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Received: 2020-10-10

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Huiming WANG


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.5 P.410-420


Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro

Author(s):  Baixiang WANG, Jiakang YANG, Lijie FAN, Yu WANG, Chenqiu ZHANG, Huiming WANG

Affiliation(s):  The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China; more

Corresponding email(s):   whmwhm@zju.edu.cn

Key Words:  Benidipine, Osteoblast, Osteogenesis, MC3T3-E1

Baixiang WANG, Jiakang YANG, Lijie FAN, Yu WANG, Chenqiu ZHANG, Huiming WANG. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro[J]. Journal of Zhejiang University Science B, 2021, 22(5): 410-420.

@article{title="Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro",
author="Baixiang WANG, Jiakang YANG, Lijie FAN, Yu WANG, Chenqiu ZHANG, Huiming WANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro
%A Baixiang WANG
%A Jiakang YANG
%A Lijie FAN
%A Chenqiu ZHANG
%A Huiming WANG
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 5
%P 410-420
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000628

T1 - Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro
A1 - Baixiang WANG
A1 - Jiakang YANG
A1 - Lijie FAN
A1 - Yu WANG
A1 - Chenqiu ZHANG
A1 - Huiming WANG
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 5
SP - 410
EP - 420
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000628

Hypertension is a prevalent systemic disease in the elderly, who can suffer from several pathological skeletal conditions simultaneously, including osteoporosis. benidipine (BD), which is widely used to treat hypertension, has been proved to have a beneficial effect on bone metabolism. In order to confirm the osteogenic effects of BD, we investigated its osteogenic function using mouse MC3T3-E1 preosteoblast cells in vitro. The proliferative ability of MC3T3-E1 cells was significantly associated with the concentration of BD, as measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cell cycle assay. With BD treatment, the osteogenic differentiation and maturation of MC3T3-E1 cells were increased, as established by the alkaline phosphatase (ALP) activity test, matrix mineralized nodules formation, osteogenic genetic test, and protein expression analyses. Moreover, our data showed that the BMP2/Smad pathway could be the partial mechanism for the promotion of osteogenesis by BD, while BD might suppress the possible function of osteoclasts through the OPG/RANKL/RANK (receptor activator of nuclear factor-κB (NF-κB)) pathway. The hypothesis that BD bears a considerable potential in further research on its dual therapeutic effect on hypertensive patients with poor skeletal conditions was proved within the limitations of the present study.




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