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Bio-Design and Manufacturing  2023 Vol.6 No.4 P.451-463

http://doi.org/10.1007/s42242-023-00242-6


3D printing of personalized polylactic acid scaffold laden with GelMA/autologous auricle cartilage to promote ear reconstruction


Author(s):  Xingyu Gui, Zhiyu Peng, Ping Song, Li Chen, Xiujuan Xu, Hairui Li, Pei Tang, Yixi Wang, Zixuan Su, Qingquan Kong, Zhenyu Zhang, Zhengyong Li, Ying Cen, Changchun Zhou, Yujiang Fan & Xingdong Zhang

Affiliation(s):  National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China; more

Corresponding email(s):   changchunzhou@scu.edu.cn, yujiang.fan@163.com

Key Words:  Microtia, 3D printing, Polylactic acid (PLA) polymer scaffolds, Gelatin methacrylamide, Cartilage reconstruction


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Xingyu Gui, Zhiyu Peng, Ping Song, Li Chen, Xiujuan Xu, Hairui Li, Pei Tang, Yixi Wang, Zixuan Su, Qingquan Kong, Zhenyu Zhang, Zhengyong Li, Ying Cen, Changchun Zhou, Yujiang Fan & Xingdong Zhang . 3D printing of personalized polylactic acid scaffold laden with GelMA/autologous auricle cartilage to promote ear reconstruction[J]. Journal of Zhejiang University Science D, 2023, 6(4): 451-463.

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author="Xingyu Gui, Zhiyu Peng, Ping Song, Li Chen, Xiujuan Xu, Hairui Li, Pei Tang, Yixi Wang, Zixuan Su, Qingquan Kong, Zhenyu Zhang, Zhengyong Li, Ying Cen, Changchun Zhou, Yujiang Fan & Xingdong Zhang ",
journal="Journal of Zhejiang University Science D",
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number="4",
pages="451-463",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00242-6"
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%T 3D printing of personalized polylactic acid scaffold laden with GelMA/autologous auricle cartilage to promote ear reconstruction
%A Xingyu Gui
%A Zhiyu Peng
%A Ping Song
%A Li Chen
%A Xiujuan Xu
%A Hairui Li
%A Pei Tang
%A Yixi Wang
%A Zixuan Su
%A Qingquan Kong
%A Zhenyu Zhang
%A Zhengyong Li
%A Ying Cen
%A Changchun Zhou
%A Yujiang Fan & Xingdong Zhang
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A1 - Xingyu Gui
A1 - Zhiyu Peng
A1 - Ping Song
A1 - Li Chen
A1 - Xiujuan Xu
A1 - Hairui Li
A1 - Pei Tang
A1 - Yixi Wang
A1 - Zixuan Su
A1 - Qingquan Kong
A1 - Zhenyu Zhang
A1 - Zhengyong Li
A1 - Ying Cen
A1 - Changchun Zhou
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Abstract: 
At present, the clinical reconstruction of the auricle usually adopts the strategy of taking autologous costal cartilage. This method has great trauma to patients, poor plasticity and inaccurate shaping. Three-dimensional (3D) printing technology has made a great breakthrough in the clinical application of orthopedic implants. This study explored the combination of 3D printing and tissue engineering to precisely reconstruct the auricle. First, a polylactic acid (PLA) polymer scaffold with a precisely customized patient appearance was fabricated, and then auricle cartilage fragments were loaded into the 3D-printed porous PLA scaffold to promote auricle reconstruction. In vitro, gelatin methacrylamide (GelMA) hydrogels loaded with different sizes of rabbit ear cartilage fragments were studied to assess the regenerative activity of various autologous cartilage fragments. In vivo, rat ear cartilage fragments were placed in an accurately designed porous PLA polymer ear scaffold to promote auricle reconstruction. The results indicated that the chondrocytes in the cartilage fragments could maintain the morphological phenotype in vitro. After three months of implantation observation, it was conducive to promoting the subsequent regeneration of cartilage in vivo. The autologous cartilage fragments combined with 3D printing technology show promising potential in auricle reconstruction.

四川大学樊渝江周长春团队 | 3D打印个性化聚乳酸支架复合GelMA自体耳廓软骨碎片以促进耳廓重建

本研究论文聚焦于通过3D打印和组织工程相结合来精确重建耳廓。目前,小耳畸形导致的耳廓缺损在临床上通常采用自体肋软骨重建耳廓。这种方法对患者创伤大,可塑性困难,形状不精确。三维打印技术在个性化植入物的临床应用中取得了巨大突破。本研究采用3D打印和组织工程方法相结合以促进耳廓重建。首先,3D打印了精确定制的聚乳酸(PLA)耳廓支架,然后将耳廓软骨碎片加载到PLA支架中以重建耳廓。在体外,研究了负载不同大小兔耳软骨碎片的甲基丙烯酰化明胶(GelMA)水凝胶,以评估各种自体软骨碎片的再生活性。在体内,将大鼠耳软骨碎片放置在设计的多孔PLA耳廓支架中,以促进耳廓重建。结果表明,软骨碎片中的软骨细胞能够在体外维持形态表型。经过三个月植入观察,多孔支架复合软骨碎片有利于促进体内软骨的再生。由此,自体软骨碎片结合3D打印技术在耳廓重建中显示出良好的潜力。

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