Similar articles

Abstract: A protein conjugate of streptomycin (streptomycin-bovine serum albumin (BSA) conjugate) was prepared and used as immunogen to produce monoclonal antibodies (MAb). One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentration (IC₅₀) value of 4.65 ng/ml and the IC₂₀ value of 0.21 ng/ml in phosphate buffered saline (PBS). At optimum conditions, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-based immunochromatographic assay (CGIA) were developed and applied to detect streptomycin residues in milk and swine urine samples. The developed ELISA showed that the minimum detection limit was 2.0 and 1.9 ng/ml for milk and swine urine samples, respectively, without obvious cross-reactivity to other tested antibiotics except dihydrostreptomycin which gave a 118.32% cross reaction value. Milk and swine urine samples spiked with streptomycin at 10, 50, 100 and 200 ng/ml were analyzed by the established ELISA. The mean recovery of streptomycin was from 81.9% to 105.5% and from 84.3% to 92.2% for milk and swine urine, respectively. The optimized CGIA showed that the minimum detection limit was 20.0 ng/ml for milk and swine urine samples. The results of spiked analysis and specific analysis demonstrate that the CGIA could be applicable for screening milk and swine urine samples for the presence of streptomycin residues on-site. The established ELISA and CGIA allow the rapid, low-cost, and sensitive determination of streptomycin residues in food samples.

[1] Abad, A., Moreno, M.J., Montoya, A., 1999. Development of monoclonal antibody-based immunoassays to the N-methylcarbamate pesticide carbofuran. J. Agric. Food Chem., 47(6):2475-2485.

[2] Abbasi, H., Hellenas, K.E., 1998. Modified determination of dihydrostreptomycin in kidney, muscle and milk by HPLC. Analyst, 123(12):2725-2727.

[3] Abuknesha, R.A., Luk, C., 2005. Enzyme immunoassays for the analysis of streptomycin in milk, serum and water: development and assessment of a polyclonal antiserum and assay procedures using novel streptomycin derivatives. Analyst, 130(6):964-970.

[4] Aga, D.S., Goldfish, R., Kulshrestha, P., 2003. Application of ELISA in determining the fate of tetracyclines in land-applied livestock wastes. Analyst, 128(6):658-662.

[5] Baxter, G., Ferguson, J.P., O'Connor, M.C., Elliott, C.T., 2001. Detection of streptomycin residues in whole milk using an optical immunobiosensor. J. Agric. Food Chem., 49(7):3204-3207.

[6] Ferguson, J.P., Baxter, G.A., McEvoy, J.D.G., Stead, S., Rawlings, E., Sharman, M., 2002. Detection of streptomycin and dihydrostreptomycin residues in milk, honey and meat samples using an optical biosensor. Analyst, 127(7):951-956.

[7] Grabar, K.C., Freeman, R.G., Hommer, M.B., Natan, M.J., 1995. Preparation and characterization of Au colloid monolayers. Anal. Chem., 67(4):735-743.

[8] Haasnoot, W., Stouten, P., Cazemier, G., Lommen, A., Nouws, J.F.M., Keukens, H.J., 1999. Immunochemical detection of aminoglycosides in milk and kidney. Analyst, 124(3):301-305.

[9] Haasnoot, W., Loomans, E., Cazemier, G., Dietrich, R., Verheijen, R., Bergwerff, A.A., Stephany, R.W., 2002. Direct versus competitive biosensor immunoassays for the detection of (dihydro)streptomycin residues in milk. Food Agric. Immunol., 14(1):15-27.

[10] Hammer, P., Kirchhoff, H., Hahn, G., 1993. Detection of streptomycins in raw milk by an antibody-capture immunoassay. Analyt. Chim. Acta, 275(1-2):313-316.

[11] Heering, W., Usleber, E., Dietrich, R., Martlbauer, E., 1998. Immunochemical screening for antimicrobial drug residues in commercial honey. Analyst, 123(12):2759-2762.

[12] Ingerslev, F., Halling-Sørensen, B., 2001. Biodegradability of metronidazole, olaquindox, and tylosin and formation of tylosin degradation products in aerobic soil-manure slurries. Ecotoxicol. Environ. Saf., 48(3):311-320.

[13] Jin, R.Y., Gui, W.J., Guo, Y.R., Wang, C.M., Wu, J.X., Zhu, G.N., 2008. Comparison of monoclonal antibody-based ELISA for triazophos between the indirect and direct formats. Food Agric. Immunol., 19(1):49-60.

