Full Text:   <3637>

CLC number: R93

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2010-04-01

Cited: 13

Clicked: 8310

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.5 P.357-365

http://doi.org/10.1631/jzus.B0900397


Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide


Author(s):  Abdul Hamid Hafizah, Zakaria Zaiton, Amom Zulkhairi, Adenan Mohd Ilham, Megat Mohd Nordin Nor Anita, Abdullah Mahdy Zaleha

Affiliation(s):  Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Aziz, 50300 Kuala Lumpur, Malaysia, Department of Obstetrics and Gynecology, Universiti Kebangsaan Malaysia Medical Centre, Cheras, 56000 Kuala Lumpur, Malaysia, Department of Human Anatomy, Division of Physiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia, Forest Research Institute of Malaysia, Kepong, 52109 Selangor, Malaysia

Corresponding email(s):   zaitonukm@gmail.com

Key Words:  Piper sarmentosum, Human umbilical vein endothelial cells (HUVECs), Malondialdehyde, Oxidative stress, Antioxidant enzymes


Abdul Hamid Hafizah, Zakaria Zaiton, Amom Zulkhairi, Adenan Mohd Ilham, Megat Mohd Nordin Nor Anita, Abdullah Mahdy Zaleha. Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide[J]. Journal of Zhejiang University Science B, 2010, 11(5): 357-365.

@article{title="Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide",
author="Abdul Hamid Hafizah, Zakaria Zaiton, Amom Zulkhairi, Adenan Mohd Ilham, Megat Mohd Nordin Nor Anita, Abdullah Mahdy Zaleha",
journal="Journal of Zhejiang University Science B",
volume="11",
number="5",
pages="357-365",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900397"
}

%0 Journal Article
%T Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide
%A Abdul Hamid Hafizah
%A Zakaria Zaiton
%A Amom Zulkhairi
%A Adenan Mohd Ilham
%A Megat Mohd Nordin Nor Anita
%A Abdullah Mahdy Zaleha
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 5
%P 357-365
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900397

TY - JOUR
T1 - Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide
A1 - Abdul Hamid Hafizah
A1 - Zakaria Zaiton
A1 - Amom Zulkhairi
A1 - Adenan Mohd Ilham
A1 - Megat Mohd Nordin Nor Anita
A1 - Abdullah Mahdy Zaleha
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 5
SP - 357
EP - 365
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900397


Abstract: 
Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H2O2 (0–1000 µmol/L) and it was observed that 180 µmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H2O2-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 µg/ml) of three different extracts (aqueous, methanol and hexane) of PS. malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H2O2-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H2O2-induced HUVECs.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Aebi, H., 1984. Catalase in vitro. Methods Enzymol., 105:121-126.

[2]Ashokkumar, P., Sudhandiran, G., 2008. Protective role of luteolin on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis. Biomed. Pharmacother., 62(9):590-597.

[3]Benzie, I.F., Strain, J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay. Anal. Biochem., 239(1):70-76.

[4]Beyer, W.F.Jr., Fridovich, I., 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal. Biochem., 161(2):559-566.

[5]Bradford, M.M., 1976. A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2):248-254.

[6]Chanwitheesuk, A., Teerawutgulrag, A., Rakariyatham, N., 2005. Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chem., 92(3):491-497.

[7]Chung, J.E., Kim, S.Y., Jo, H.H., Hwang, S.J., Chae, B., Kwon, D.J., Lew, Y.O., Lim, Y.T., Kim, J.H., Kim, E.J., et al., 2008. Antioxidant effects of equol on bovine aorta endothelial cells. Biochem. Biophys. Res. Commun., 375(3):420-424.

[8]Cianchetti, S., Fiorentino, A.D., Colognato, R., Di Stefano, R., Franzoni, F., Pedrinelli, R., 2008. Anti-inflammatory and anti-oxidant properties of telmisartan in cultured human umbilical vein endothelial cells. Atherosclerosis, 198(1):22-28.

[9]Del Rio, D., Stewart, A.J., Pellegrini, N., 2005. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr. Metab., 15(4):316-328.

[10]Ee, G.C.L., Lim, C.M., Lim, C.K., Rahmani, M., Shaari, K., Bong, C.F.G., 2009. Alkaloids from Piper sarmentosum and Piper nigrum. Nat. Prod. Res., 23(15):1416-1423.

[11]Estany, S., Palacio, J.R., Barnadas, R., Sabes, M., Iborra, A., Martinez, P., 2007. Antioxidant activity of N-acetylcysteine, flavonoids and α-tocopherol on endometrial cells in culture. J. Reprod. Immunol., 75(1):1-10.

[12]Guan, S., Bao, Y.M., Jiang, B., An, L.J., 2006. Protective effects of protocatechuic acid from Alpinia oxyphylla on hydrogen peroxide-induced oxidative PC12 cell death. Eur. J. Pharmacol., 538(1-3):73-79.

[13]Halliwell, B., Gutteridge, J.M.C., 1989. Free Radicals in Biology and Medicine. Oxford University Press, Oxford, UK.

[14]Hou, Y.Z., Zhao, G.R., Yang, J., Yuan, Y.J., Zhu, G.G., Hiltunen, R., 2004. Protective effect of Ligusticum chuanxiong and Angelica sinensis on endothelial cell damage induced by hydrogen peroxide. Life Sci., 75(14):1775-1786.

[15]Hussain, K., Ismail, Z., Sadikun, A., Ibrahim, P., 2009a. Antioxidant, anti-TB activities, phenolic and amide contents of standardized extract of Piper sarmentosum Roxb. Nat. Prod. Res., 23(3):238-249.

