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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.9 P.767-773


Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628

Author(s):  Yefeng HU, Juan WANG, Jie XU, Zheng MA, Andreas BECHTHOLD, Xiaoping YU

Affiliation(s):  Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China; more

Corresponding email(s):   mazheng520@163.com

Key Words:  S. diastatochromogenes 1628, SAM, MetK, Toyocamycin, Tetraene macrolides

Yefeng HU, Juan WANG, Jie XU, Zheng MA, Andreas BECHTHOLD, Xiaoping YU. Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628[J]. Journal of Zhejiang University Science B, 2021, 22(9): 767-773.

@article{title="Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628",
author="Yefeng HU, Juan WANG, Jie XU, Zheng MA, Andreas BECHTHOLD, Xiaoping YU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628
%A Yefeng HU
%A Juan WANG
%A Jie XU
%A Zheng MA
%A Xiaoping YU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 9
%P 767-773
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100115

T1 - Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628
A1 - Yefeng HU
A1 - Juan WANG
A1 - Jie XU
A1 - Zheng MA
A1 - Andreas BECHTHOLD
A1 - Xiaoping YU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 9
SP - 767
EP - 773
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100115

Streptomyces are famous for their ability to synthesize a large number of bioactive compounds as secondary metabolites containing antibiotics, enzyme inhibitors, and other small molecules with potential physiological activity (Niu et al., 2016; Song et al., 2019; Yin et al., 2019). Secondary metabolites are produced by a multi-step reaction of a primary metabolite as a precursor (Liu et al., 2013; Li et al., 2021). Therefore, it is of great research significance to increase the overall synthesis level of antibiotics by increasing the amount of synthesis of precursors.


目的:考察S-腺苷甲硫氨酸(SAM)对淀粉酶产色链霉菌(Streptomyces diastatochromogenes)1628次级代谢产物产量的影响。
创新点:SAM是甲基的重要供体,可为次级代谢产物提供前体,同时也可以作为信号增强次级代谢产物合成基因的表达。因此,本研究首次探究了SAM与S. diastatochromogenes1628次级代谢产物合成的相关性。
方法:S. diastatochromogenes1628为对象,通过体外添加SAM以及在S. diastatochromogenes1628体内过表达、敲除和回补SAM编码基因metKsd,考察S. diastatochromogenes1628次级代谢产物产量的变化。
结论:由于渗透性差,外源添加SAM对S. diastatochromogenes1628合成次级代谢产物(丰加霉素和三种四烯大环内酯类抗生素:四霉素A、四霉素P和四烯菌素B)没有影响;敲除、回补和过表达基因metKsd证实增加胞内SAM可提高S. diastatochromogenes1628三种四烯大环内酯类抗生素的产量,且可促进四烯大环内酯类化合物合成关键基因的表达,而胞内SAM浓度的变化对丰加霉素的产量和及其关键基因的表达均无明显影响。


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