CLC number: Q945.8
On-line Access: 2011-02-04
Received: 2010-07-21
Revision Accepted: 2010-11-05
Crosschecked: 2011-01-05
Cited: 10
Clicked: 7155
Qiu-lei Lang, Xiao-chuan Zhou, Xiao-lin Zhang, Rafal Drabek, Zhi-xiang Zuo, Yong-liang Ren, Tong-bin Li, Ji-shuang Chen, Xiao-lian Gao. Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection*#[J]. Journal of Zhejiang University Science B, 2011, 12(2): 116-125.
@article{title="Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection*#",
author="Qiu-lei Lang, Xiao-chuan Zhou, Xiao-lin Zhang, Rafal Drabek, Zhi-xiang Zuo, Yong-liang Ren, Tong-bin Li, Ji-shuang Chen, Xiao-lian Gao",
journal="Journal of Zhejiang University Science B",
volume="12",
number="2",
pages="116-125",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000278"
}
%0 Journal Article
%T Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection*#
%A Qiu-lei Lang
%A Xiao-chuan Zhou
%A Xiao-lin Zhang
%A Rafal Drabek
%A Zhi-xiang Zuo
%A Yong-liang Ren
%A Tong-bin Li
%A Ji-shuang Chen
%A Xiao-lian Gao
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 2
%P 116-125
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000278
TY - JOUR
T1 - Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection*#
A1 - Qiu-lei Lang
A1 - Xiao-chuan Zhou
A1 - Xiao-lin Zhang
A1 - Rafal Drabek
A1 - Zhi-xiang Zuo
A1 - Yong-liang Ren
A1 - Tong-bin Li
A1 - Ji-shuang Chen
A1 - Xiao-lian Gao
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 2
SP - 116
EP - 125
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000278
Abstract: A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate that developmental anomalies elicited by virus infection may be caused by more complex biological processes.
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