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Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats
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CAI Wei, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na. Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats[J]. Journal of Zhejiang University Science B, 2005, 6(7): 622-625.
@article{title="Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats",
author="CAI Wei, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na",
journal="Journal of Zhejiang University Science B",
volume="6",
number="7",
pages="622-625",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0622"
}
%0 Journal Article
%T Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats
%A CAI Wei
%A CHEN Jun-zhu
%A RUAN Li-ming
%A WANG Yi-na
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 7
%P 622-625
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0622
TY - JOUR
T1 - Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats
A1 - CAI Wei
A1 - CHEN Jun-zhu
A1 - RUAN Li-ming
A1 - WANG Yi-na
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 7
SP - 622
EP - 625
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0622
Abstract: Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (Myo[Ca2+]) and activity of sarcoplosnic Ca2+-ATPase (SRCa2+-ATPase) in adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5 mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnI. CK-MB was detected by monoclonal antibody, Myo[Ca2+] was detected by fluorescent spectrophotometry and the activity of SRCa2+-ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnI and CK-MB, while at the same time decreased calcium concentration and increased SRCa2+-ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa2+-ATPase activity in cardiomyocytes.
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