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Abstract: The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200~260 g were divided into five groups and reared in 15 tanks with closed circuiting culture system. The animals were fed 5 diets: basal diet only (control) or supplemented of the basal diet with C. butyricum at doses of 10³ (CB1), 10⁵ (CB2), 10⁷ (CB3) or 10⁹ (CB4) CFU/g. Compared with the control, the serum phenoloxidase activity was significantly increased by the supplementation (P<0.05), acid phosphatases activity was increased significantly (P<0.05) at the doses of 10⁹ CFU/g. Serum lysozyme activity peaked at dose of 10⁷ CFU/g and in the skin mucus at dose of 10⁹ CFU/g. Immunoglobulin M level in the serum and skin mucus was increased except at dose of 10³ CFU/g (P<0.05). The growth at the dose of 10⁹ CFU/g was higher than that of the control (P<0.05). It is concluded that supplementation of C. butyricum can mediate the humoral immune responses and improve the growth performance in Miichthys miiuy.

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