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On-line Access: 2021-05-07

Received: 2020-10-10

Revision Accepted: 2021-01-08

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Huiming WANG

https://orcid.org/0000-0002-1131-7455

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.5 P.410-420

http://doi.org/10.1631/jzus.B2000628


Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro


Author(s):  Baixiang WANG, Jiakang YANG, Lijie FAN, Yu WANG, Chenqiu ZHANG, Huiming WANG

Affiliation(s):  The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China; more

Corresponding email(s):   whmwhm@zju.edu.cn

Key Words:  Benidipine, Osteoblast, Osteogenesis, MC3T3-E1


Baixiang WANG, Jiakang YANG, Lijie FAN, Yu WANG, Chenqiu ZHANG, Huiming WANG. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro[J]. Journal of Zhejiang University Science B, 2021, 22(5): 410-420.

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author="Baixiang WANG, Jiakang YANG, Lijie FAN, Yu WANG, Chenqiu ZHANG, Huiming WANG",
journal="Journal of Zhejiang University Science B",
volume="22",
number="5",
pages="410-420",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000628"
}

%0 Journal Article
%T Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro
%A Baixiang WANG
%A Jiakang YANG
%A Lijie FAN
%A Yu WANG
%A Chenqiu ZHANG
%A Huiming WANG
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 5
%P 410-420
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000628

TY - JOUR
T1 - Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro
A1 - Baixiang WANG
A1 - Jiakang YANG
A1 - Lijie FAN
A1 - Yu WANG
A1 - Chenqiu ZHANG
A1 - Huiming WANG
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 5
SP - 410
EP - 420
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000628


Abstract: 
Hypertension is a prevalent systemic disease in the elderly, who can suffer from several pathological skeletal conditions simultaneously, including osteoporosis. benidipine (BD), which is widely used to treat hypertension, has been proved to have a beneficial effect on bone metabolism. In order to confirm the osteogenic effects of BD, we investigated its osteogenic function using mouse MC3T3-E1 preosteoblast cells in vitro. The proliferative ability of MC3T3-E1 cells was significantly associated with the concentration of BD, as measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cell cycle assay. With BD treatment, the osteogenic differentiation and maturation of MC3T3-E1 cells were increased, as established by the alkaline phosphatase (ALP) activity test, matrix mineralized nodules formation, osteogenic genetic test, and protein expression analyses. Moreover, our data showed that the BMP2/Smad pathway could be the partial mechanism for the promotion of osteogenesis by BD, while BD might suppress the possible function of osteoclasts through the OPG/RANKL/RANK (receptor activator of nuclear factor-κB (NF-κB)) pathway. The hypothesis that BD bears a considerable potential in further research on its dual therapeutic effect on hypertensive patients with poor skeletal conditions was proved within the limitations of the present study.

降压药贝尼地平在体外对小鼠MC3T3-E1细胞促成骨作用的初步研究

概要:高血压在老年人群中多发,而不良骨质情况例如骨质疏松症也多见于老年患者。贝尼地平(Benidipine,BD)作为广泛使用的抗高血压药,被发现具有一定的改善骨代谢的作用。为探究贝尼地平的促成骨作用,本实验选用了小鼠MC3T3-E1前成骨细胞进行体外实验。MTT及细胞周期试验显示M3CT3-E1细胞增殖能力受BD影响显著提升且具有浓度依赖性。碱性磷酸酶活力试验、基质矿化试验、成骨相关基因和蛋白表达的测定发现,BD可促进MC3T3-E1细胞的成骨向分化和成熟。此外,本项研究结果显示,BMP2/Smad通路在BD的促成骨效应中发挥作用,而OPG/RANKL/RANK通路的改变提示BD可能通过抑制破骨细胞的活动间接改善骨代谢。综上,在进一步研发治疗伴有不良骨质情况的高血压患者的双效药物方面,BD具有广阔的研究前景和应用潜力。

关键词:贝尼地平;成骨细胞;骨质疏松症;M3CT3-E1

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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