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CLC number: R735.2

On-line Access: 2014-05-05

Received: 2013-06-23

Revision Accepted: 2013-11-11

Crosschecked: 2014-04-24

Cited: 2

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.5 P.500-506


Expression of three essential antioxidants of Helicobacter pylori in clinical isolates*

Author(s):  Yan-yan Shi, Mo Chen, Yue-xia Zhang, Jing Zhang, Shi-gang Ding

Affiliation(s):  . Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, China

Corresponding email(s):   dingshigang222@163.com

Key Words:  Antioxidant, Gastric cancer, Helicobacter pylori , Oxidative stress

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Yan-yan Shi, Mo Chen, Yue-xia Zhang, Jing Zhang, Shi-gang Ding. Expression of three essential antioxidants of Helicobacter pylori in clinical isolates[J]. Journal of Zhejiang University Science B, 2014, 15(5): 500-506.

@article{title="Expression of three essential antioxidants of Helicobacter pylori in clinical isolates",
author="Yan-yan Shi, Mo Chen, Yue-xia Zhang, Jing Zhang, Shi-gang Ding",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Expression of three essential antioxidants of Helicobacter pylori in clinical isolates
%A Yan-yan Shi
%A Mo Chen
%A Yue-xia Zhang
%A Jing Zhang
%A Shi-gang Ding
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 5
%P 500-506
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300171

T1 - Expression of three essential antioxidants of Helicobacter pylori in clinical isolates
A1 - Yan-yan Shi
A1 - Mo Chen
A1 - Yue-xia Zhang
A1 - Jing Zhang
A1 - Shi-gang Ding
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 5
SP - 500
EP - 506
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300171

Objective: Helicobacter pylori maintains long-term persistence in the host and combats oxidative stress via many antioxidant proteins, which are expected to be relevant to bacterial-associated gastric diseases. We aimed to investigate the expression of three essential antioxidants in H. pylori strains isolated from patients with different clinical outcomes. Methods: Forty H. pylori strains were isolated from endoscopic biopsy specimens of gastric mucosa from 13 patients with gastric cancer, 13 with peptic ulcer, and 14 with gastritis. The expression of thioredoxin 1 (Trx1), arginase (RocF), and alkyl hydroperoxide reductase (AhpC) in H. pylori was measured by real-time PCR. Comparisons among multiple sample sets were analyzed using a one-way ANOVA test. Pearson’s correlation test was used to assess relationships among multiple continuous variables. Results: Trx1 expression of H. pylori in gastric cancer and peptic ulcer tissues was higher than that in tissues with gastritis. RocF expression of H. pylori in gastric cancer tissues was higher than that in tissues exhibiting peptic ulcer and gastritis. However, we did not find any differences in AhpC expression in samples from patients with different clinical outcomes. The expression of Trx1 and RocF had a positive, linear correlation. The expression of Trx1 and AhpC had a positive correlation without a linear trend. We found no correlation between the expression of RocF and AhpC. Conclusions: Our observations indicate that the expression of Trx1 and RocF in H. pylori might be related to gastric carcinogenesis. In H. pylori, the expression of members of the antioxidant system may be correlated and relevant to gastric cancer.


研究目的:幽门螺杆菌(Helicobacter pylori)长期在宿主体内定植,并通过多种抗氧化蛋白对抗氧化应激,目前的研究认为该过程与其所致的胃粘膜疾病相关。我们拟临床研究不同胃粘膜疾病来源的H. pylori菌株中三个关键抗氧化物的表达。
创新要点:首次分析H. pylori临床分离菌株中氧化应激相关因子与胃粘膜疾病类型的关系,并对三种因子的表达水平进行相关性分析。突出临床病人感染H. pylori中氧化应激相关分子的表达特点,对研究H. pylori各氧化应激相关分子与临床胃粘膜疾病的关系,以及临床病人感染的H. pylori中各个氧化应激相关分子之间的关系具有重要的意义。
研究方法:在胃镜检查时,取患者胃粘膜组织进行H. pylori培养,共分离培养出40例临床菌株,其中13例来自胃癌患者,13例来自消化性溃疡患者,14例来自胃炎患者。用荧光实时定量聚合酶链式反应(PCR)的方法测定H. pylori临床分离菌株中硫氧还蛋白-1(Trx1)、精氨酸酶(RocF)以及烷基过氧化物酶(AhpC)的表达水平。多组样本间比较用单因素方差分析法,并用Pearson相关性分析法评价多组连续变量的相关性。
重要结论:H. pylori Trx1 mRNA表达水平在胃癌和消化性溃疡分离菌株中显著高于胃炎分离株,H. pylori RocF mRNA表达水平在胃癌患者分离菌株中显著高于胃炎和消化性溃疡分离株,我们未发现胃炎、消化性溃疡和胃癌患者分离的H. pylori菌株中AhpC的表达差异。H. pylori RocF的表达水平与Trx1呈显著线性正相关,AhpC的mRNA表达水平与Trx1呈非线性正相关,RocFAhpC的mRNA表达水平无相关性。结果表明,H. pylori Trx1RocF的表达水平可能与胃癌相关,在H. pylori菌株中,抗氧化系统的各个因子可能相互联系,在胃癌发病中发挥作用。


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[1] Alamuri, P., Maier, R.J., 2006. Methionine sulfoxide reductase in Helicobacter pylori: interaction with methionine-rich proteins and stress-induced expression. J Bacteriol, 188(16):5839-5850. 

