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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.1 P.40-46


Methotrexate ameliorates pristane-induced arthritis by decreasing IFN-γ and IL-17A expressions

Author(s):  Wei-kun Hou, Lie-su Meng, Fang Zheng, Yu-rong Wen, Wen-hua Zhu, Cong-shan Jiang, Xiao-jing He, Yan Zhou, She-min Lu

Affiliation(s):  Department of Genetics and Molecular Biology, School of Medicine, Xian Jiaotong University, Xian 710061, China, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xian Jiaotong University, Xian 710061, China, Department of Dermatology and Venereology, the First Affiliated Hospital, School of Medicine, Xian Jiaotong University, Xian 710061, China, Department of Epidemiology and Health Statistics, School of Public Health, Xian Jiaotong University, Xian 710061, China

Corresponding email(s):   shemin.lu@gmail.com

Key Words:  Methotrexate (MTX), Black seed oil (BSO), Pristane-induced arthritis (PIA), Interferon-γ, (IFN-γ, ), Interleukin-17A (IL-17A)

Wei-kun Hou, Lie-su Meng, Fang Zheng, Yu-rong Wen, Wen-hua Zhu, Cong-shan Jiang, Xiao-jing He, Yan Zhou, She-min Lu. Methotrexate ameliorates pristane-induced arthritis by decreasing IFN-γ and IL-17A expressions[J]. Journal of Zhejiang University Science B, 2011, 12(1): 40-46.

@article{title="Methotrexate ameliorates pristane-induced arthritis by decreasing IFN-γ and IL-17A expressions",
author="Wei-kun Hou, Lie-su Meng, Fang Zheng, Yu-rong Wen, Wen-hua Zhu, Cong-shan Jiang, Xiao-jing He, Yan Zhou, She-min Lu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Methotrexate ameliorates pristane-induced arthritis by decreasing IFN-γ and IL-17A expressions
%A Wei-kun Hou
%A Lie-su Meng
%A Fang Zheng
%A Yu-rong Wen
%A Wen-hua Zhu
%A Cong-shan Jiang
%A Xiao-jing He
%A Yan Zhou
%A She-min Lu
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 1
%P 40-46
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000078

T1 - Methotrexate ameliorates pristane-induced arthritis by decreasing IFN-γ and IL-17A expressions
A1 - Wei-kun Hou
A1 - Lie-su Meng
A1 - Fang Zheng
A1 - Yu-rong Wen
A1 - Wen-hua Zhu
A1 - Cong-shan Jiang
A1 - Xiao-jing He
A1 - Yan Zhou
A1 - She-min Lu
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 1
SP - 40
EP - 46
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000078

Objective: This study was carried out to test the effects of )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>methotrexate (MTX) and )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>black seed oil (BSO) on )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>pristane-induced arthritis (PIA) in rats. Methods: Inbred dark agouti (DA) rats were induced by a single subcutaneous injection of pristane, and then treated with MTX or BSO. Arthritis severity was evaluated macroscopically and microscopically. Plasma nitric oxide (NO) concentration was determined by the Griess method and cytokine mRNA expression in the spleen was detected by the real-time reverse transcription-polymerase chain reaction (RT-PCR). Results: The clinical arthritis severity was decreased after MTX treatment, while the BSO groups did not show significant changes compared with the disease group. The plasma NO level of the MTX group was significantly decreased compared with the disease group, but the BSO groups showed no difference from the disease group in plasma NO levels. The interferon-γ; (IFN-γ;) and interleukin-17A (IL-17A) mRNA expressions in the spleens were significantly decreased in the MTX group, but only showed a declining trend in the BSO groups compared with the disease group. Neither MTX nor BSO had an effect on the mRNA expressions of IL-4, transforming growth factor β (TGF-β), and tumor necrosis factor-α (TNF-α) in the spleen. Conclusions: MTX, but not BSO, can reduce the arthritis severity and decrease the mRNA expressions of IFN-γ and IL-17A in pristane-induced arthritis of rats.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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