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On-line Access: 2023-11-14

Received: 2022-12-28

Revision Accepted: 2023-05-17

Crosschecked: 2023-11-15

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Fang LIU

https://orcid.org/0000-0002-7540-0370

Yuncheng LV

https://orcid.org/0000-0003-3575-194X

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.11 P.998-1013

http://doi.org/10.1631/jzus.B2200682


Sortilin-induced lipid accumulation and atherogenesis are suppressed by HNF1b SUMOylation promoted by flavone of Polygonatum odoratum


Author(s):  Fang LIU, Shirui CHEN, Xinyue MING, Huijuan LI, Zhaoming ZENG, Yuncheng LV

Affiliation(s):  Guangxi Key Laboratory of Diabetic Systems Medicine & Institute of Basic Medical Sciences, Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541199, China; more

Corresponding email(s):   anthony0723@163.com, zzhg88@163.com

Key Words:  Atherosclerosis, Lipid accumulation, Hepatocyte nuclear factor 1β, (HNF1b), Flavone of Polygonatum odoratum, SUMOylation


Fang LIU, Shirui CHEN, Xinyue MING, Huijuan LI, Zhaoming ZENG, Yuncheng LV. Sortilin-induced lipid accumulation and atherogenesis are suppressed by HNF1b SUMOylation promoted by flavone of Polygonatum odoratum[J]. Journal of Zhejiang University Science B, 2023, 24(11): 998-1013.

@article{title="Sortilin-induced lipid accumulation and atherogenesis are suppressed by HNF1b SUMOylation promoted by flavone of Polygonatum odoratum",
author="Fang LIU, Shirui CHEN, Xinyue MING, Huijuan LI, Zhaoming ZENG, Yuncheng LV",
journal="Journal of Zhejiang University Science B",
volume="24",
number="11",
pages="998-1013",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200682"
}

%0 Journal Article
%T Sortilin-induced lipid accumulation and atherogenesis are suppressed by HNF1b SUMOylation promoted by flavone of Polygonatum odoratum
%A Fang LIU
%A Shirui CHEN
%A Xinyue MING
%A Huijuan LI
%A Zhaoming ZENG
%A Yuncheng LV
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 11
%P 998-1013
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200682

TY - JOUR
T1 - Sortilin-induced lipid accumulation and atherogenesis are suppressed by HNF1b SUMOylation promoted by flavone of Polygonatum odoratum
A1 - Fang LIU
A1 - Shirui CHEN
A1 - Xinyue MING
A1 - Huijuan LI
A1 - Zhaoming ZENG
A1 - Yuncheng LV
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 11
SP - 998
EP - 1013
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200682


Abstract: 
This study aims to investigate the impact of hepatocyte nuclear factor 1β; (HNF1b) on macrophage sortilin-mediated lipid metabolism and aortic atherosclerosis and explore the role of the flavone of Polygonatum odoratum (PAOA-flavone)-promoted small ubiquitin-related modifier (SUMO) modification in the atheroprotective efficacy of HNF1b. HNF1b was predicted to be a transcriptional regulator of sortilin expression via bioinformatics, dual-luciferase reporter gene assay, and chromatin immunoprecipitation. HNF1b overexpression decreased sortilin expression and cellular lipid contents in THP-1 macrophages, leading to a depression in atherosclerotic plaque formation in low-density lipoprotein (LDL) receptor-deficient (LDLR-/-) mice. Multiple SUMO1-modified sites were identified on the HNF1b protein and co-immunoprecipitation confirmed its SUMO1 modification. The SUMOylation of HNF1b protein enhanced the HNF1b-inhibited effect on sortilin expression and reduced lipid contents in macrophages. PAOA-flavone treatment promoted SUMO-activating enzyme subunit 1 (SAE1) expression and SAE1-catalyzed SUMOylation of the HNF1b protein, which prevented sortilin-mediated lipid accumulation in macrophages and the formation of atherosclerotic plaques in apolipoprotein E-deficient (ApoE-/-) mice. Interference with SAE1 abrogated the improvement in lipid metabolism in macrophage cells and atheroprotective efficacy in vivo upon PAOA-flavone administration. In summary, HNF1b transcriptionally suppressed sortilin expression and macrophage lipid accumulation to inhibit aortic lipid deposition and the development of atherosclerosis. This anti-atherosclerotic effect was enhanced by PAOA-flavone-facilitated, SAE1-catalyzed SUMOylation of the HNF1b protein.

玉竹黄酮通过促进HNF1b蛋白的SUMO化修饰抑制分拣蛋白(sortilin)介导的脂质积累及动脉粥样硬化

刘芳1,陈诗芮1,明新月1,李慧娟1,曾昭明2,吕运成1
1广西糖尿病系统医学重点实验室&桂林医学院基础医学院基础医学研究所,中国桂林市,541199
2湖南明舜制药有限公司,中国邵东市,422800
摘要:本研究旨在探究肝细胞核因子1β(HNF1b)对巨噬细胞分拣蛋白(sortilin)介导的脂质代谢和主动脉粥样硬化的影响,以及玉竹(Polygonatum odoratum)黄酮在促进HNF1b的小泛素蛋白(SUMO)化修饰在动脉粥样硬化保护功效中的作用。通过生物信息学、双荧光素酶报告基因分析和染色质免疫共沉淀试验预测了HNF1b是sortilin表达的转录调控因子。HNF1b过表达降低了人髓系白血病单核(THP-1)巨噬细胞中sortilin的表达和细胞内脂质含量,从而抑制了低密度脂蛋白受体基因敲除(LDLR-/-)小鼠的动脉粥样斑块形成。在HNF1b蛋白上鉴定出多个SUMO1修饰位点,并通过免疫共沉淀证实存在SUMO1修饰。HNF1b蛋白的SUMO化修饰增强了HNF1b对sortilin表达的抑制作用,并降低了巨噬细胞中的脂质含量。玉竹黄酮处理促进了SUMO活化酶E1(SAE1)的表达和SAE1催化下对HNF1b蛋白的SUMO化修饰,从而阻止了巨噬细胞中sortilin介导的脂质积累和载脂蛋白E基因敲除(ApoE-/-)小鼠的动脉粥样斑块形成,而对SAE1的干扰使得巨噬细胞内脂质代谢的改善和玉竹黄酮治疗对体内抗动脉粥样硬化效果消失。因此,HNF1b通过转录抑制sortilin表达和巨噬细胞内脂质积累,抑制主动脉脂质沉积和动脉粥样硬化的发展,玉竹黄酮促进SAE1催化的HNF1b蛋白SUMO化修饰增强了其抗动脉粥样硬化效应。

关键词:动脉粥样硬化;脂质蓄积;HNF1b;玉竹黄酮;SUMO化修饰

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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