomato plants were used to elucidate the mechanism by which SlYABBY2b regulates the ovary locule number and endogenous gibberellin content in tomato. SlYABBY2b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the SlYABBY2b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of SlYABBY2b gene was remarkably higher than that of the wild type in the apical shoots of gibberellin-deficient mutants. This showed that the gibberellins can inhibit the expression of SlYABBY2b gene negative regulation. Further study revealed that SlYABBY2b suppressed the expression of SlGA20ox1 and SlGA3ox2, but increased that of SlGA2ox1 and SlGA2ox5 in the apical shoots of SlYABBY2b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the SlYABBY2b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule.

SlYABBY2b基因对番茄果实心室数和内源赤霉 素含量的影响



Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[50]Table S1 Gibberellin mutants and wild types

[51]Table S2 RT-PCR primers used to amplify gene-specific regions

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