[14] Mingeot-Leclercq, M., Glupczynski, Y., Tulkens, P.M., 1999. Aminoglycosides: activity and resistance. Antimicrob. Agents Chemother., 43(4):727-737.

[15] Molbak, K., Baggesen, D.L., Aarestrup, F.M., Ebbesen, J.M., Engberg, J., Frydendahl, K., Gerner-Smidt, P., Petersen, A.M., Wegener, H.C., 1999. An qutbreak of multidrug-resistant, quinolone-resistant salmonella enterica serotype typhimurium DT104. N. Engl. J. Med., 341(19):1420-1425.

[16] Pérez, S., McJury, B.E., Eichhorn, P., Aga, D.S., 2007. Determination of the antimicrobial growth promoter moenomycin-A in chicken litter. J. Chromatogr. A, 1175(2):234-241.

[17] Preu, M., Petz, M., 1999. Development and optimisation of a new derivatisation procedure for gas chromatographic-mass spectrometric analysis of dihydrostreptomcycin: comparison of multivariate and step-by-step optimisation procedures. J. Chromatogr. A, 840(1):81-91.

[18] Qian, G., Wang, L., Wu, Y., Zhang, Q., Sun, Q., Liu, Y., Liu, F., 2009. A monoclonal antibody-based sensitive enzyme-linked immunosorbent assay (ELISA) for the analysis of the organophosphorous pesticides chlorpyrifos-methyl in real samples. Food Chem., 117(2):364-370.

[19] Raab, G.M., 1983. Comparison of a logistic and a mass-action curve for radioimmunoassay data. Clin. Chem., 29(10):1757-1761.

[20] Samsonova, J.V., Bashkurov, M.L., Ivanova, N.L., Rubtsova, M.Y., Egorov, A.M., 2005. ELISA of streptomycin in buffer and milk: effect of reagents’ structure and analysis format on assay performance. Food Agric. Immunol., 16(1):47-57.

[21] Schnappinger, P., Usleber, E., Maertlbauer, E., Terplan, G., 1993. Enzyme immunoassay for the detection of streptomycin and dihydrostreptomycin in milk. Food Agric. Immunol., 5(2):67-73.

[22] Smith, K.E., Besser, J.M., Hedberg, C.W., Leano, F.T., Bender, J.B., Wicklund, J.H., Johnson, B.P., Moore, K.A., Osterholm, M.T., 1999. Quinolone-resistant campylobacter jejuni infections in minnesota, 1992-1998. N. Engl. J. Med., 340(20):1525-1532.

[23] Unusan, N., 2009. Occurrence of chloramphenicol, streptomycin and tetracycline residues in ultra-heat-treatment milk marketed in turkey. Int. J. Food Sci. Nutr., 60(5):359-364.

[24] Verheijen, R., Osswald, I.K., Dietrich, R., Haasnoot, W., 2000. Development of a one step strip test for the detection of (dihydro)streptomycin residues in raw milk. Food Agric. Immunol., 12(1):31-40.

[25] Wang, X., Li, K., Shi, D., Xiong, N., Jin, X., Yi, J., Bi, D., 2007. Development of an immunochromatographic lateral-flow test strip for rapid detection of sulfonamides in egg and chicken muscle. J. Agric. Food Chem., 55(6):2072-2078.

[26] Watanabe, H., Statake, A., Kido, Y., Tsuji, A., 2002. Monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic rapid assay for dihydrostreptomycin in milk. Analyt. Chim. Acta, 472(1-2):45-53.

[27] Wu, J.X., Yu, L., Li, L., Hu, J.Q., Zhou, J.Y., Zhou, X.P., 2007. Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens. Plant Biotechnol. J., 5(5):570-578.

[28] Yu, C., Wu, J.X., Zhou, X.P., 2005. Detection and subgrouping of cucumber mosaic virus isolates by TAS-ELISA and immunocapture RT-PCR. J. Virol. Methods, 123(2):155-161.

[29] Zhang, G.P., Wang, X.N., Yang, J.F., Yang, Y.Y., Xing, G.X., Li, Q.M., Zhao, D., Chai, S.J., Guo, J.Q., 2006. Development of an immunochromatographic lateral flow test strip for detection of β-adrenergic agonist clenbuterol residues. J. Immunol. Methods, 312(1-2):27-33.