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On-line Access: 2024-04-29
Received: 2023-10-27
Revision Accepted: 2024-04-02
Crosschecked: 2024-04-29
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Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution
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Giuliana Grasso, Valentina Onesto, Stefania Forciniti, Eliana D’Amone, Francesco Colella, Lara Pierantoni, Valeria Famà, Giuseppe Gigli, Rui L. Reis, J. Miguel Oliveira & Loretta L. del Mercato. Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution[J]. Journal of Zhejiang University Science D, 2024, 7(3): 292-306.
@article{title="Highly sensitive ratiometric fluorescent fiber matrices for oxygen
sensing with micrometer spatial resolution",
author="Giuliana Grasso, Valentina Onesto, Stefania Forciniti, Eliana D’Amone, Francesco Colella, Lara Pierantoni, Valeria Famà, Giuseppe Gigli, Rui L. Reis, J. Miguel Oliveira & Loretta L. del Mercato",
journal="Journal of Zhejiang University Science D",
volume="7",
number="3",
pages="292-306",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00277-3"
}
%0 Journal Article
%T Highly sensitive ratiometric fluorescent fiber matrices for oxygen
sensing with micrometer spatial resolution
%A Giuliana Grasso
%A Valentina Onesto
%A Stefania Forciniti
%A Eliana D’Amone
%A Francesco Colella
%A Lara Pierantoni
%A Valeria Famà
%A Giuseppe Gigli
%A Rui L. Reis
%A J. Miguel Oliveira & Loretta L. del Mercato
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 3
%P 292-306
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00277-3
TY - JOUR
T1 - Highly sensitive ratiometric fluorescent fiber matrices for oxygen
sensing with micrometer spatial resolution
A1 - Giuliana Grasso
A1 - Valentina Onesto
A1 - Stefania Forciniti
A1 - Eliana D’Amone
A1 - Francesco Colella
A1 - Lara Pierantoni
A1 - Valeria Famà
A1 - Giuseppe Gigli
A1 - Rui L. Reis
A1 - J. Miguel Oliveira & Loretta L. del Mercato
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 3
SP - 292
EP - 306
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00277-3
Abstract: Oxygen (O2)-sensing matrices are promising tools for the live monitoring of extracellular O2 consumption levels in long-term
cell cultures. In this study, ratiometric O2-sensing membranes were prepared by electrospinning, an easy, low-cost, scalable,
and robust method for fabricating nanofibers. Poly(ε-caprolactone) and poly(dimethyl)siloxane polymers were blended with
tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) dichloride, which was used as the O2-sensing probe, and rhodamine B
isothiocyanate, which was used as the reference dye. The functionalized scaffolds were morphologically characterized by
scanning electron microscopy, and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy,
thermogravimetric analysis, and water contact angle measurement. The sensing capabilities were investigated by confocal
laser scanning microscopy, performing photobleaching, reversibility, and calibration curve studies toward different dissolved
O2 (DO) concentrations. Electrospun sensing nanofibers showed a high response to changes in DO concentrations in the
physiological-pathological range from 0.5% to 20% and good stability under ratiometric imaging. In addition, the sensing
systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines, namely
metastatic melanoma cell line SK-MEL2, breast cancer cell line MCF-7, and pancreatic ductal adenocarcinoma cell line
Panc-1, thus recreating a suitable biological environment in vitro. These O2-sensing biomaterials can potentially measure
alterations in cell metabolism caused by changes in ambient O2 content during drug testing/validation and tissue regeneration
processes.
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