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3D printing ofhydroxyapatite/tricalcium phosphate scafold withhierarchical porous structure forbone regeneration
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LeiShao, QingGao, ChaoqiXie, JianzhongFu, MeixiangXiang, ZhenjieLiu, LiulinXiang, YongHe. 3D printing ofhydroxyapatite/tricalcium phosphate scafold withhierarchical porous structure forbone regeneration[J]. Journal of Zhejiang University Science D, 2020, 3(1): 30-39.
@article{title="3D printing ofhydroxyapatite/tricalcium phosphate scafold
withhierarchical porous structure forbone regeneration",
author="LeiShao, QingGao, ChaoqiXie, JianzhongFu, MeixiangXiang, ZhenjieLiu, LiulinXiang, YongHe",
journal="Journal of Zhejiang University Science D",
volume="3",
number="1",
pages="30-39",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00062-y"
}
%0 Journal Article
%T 3D printing ofhydroxyapatite/tricalcium phosphate scafold
withhierarchical porous structure forbone regeneration
%A LeiShao
%A QingGao
%A ChaoqiXie
%A JianzhongFu
%A MeixiangXiang
%A ZhenjieLiu
%A LiulinXiang
%A YongHe
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 1
%P 30-39
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00062-y
TY - JOUR
T1 - 3D printing ofhydroxyapatite/tricalcium phosphate scafold
withhierarchical porous structure forbone regeneration
A1 - LeiShao
A1 - QingGao
A1 - ChaoqiXie
A1 - JianzhongFu
A1 - MeixiangXiang
A1 - ZhenjieLiu
A1 - LiulinXiang
A1 - YongHe
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 1
SP - 30
EP - 39
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00062-y
Abstract: Three-dimensional (3D) bioprinting is a powerful approach that enables the fabrication of 3D tissue constructs that retain
complex biological functions. However, the dense hydrogel networks that form after the gelation of bioinks often restrict
the migration and proliferation of encapsulated cells. Herein, a sacrifcial microgel-laden bioink strategy was designed for
directly bioprinting constructs with mesoscale pore networks (MPNs) for enhancing nutrient delivery and cell growth. The
sacrifcial microgel-laden bioink, which contains cell/gelatin methacryloyl (GelMA) mixture and gelled gelatin microgel,
is frst thermo-crosslinked to fabricate temporary predesigned cell-laden constructs by extrusion bioprinting onto a cold
platform. Then, the construct is permanently stabilized through photo-crosslinking of GelMA. The MPNs inside the printed
constructs are formed after subsequent dissolution of the gelatin microgel. These MPNs allowed for efective oxygen/nutrient difusion, facilitating the generation of bioactive tissues. Specifcally, osteoblast and human umbilical vein endothelial
cells encapsulated in the bioprinted large-scale constructs (?1cm) with MPNs showed enhanced bioactivity during culture.
The 3D bioprinting strategy based on the sacrifcial microgel-laden bioink provided a facile method to facilitate formation
of complex tissue constructs with MPNs and set a foundation for future optimization of MPN-based tissue constructs with
applications in diverse areas of tissue engineering.
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