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Journal of Zhejiang University SCIENCE B 1998 Vol.-1 No.-1 P.

http://doi.org/10.1631/jzus.B2300738


Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor tu from Bacillus paramycoides


Author(s):  Pei LIU, Haiyu LONG, Shuai HE, Han CHENG, Erdong LI, Siyu CHENG, Mengdi LIANG, Zhengwei LIU, Zhen GUO, Hao SHI

Affiliation(s):  Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, P. R. China; more

Corresponding email(s):   liupeiouc@126.com

Key Words:  Selenium nanoparticles (SeNPs), Bacillus paramycoides, Elongation Factor Tu (EF-Tu), Selenite reduction, Green synthesis mechanism


Pei LIU, Haiyu LONG, Shuai HE, Han CHENG, Erdong LI, Siyu CHENG, Mengdi LIANG, Zhengwei LIU, Zhen GUO, Hao SHI. Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor tu from Bacillus paramycoides[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .

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Abstract: 
selenium nanoparticles (SeNPs) have been widely investigated and confirmed to have potential applications in various fields due to their unique characteristics. Therefore, the development of efficient and environmentally friendly synthesis methods for SeNPs is of great significance. The Bacillus paramycoides 24522 strain, preserved in our laboratory, has been proven to reduce selenite to SeNPs within 24 h, yielding highly stable and dispersible SeNPs with an average diameter of 150 nm (ranging from 100 nm to 200 nm) and a zeta potential of -29.9 mV. This study proposes an innovative and eco-friendly mechanism for synthesizing SeNPs through utilizing the intracellular protein elongation Factor Tu (EF-Tu), which was isolated from B. paramycoides 24522. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) analyses were used to confirm the identification of EF-Tu, which has a molecular mass of approximately 43 kDa and 395 amino acids. Quantitative real-time PCR (qPCR) revealed an 8.9-fold increase in the mRNA expression level of EF-Tu, further supporting its involvement in selenite reduction. Transmission electron microscopy (TEM) imaging of B. paramycoides 24522 indicated the intracellular location of SeNPs, suggesting a possible formation mechanism: small SeNPs are initially produced within the cell as seeds that interact with EF-Tu and subsequently undergo a maturation process, resulting in the formation of SeNPs, which are finally released into the medium through cell lysis. This innovative green synthesis mechanism is a promising avenue for the production of SeNPs and contributes to the advancement of environmentally friendly nanomaterial synthesis.

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