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On-line Access: 2022-04-11

Received: 2021-08-01

Revision Accepted: 2021-12-19

Crosschecked: 2022-04-19

Cited: 0

Clicked: 632

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Li-rong Shen

https://orcid.org/0000-0002-3197-9245

Cuiyun WU

https://orcid.org/0000-0002-0178-3524

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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.4 P.345-351

http://doi.org/10.1631/jzus.B2100673


Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region


Author(s):  Hao WEN, Xi JIANG, Wenqiang WANG, Minyu WU, Hongjin BAI, Cuiyun WU, Lirong SHEN

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   shenlirong@zju.edu.cn, 119900019@taru.edu.cn

Key Words:  Pear varieties, Chlorogenic acid, Transcriptome analysis, DEGs, WGCNA


Hao WEN, Xi JIANG, Wenqiang WANG, Minyu WU, Hongjin BAI, Cuiyun WU, Lirong SHEN. Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region[J]. Journal of Zhejiang University Science B, 2022, 23(4): 345-351.

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author="Hao WEN, Xi JIANG, Wenqiang WANG, Minyu WU, Hongjin BAI, Cuiyun WU, Lirong SHEN",
journal="Journal of Zhejiang University Science B",
volume="23",
number="4",
pages="345-351",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100673"
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%0 Journal Article
%T Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region
%A Hao WEN
%A Xi JIANG
%A Wenqiang WANG
%A Minyu WU
%A Hongjin BAI
%A Cuiyun WU
%A Lirong SHEN
%J Journal of Zhejiang University SCIENCE B
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%P 345-351
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100673

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T1 - Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region
A1 - Hao WEN
A1 - Xi JIANG
A1 - Wenqiang WANG
A1 - Minyu WU
A1 - Hongjin BAI
A1 - Cuiyun WU
A1 - Lirong SHEN
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 4
SP - 345
EP - 351
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2100673


Abstract: 
Pear is one of the main fruits with thousands of years of cultivation history in China. There are more than 2000 varieties of pear cultivars around the world, including more than 1200 varieties or cultivars in China (Legrand et al., 2016). Xinjiang Uygur Autonomous Region is an important pear production region in China with 30 of varieties or cultivars. Pyrus sinkiangensis is the most popular variety, which is mainly distributed in Xinjiang (Zhou et al., 2018). chlorogenic acid (CGA), p-coumaric acid, and arbutin are the main polyphenols in pear fruit, and their levels show great differences among different varieties (Li et al., 2014). CGA is a potential chemo-preventive agent, which possesses many important bioactivities including antioxidant, diabetes attenuating, and anti-obesity (Wang et al., 2021). Therefore, the specific CGA content of a variety is considered the embodiment of the functional nutritional value of pears.

三种新疆梨果发育过程中与绿原酸生物合成相关候选基因的转录组比较分析

目的:通过转录组学分析挖掘与新疆梨果中绿原酸生物合成相关的功能基因,为未来新疆梨的功能性育种提供科学依据。
创新点:明确了在三种新疆梨果发育过程中,与绿原酸含量变化趋势和绿原酸生物合成相关的差异基因及关键限制性酶。
方法:在鸭梨、库尔勒香梨和圆黄等三种梨果的不同发育阶段采集果实样品,经液相色谱法分析不同发育期果品的绿原酸含量和熊果苷含量差异,然后选择花后88、103和163天这三个绿原酸含量变化典型的时间点样品提取RNA,进行转录组测序,采取无参转录组学方法挖掘梨果中绿原酸生物合成途径的关键酶基因。
结论:绿原酸含量总体随着梨果的发育呈下降趋势。筛选得到10个与绿原酸生物合成相关的差异基因,分别为2个4CL基因、1个C4H基因、2个C3’H基因和5个HCT基因,其中绿原酸生物合成下游途径的C3’HHCT是关键的限速酶基因。

关键词:梨种;绿原酸,转录组分析;差异基因;加权基因共表达网络分析

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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