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Abstract: The 4th instar larvae of the silkworm, bombyx mori L, when treated with anti-juvenile hormone (Jinlu) had its larval period extended by 2 days and the total larval period shortened by about 4 days. The conversion ratio of tetramolters into trimolters was 100%. But anti-juvenile hormone administration to the 5th instar larvae lengthened the silkworm age by one day. When anti-juvenile hormone was administered, we could find many neurosecretory granules of the brain transferred to the cells of the corpora allata, but there was little endoplasmic reticulum. In the prothoracic gland, the micropile edge was clear and there were large nucleoli, mitochondria and endoplasmic reticulum. This study was carried out to show that anti-JH compound inhibits the secretion of Juvenile hormone in silkworm bombyx mori L. The investigation revealed that the anti-juvenile hormone inhibited the secretion of corpora allata and initiated the activity of the prothoracic gland.

[1] Akai, H., Kimura, K., Kiuchi, et al., 1984. Effects of anti-juvenoid treatment on cocoon and cocoon filaments in Bombyx mori. J. Seric. Sci. Jpn., 53(6):545-546.

[2] Akai, H., Kiuchi, M., Kimura, K., 1986. Effects of anti-JH treatment on the size and fine structure of the cocoon filament of Bombyx mori. J. Seric. Sci. Jpn., 55(5):388-396.

[3] Akai H., 1989. Supresive effects of an imidazole derivative KK-42 on JH levels in haemolymph of Bombyx mori. J. Seric. Sci. Jpn., 58(1):73-74.

[4] Bowers W. S., Ohta, T., Cleere, J. S., et al., 1976. Discovery of insect anti-juvenile hormone in plants. Science, 193(4253):542-547.

[5] Gibert LI, Rybczynski R, Tobe S., 1996. Endocrine cascade in insect metamorphosis. In: Gilbert LI, Tata J., Atkison P. (eds): Metamorphosis: Post-Embryonic Reprogramming of Gene Expression in Amphibian and Insect Cells. Academic Press, San Diego, p.59-107.

[6] Kiguchi, K., Mori, T., Akai, H., 1984. Effects of anti-juvenile hormone ETB on the development and metamorphosis of the silkworm Bombyx mori. J. Insect Physiol., 30(6):499-506.

[7] Kiuchi, M., Kimura, K., Akai, H., 1985. Induction of trimolters from a tetramolter strain of Bombyx mori by anti-juvenile hormone treatment. J. Seric. Sci. Jpn., 54(1):77-81.

[8] Lu Xuefang, Li Rongqi, 1987. Study on the trimolter induction of SM-1 to produce superior silk filament. J. of Sericultural Science, 13(2):71-75.

[9] Miao Yungen, Yu Shengwen, Ren Xiaomi, 1996. Mechanism of anti-JH and molting hormone to silkworm Bombyx mori. J. of Zhejiang Agricultural University, 22(4):345-348(in Chinese, with English abstract).

[10] Quistad, G. B., Cerf, D. C., Schooley, D.A., Staal, G. B., 1981. Fluoromevalonate acts as an inhibitor of insect juvenile hormone biosynthesis. Nature, 289(5794):176-77.

[11] Riddiford LM, 1994. Cellular and molecular actions of juvenile hormone: generalconsiderations and premetamorphic actions. Adv Insect Physiol., 24:213-274.

[12] Sehnal F., 1989. Hormone role of ecdysteroids in insect larvae and during metamorphosis. In: Koolman J. (ed), Ecdysone: From Chemistry to Mode of Action, Georg Thieme Stuttgart, p.271-278.

[13] Sato, T., 1968. A modified method for lead staining of thin sections. J. Electron Micr., 17:157-159.

[14] Staal G. B., 1986. Anti-juvenile hormone agents. Ann. Rev. Entomol., 31:391-429.

[15] Williams C. M., 1986. The present status of the brain hormone. In: Beament J. W. L. And Treherne J. E. (ed), Insect and physiology, Oviver & Boyd, Edinburgh and London, p.133-139.