[2] Baker, L.M., Raudonikiene, A., Hoffman, P.S., 2001. Essential thioredoxin-dependent peroxiredoxin system from Helicobacter pylori: genetic and kinetic characterization. J Bacteriol, 183(6):1961-1973. 

[3] Berndt, C., Lillig, C.H., Holmgren, A., 2008. Thioredoxins and glutaredoxins as facilitators of protein folding. Biochim Biophys Acta, 1783(4):641-650. 

[4] Chalker, A.F., Minehart, H.W., Hughes, N.J., 2001. Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis. J Bacteriol, 183(4):1259-1268. 

[5] Chen, L., Xie, Q.W., Nathan, C., 1998. Alkyl hydroperoxide reductase subunit C (AhpC) protects bacterial and human cells against reactive nitrogen intermediates. Mol Cell, 1(6):795-805. 

[6] Comtois, S.L., Gidley, M.D., Kelly, D.J., 2003. Role of the thioredoxin system and the thiol-peroxidases Tpx and Bcp in mediating resistance to oxidative and nitrosative stress in Helicobacter pyloriMicrobiology, 149(1):121-129. 

[7] Coussens, L.M., Werb, Z., 2002. Inflammation and cancer. Nature, 420(6917):860-867. 

[8] Croxen, M.A., Ernst, P.B., Hoffman, P.S., 2007. Antisense RNA modulation of alkyl hydroperoxide reductase levels in Helicobacter pylori correlates with organic peroxide toxicity but not infectivity. J Bacteriol, 189(9):3359-3368. 

[9] Gobert, A.P., McGee, D.J., Akhtar, M., 2001.  Helicobacter pylori arginase inhibits nitric oxide production by eukaryotic cells: a strategy for bacterial survival. PNAS, 98(24):13844-13849. 

[10] Handa, O., Naito, Y., Yoshikawa, T., 2010.  Helicobacter pylori: a ROS-inducing bacterial species in the stomach. Inflamm Res, 59(12):997-1003. 

[11] Hovey, J.G., Watson, E.L., Langford, M.L., 2007. Genetic microheterogeneity and phenotypic variation of Helicobacter pylori arginase in clinical isolates. BMC Microbiol, 7(1):26

[12] Huang, C.H., Chuang, M.H., Lo, W.L., 2011. Alkylhydroperoxide reductase of Helicobacter pylori as a biomarker for gastric patients with different pathological manifestations. Biochimie, 93(7):1115-1123. 

[13] McGee, D.J., Kumar, S., Viator, R.J., 2006.  Helicobacter pylori thioredoxin is an arginase chaperone and guardian against oxidative and nitrosative stresses. J Biol Chem, 281(6):3290-3296. 

[14] Olczak, A.A., Olson, J.W., Maier, R.J., 2002. Oxidative-stress resistance mutants of Helicobacter pyloriJ Bacteriol, 184(12):3186-3193. 

[15] Qu, W., Zhou, Y., Sun, Y., 2011. Identification of S-nitrosylation of proteins of Helicobacter pylori in response to nitric oxide stress. J Microbiol, 49(2):251-256. 

[16] Ritz, D., Beckwith, J., 2001. Roles of thiol-redox pathways in bacteria. Ann Rev Microbiol, 55(1):21-48. 

[17] Shen, R., Pan, S., Qi, S., 2010. Epigenetic repression of microRNA-129-2 leads to overexpression of SOX4 in gastric cancer. Biochem Biophys Res Commun, 394(4):1047-1052. 

[18] Shi, Y., Liu, L., Zhang, T., 2013. The involvement of Helicobacter pylori thioredoxin-1 in gastric carcinogenesis. J Med Microbiol, 62(Pt 8):1226-1234. 

[19] Wang, G., Alamuri, P., Maier, R.J., 2006. The diverse antioxidant systems of Helicobacter pyloriMol Microbiol, 61(4):847-860. 

[20] Windle, H.J., Fox, A., NıEidhin, D., 2000. The thioredoxin system of Helicobacter pyloriJ Biol Chem, 275(7):5081-5089. 

[21] Wroblewski, L.E., Peek, R.M., Wilson, K.T., 2010.  Helicobacter pylori and gastric cancer: factors that modulate disease risk. Clin Microbiol Rev, 23(4):713-739. 

[22] Zabaleta, J., McGee, D.J., Zea, A.H., 2004.  Helicobacter pylori arginase inhibits T cell proliferation and reduces the expression of the TCR ζ-chain (CD3ζ). J Immunol, 173(1):586-593. 

[23] Zhang, H.H., Cai, A.Z., Wei, X.M., 2013. Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 14(3):216-223. 

[24] Zhang, Y.N., Ding, S.G., Huang, L.H., 2011. Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis. J Zhejiang Univ Sci B (Biomed & Biotechnol), 12(10):820-827. 

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