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CLC number: Q319

On-line Access: 2018-08-02

Received: 2017-08-19

Revision Accepted: 2017-10-22

Crosschecked: 2018-07-10

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Citations:  Bibtex RefMan EndNote GB/T7714


Qing-Yao Shu


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.620-629


High-resolution melting-based TILLING of γ ray-induced mutations in rice

Author(s):  Shan Li, Song-Mei Liu, Hao-Wei Fu, Jian-Zhong Huang, Qing-Yao Shu

Affiliation(s):  National Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   qyshu@zju.edu.cn

Key Words:  Mutation screening, High-resolution melting (HRM) analysis, Targeting Induced Local Lesions IN Genomes (TILLING), Mutant, Indel, γ, ray, Rice

Shan Li, Song-Mei Liu, Hao-Wei Fu, Jian-Zhong Huang, Qing-Yao Shu. High-resolution melting-based TILLING of γ ray-induced mutations in rice[J]. Journal of Zhejiang University Science B, 2018, 19(8): 620-629.

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author="Shan Li, Song-Mei Liu, Hao-Wei Fu, Jian-Zhong Huang, Qing-Yao Shu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T High-resolution melting-based TILLING of γ ray-induced mutations in rice
%A Shan Li
%A Song-Mei Liu
%A Hao-Wei Fu
%A Jian-Zhong Huang
%A Qing-Yao Shu
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 8
%P 620-629
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700414

T1 - High-resolution melting-based TILLING of γ ray-induced mutations in rice
A1 - Shan Li
A1 - Song-Mei Liu
A1 - Hao-Wei Fu
A1 - Jian-Zhong Huang
A1 - Qing-Yao Shu
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 8
SP - 620
EP - 629
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700414

targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics strategy for the high-throughput screening of induced mutations. γ; radiation, which often induces both insertion/deletion (indel) and point mutations, has been widely used in mutation induction and crop breeding. The present study aimed to develop a simple, high-throughput TILLING system for screening γ; ray-induced mutations using high-resolution melting (HRM) analysis. Pooled rice (Oryza sativa) samples mixed at a 1:7 ratio of indel mutant to wild-type DNA could be distinguished from the wild-type controls by HRM analysis. Thus, an HRM-TILLING system that analyzes pooled samples of four M2 plants is recommended for screening γ; ray-induced mutants in rice. For demonstration, a γ; ray-mutagenized M2 rice population (n=4560) was screened for mutations in two genes, OsLCT1 and SPDT, using this HRM-TILLING system. Mutations including one single nucleotide substitution (G→A) and one single nucleotide insertion (A) were identified in OsLCT1, and one trinucleotide (TTC) deletion was identified in SPDT. These mutants can be used in rice breeding and genetic studies, and the findings are of importance for the application of γ; ray mutagenesis to the breeding of rice and other seed crops.


目的:建立适用于筛选伽马射线诱发突变的、基于高分辨率熔解曲线(high-resolution melting,HRM)技术的高通量定向诱导基因组局部突变技术(Targeting Induced Local Lesions IN Genomes,TILLING)体系.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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