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CLC number: R574.6

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Received: 2010-10-17

Revision Accepted: 2011-03-10

Crosschecked: 2011-08-16

Cited: 2

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.9 P.712-719


Hyaluronic acid as a rescue therapy for trinitrobenzene sulfonic acid-induced colitis through Cox-2 and PGE2 in a Toll-like receptor 4-dependent way

Author(s):  Huan Chen, Mahesh Mahaseth, Yan Zhang

Affiliation(s):  Department of Gastroenterology, West China Hospital of Sichuan University, Chengdu 610041, China

Corresponding email(s):   fengyixx@sohu.com

Key Words:  Trinitrobenzene sulfonic acid (TNBS) colitis, Therapy, Hyaluronic acid, Toll-like receptor

Huan Chen, Mahesh Mahaseth, Yan Zhang. Hyaluronic acid as a rescue therapy for trinitrobenzene sulfonic acid-induced colitis through Cox-2 and PGE2 in a Toll-like receptor 4-dependent way[J]. Journal of Zhejiang University Science B, 2011, 12(9): 712-719.

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author="Huan Chen, Mahesh Mahaseth, Yan Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Hyaluronic acid as a rescue therapy for trinitrobenzene sulfonic acid-induced colitis through Cox-2 and PGE2 in a Toll-like receptor 4-dependent way
%A Huan Chen
%A Mahesh Mahaseth
%A Yan Zhang
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 9
%P 712-719
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000362

T1 - Hyaluronic acid as a rescue therapy for trinitrobenzene sulfonic acid-induced colitis through Cox-2 and PGE2 in a Toll-like receptor 4-dependent way
A1 - Huan Chen
A1 - Mahesh Mahaseth
A1 - Yan Zhang
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 9
SP - 712
EP - 719
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000362

We hypothesized whether systemic administration of high-molecular-weight hyaluronic acid (HMW HA) could rescue trinitrobenzene sulfonic acid (TNBS)-induced colitis through toll-like receptor 4 (TLR4) signal. C3H/HeN mice and C3H/HeJ mice were used. Mice were divided into four groups: control, 50% ethanol treatment group, TNBS treatment group, and TNBS plus HA treatment group. The weight changes, clinical scores, macroscopic scores, and histological scores were recorded. Cyclooxygenase 2 (Cox-2) and prostaglandin E2 (PGE2) expressions were measured both in colons and peritoneal macrophages from these mice. HA was a rescue therapy for the colitis induced by TNBS only in C3H/HeN mice. The clinical score, macroscopic score, and histological score were much lower in C3H/HeN mice receiving TNBS plus HA treatment. Cox-2 and PGE2 expressions only increased in C3H/HeN mice. These Cox-2 expressing cells were macrophages. HA can also promote the production of Cox-2 and PGE2 in peritoneal macrophages from C3H/HeN mice. Our data demonstrated that HMW HA can rescue TNBS-induced colitis through inducing Cox-2 and PGE2 expressions in a TLR4-dependent way. Macrophages may be the effector cells of HMW HA.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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