Similar articles

Abstract: Several models of experimental ulcerative colitis have been reported previously. However, none of these models showed the optimum characteristics. Although dextran sulfate sodium-induced colitis results in inflammation resembling ulcerative colitis, an obvious obstacle is that dextran sulfate sodium is very expensive. The aim of this study was to develop an inexpensive model of colitis in rats. Sprague-Dawley rats were treated with 2% dextran sulfate sodium in drinking water for 3 d followed by an intracolonic administration of 30% ethanol. The administration of 2% dextran sulfate sodium followed by 30% ethanol induced significant weight loss, diarrhea and hematochezia in rats. Severe ulceration and inflammation of the distal part of rat colon were developed rapidly. Histological examination showed increased infiltration of polymorphonuclear leukocytes, lymphocytes and existence of cryptic abscesses and dysplasia. The model induced by dextran sulfate sodium at lower concentration followed by 30% ethanol is characterized by a clinical course, localization of the lesions and histopathological features similar to human ulcerative colitis and fulfills the criteria set out at the beginning of this study.

[1] Bamias, G., Nyce, M.R., de la Rue, S.A., Cominelli, F., 2005. New concepts in the pathophysiology of inflammatory bowel disease. Ann. Intern. Med., 143(12):895-904.

[2] Cooper, H.S., Murthy, S.N., Shah, R.S., Sedergran, D.J., 1993. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab. Invest., 69(2):238-249.

[3] Duchmann, R., Kaiser, I., Hermann, E., Mayet, W., Ewe, K., Meyer, B.K., 1995. Tolerance exists towards resident intestinal flora but is broken in active inflammatory bowel disease (IBD). Clin. Exp. Immunol., 102(3):448-455.

[4] Duchmann, R., Schmitt, E., Knolle, P., Meyer, K.H., Neurath, M.T., 1996. Tolerance towards resident intestinal flora in mice is abrogated in experimental colitis and restored by treatment with interleukin-10 or antibodies to interleukin-12. Eur. J. Immunol., 26(4):934-938.

[5] Egger, B., Bajaj-Elliott, M., MacDonald, T.T., Inglin, R., Eysselein, V.E., Buchler, M.W., 2000. Characterisation of acute murine dextran sodium sulphate colitis: cytokine profile and dose dependency. Digestion, 62(4):240-248.

[6] Faure, M., Moennoz, D., Montigon, F., Mettraux, C., Mercier, S., Schiffrin, E.J., 2003. Mucin production and composition is altered in dextran sulfate sodium-induced colitis in rats. Dig. Dis. Sci., 48(7):1366-1373.

[7] Gaudio, E., Taddei, G., Vetuschi, A., Sferra, R., Frieri, G., Ricciardi, G., 1999. Dextran sulfate sodium (DSS) colitis in rats: clinical, structural, and ultrastructural aspects. Dig. Dis. Sci., 44(7):1458-1475.

[8] Hibi, T., Ogata, H., Sakuraba, A., 2002. Animal models of inflammatory bowel disease. J. Gastroenterol., 37(6):409-417.

[9] Jiang, X.L., Cui, H.F., 2002. An analysis of 10218 ulcerative colitis cases in China. World J. Gastroenterol., 8(1):158-161.

[10] Lu, J., Wang, A., Ansari, S., Hershberg, R.M., McKay, D.M., 2003. Colonic bacterial superantigens evoke an inflammatory response and exaggerate disease in mice recovering from colitis. Gastroenterology, 125(6):1785-1795.

[11] Morris, G.P., Beck, P.L., Herridge, M.S., Depew, W.T., Szewczuk, M.R., Wallace, J.L., 1989. Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology, 96(3):795-803.

[12] Ni, J., Chen, S.F., Hollander, D., 1996. Effects of dextran sulphate sodium on intestinal epithelial cells and intestinal lymphocytes. Gut, 39(2):234-241.

[13] Ohtsuka, Y., Sanderson, I.R., 2003. Dextran sulfate sodium-induced inflammation is enhanced by intestinal epithelial cell chemokine expression in mice. Pediatr. Res., 53(1):143-147.

[14] Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, T., Inagaki, Y., Nakaya, R., 1990. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology, 98(3):694-702.

[15] Sands, B.E., 2007. Inflammatory bowel disease: past, present, and future. J. Gastroenterol., 42(1):16-25.

[16] Strober, W., 1985. Animal models of inflammatory bowel disease—an overview. Dig. Dis. Sci., 30(12 Suppl.):3S-10S.

[17] Vicario, M., Crespi, M., Franch, A., Amat, C., Pelegri, C., Moreto, M., 2005. Induction of colitis in young rats by dextran sulfate sodium. Dig. Dis. Sci., 50(1):143-150.

[18] Yu, Y., Sitaraman, S., Gewirtz, A.T., 2004. Intestinal epithelial cell regulation of mucosal inflammation. Immunol. Res., 29(1-3):55-68.