[16]Hussain, K., Ismail, Z., Sadikun, A., Ibrahim, P., 2009b. Proximate analysis of different parts of Piper sarmentosum and quantification of total amides in various extracts. Phcog. Res., 2:113-119.

[17]Jaffe, E.A., Nachman, R.L., Becker, C.G., Minick, C.R., 1973. Culture of human umbilical endothelial cells derived from umbilical veins: identification by morphologic and immunologic criteria. J. Clin. Invest., 52(11):2745-2756.

[18]Lawrence, R.A., Burk, R.F., 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun., 71(4):952-958.

[19]Lin, R., Wang, W.R., Liu, J.T., Yang, G.D., Han, C.J., 2006. Protective efficacy of tanshinone IIA on human umbilical vein endothelial cell injured by hydrogen peroxide and its mechanism. J. Ethnopharmacol., 108(2):217-222.

[20]Lin, Y.L., Lee, T.F., Huang, Y.J., Huang, Y.T., 2006. Antiproliferative effect of salvianolic acid A on rat hepatic stellate cells. J. Pharm. Pharmacol., 58(7):933-939.

[21]Manju, V., Nalini, N., 2005. Chemopreventive efficacy of ginger, a naturally occurring anticarcinogenic during the initiation, post-initiation stages of 1,2-dimethylhyrazine-induced colon cancer. Clin. Chim. Acta, 358(1-2):60-70.

[22]Mates, J.M., 2000. Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology, 153(1-3):83-104.

[23]Miean, K.H., Mohamed, S., 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin and apigenin) content of edible tropical plants. J. Agric. Food Chem., 49(6):3106-3112.

[24]Ohkawa, H., Ohishi, N., Nagi, K., 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95(2):351.

[25]Perry, L.M., 1981. Medicinal Plants of East and Southeast Asia. MIT Press, Cambridge.

[26]Peungvicha, P., Thirawarapa, S., Temsiririrkkul, R., Watanabe, H., Prasain, J.K., Kadota, S., 1998. Hypoglycemic effect of the water extract of Piper sarmentosum in rats. J. Ethnopharmacol., 60(1):27-32.

[27]Pongboonrod, S., 1976. The Medicinal Plants of Thailand. Kasembanakit Press, Bangkok, Thailand.

[28]Pongmarutai, M., 1980. Studying Antidiabetic Action of Piper rostratum. MS Thesis, Mahidol University, Bangkok, Thailand.

[29]Renugadevi, J., Milton Praba, S., 2010. Cadmium-induced hepatoxicity in rats and the protective effect of naringenin. Exp. Toxicol. Pathol., 62(2):171-181.

[30]Ross, R., 1999. Atherosclerosis—an inflammatory disease. N. Engl. J. Med., 340(2):115-126.

[31]Seyoum, A., Asres, K., El-Fiky, F.K., 2006. Structure-radical scavenging activity relationships of flavonoids. Phytochemistry, 67(18):2058-2070.

[32]Spencer, J.P., Schroeter, H., Kuhnle, G., Srai, S.K., Tyrrell, R.M., Hahn, U., Rice-Evans, C., 2001. Epicatechin and its in vivo metabolite, 3′-O-methyl epicatechin, protect human fibroblasts from oxidative-stress-induced cell death involving caspase-3 activation. Biochem. J., 354(3):493-500.

[33]Stone, J.R., Yang, S., 2006. Hydrogen peroxide: a signaling messenger. Antioxid. Redox Signal., 8(3-4):243-270.

[34]Subramaniam, V., Adenan, M.I., Ahmad, A.R., Shahdan, R., 2003. Natural antioxidants: Piper sarmentosum (Kadok) and Morinda elliptica (Mengkudu). Mal. J. Nutr., 9(1):41-51.

[35]Takahashi, S., Abe, T., Gotoh, J., Fukuuchi, Y., 2002. Substrate-dependence of reduction of MTT: a tetrazolium dye differs in cultured astroglia and neurons. Neurochem. Int., 40(5):441-448.

[36]Upsani, C.D., Khera, A., Balaraman, R., 2001. Effect of lead and vitamin E, C or spiruline on malondialdehyde, conjugated dienes and hydroperoxides in rats. Indian J. Exp. Biol., 39(1):70-74.

[37]Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T.D., Mazur, M., Telser, J., 2007. Review: free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol., 39(1):44-84.

[38]van Acker, F.A.A., Schouten, O., Haenen, G.R.M.M., van der Vijgh, W.J.F., Bast, A., 2000. Flavonoids can replace α-tocopherol as an antioxidant. FEBS Lett., 473(2):145-148.

[39]Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D., 1998. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J. Agric. Food Chem., 46(10):4113-4117.

[40]Vinson, J.A., Dabbagh, Y.A., Serry, M.M., Jang, J., 1995. Plant flavonoids, especially tea flavonols are powerful antioxidants using an in vitro model for heart disease. J. Agric. Food Chem., 43(11):2800-2804.

[41]Wang, W.R., Lin, R., Peng, N., Han, C.J., 2006. The protective effects of tanshinone IIA on vascular endothelial cells injury induced by hydrogen peroxide. J. Clin. Med. Mater., 29:53-55.

[42]Wang, Y.K., Hong, Y.J., Huang, Z.Q., 2005. Protective effects of silybin on human umbilical vein endothelial cell injury induced by H2O2 in vitro. Vasc. Pharmacol., 43(4):198-206.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE