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On-line Access: 2022-04-11

Received: 2021-08-02

Revision Accepted: 2021-11-03

Crosschecked: 2022-04-19

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Citations:  Bibtex RefMan EndNote GB/T7714


Deping GUO


Jingze ZHANG


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.4 P.328-338


Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China

Author(s):  Zilan XIAO, Jianping DENG, Xiaojun ZHOU, Liyan ZHU, Xiaochan HE, Jingwu ZHENG, Deping GUO, Jingze ZHANG

Affiliation(s):  Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   jzzhang@zju.edu.cn, dpguo@zju.edu.cn

Key Words:  Zizania latifolia, Phylogeny, Pantoea ananatis, Multilocus analysis, Scanning electron microscopy, Species-specific primers

Zilan XIAO, Jianping DENG, Xiaojun ZHOU, Liyan ZHU, Xiaochan HE, Jingwu ZHENG, Deping GUO, Jingze ZHANG. Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China[J]. Journal of Zhejiang University Science B, 2022, 23(4): 328-338.

@article{title="Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China",
author="Zilan XIAO, Jianping DENG, Xiaojun ZHOU, Liyan ZHU, Xiaochan HE, Jingwu ZHENG, Deping GUO, Jingze ZHANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China
%A Zilan XIAO
%A Jianping DENG
%A Xiaojun ZHOU
%A Liyan ZHU
%A Xiaochan HE
%A Jingwu ZHENG
%A Deping GUO
%A Jingze ZHANG
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 4
%P 328-338
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100682

T1 - Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China
A1 - Zilan XIAO
A1 - Jianping DENG
A1 - Xiaojun ZHOU
A1 - Liyan ZHU
A1 - Xiaochan HE
A1 - Jingwu ZHENG
A1 - Deping GUO
A1 - Jingze ZHANG
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 4
SP - 328
EP - 338
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100682

The aquatic grass Zizania latifolia grows symbiotically with the fungus Ustilago esculenta producing swollen structures called Jiaobai, widely cultivated in China. A new disease of Z. latifolia was found in Zhejiang Province, China. Initial lesions appeared on the leaf sheaths or sometimes on the leaves near the leaf sheaths. The lesions extended along the axis of the leaf shoots and formed long brown to dark brown streaks from the leaf sheath to the leaf, causing sheath rot and death of entire leaves on young plants. The pathogen was isolated and identified as the bacterium Pantoea ananatis, based on 16S ribosomal RNA (rRNA) gene sequencing, multilocus sequence analysis (atpD (β-subunit of ATP synthase F1), gyrB (DNA gyrase subunit B), infB (translation initiation factor 2), and rpoB (β‍-subunit of RNA polymerase) genes), and pathogenicity tests. Ultrastructural observations using scanning electron microscopy revealed that the bacterial cells colonized the vascular tissues in leaf sheaths, forming biofilms on the inner surface of vessel walls, and extended between vessel elements via the perforated plates. To achieve efficient detection and diagnosis of P. ananatis, species-specific primer pairs were designed and validated by testing closely related and unrelated species and diseased tissues of Z. latifolia. This is the first report of bacterial sheath rot disease of Z. latifolia caused by P. ananatis in China.


方法:通过病原菌16S rRNA序列及结合多基因(atpDgyrBinfBrpoB)序列的系统发育分析,以及致病性试验结果,进行了病原菌鉴定;通过扫描电子显微镜,对病原菌与寄主互作的超微结构特征进行观察;分析了病原菌属内种间同源基因的碱基差异,设计出检测病原菌的专化性引物,并进行验证。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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