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CLC number: R774.5

On-line Access: 2014-07-06

Received: 2013-07-19

Revision Accepted: 2013-11-07

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.7 P.661-669

http://doi.org/10.1631/jzus.B1300194


Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer*


Author(s):  Qiu-xia Jin1, Xin-ran Dong2, Jing-meng Chen3, Ke Yao2, Ya-lin Wu1,2

Affiliation(s):  1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   yalinw@zju.edu.cn

Key Words:  N-retinylidene-N-retinyl-ethanolamine (A2E), All-trans-retinal dimer, Lipofuscin, Organic solvents, Retinal pigment epithelial


Qiu-xia Jin, Xin-ran Dong, Jing-meng Chen, Ke Yao, Ya-lin Wu. Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer[J]. Journal of Zhejiang University Science B, 2014, 15(7): 661-669.

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author="Qiu-xia Jin, Xin-ran Dong, Jing-meng Chen, Ke Yao, Ya-lin Wu",
journal="Journal of Zhejiang University Science B",
volume="15",
number="7",
pages="661-669",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300194"
}

%0 Journal Article
%T Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer
%A Qiu-xia Jin
%A Xin-ran Dong
%A Jing-meng Chen
%A Ke Yao
%A Ya-lin Wu
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 7
%P 661-669
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300194

TY - JOUR
T1 - Effects of organic solvents on two retinal pigment epithelial lipofuscin fluorophores, A2E and all-trans-retinal dimer
A1 - Qiu-xia Jin
A1 - Xin-ran Dong
A1 - Jing-meng Chen
A1 - Ke Yao
A1 - Ya-lin Wu
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 7
SP - 661
EP - 669
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300194


Abstract: 
Gene and drug therapies are being developed to alleviate vision loss in patients with Stargardt’s disease and age-related macular degeneration (AMD). To evaluate the therapeutic effects of these treatments, organic solvents are routinely used to extract and quantify bisretinoid lipofuscin constituents, such as N-retinylidene-N-retinyl-ethanolamine (A2E) and all-trans-retinal dimer (ATR-dimer). By high-performance liquid chromatography (HPLC), we found that A2E and ATR-dimer were both altered by tetrahydrofuran (THF) and chloroform, but were stable in dimethyl sulfoxide (DMSO) or methanol (MeOH). In addition, cyclohexane and ethanol (EtOH) did not alter ATR-dimer, whereas an alteration of A2E occurred in EtOH. On the basis of these findings, we designed processes II–IV, generated by modifications of process I, a routine method to measure bisretinoid compounds in vivo. Extra amounts of either ATR-dimer or A2E in mouse eyecups were released by processes II–IV versus process I. Efforts to clarify the effects of organic solvents on lipofuscin pigments are important because such studies can guide the handling of these fluorophores in related experiments.

有机溶剂对两种视网膜色素上皮脂褐质荧光色素的影响

研究目的:减缓Stargardt和年龄相关性黄斑变性患者视力损失的基因和药物疗法正处于发展中,然而为了评价对实验小鼠的治疗效果,有机溶剂是获取及定量小鼠后眼罩脂褐质色素必需的载体。本工作报道了有机溶剂对N-亚视黄基-N-视黄基乙醇胺(A2E)和全反式视黄醛二聚体两种重要脂褐质色素的影响以及从体内获取和定量它们的优化方法。
创新要点:本文阐释了二甲亚砜、甲醇、氯仿、四氢呋喃、环己烷和乙醇对A2E和全反式视黄醛二聚体两种脂褐质色素的影响,并且优化了它们的体内获取及定量程序。
研究方法:采用高效液相色谱技术分析A2E和全反式视黄醛二聚体溶液以及C57BL/6小鼠后眼罩浸膏。
重要结论:氯仿和四氢呋喃能够改变A2E和全反式视黄醛二聚体,但是它们在二甲亚砜和甲醇中是稳定的;环己烷和乙醇不影响全反式视黄醛二聚体,然而A2E在乙醇中不稳定,在环己烷中溶解度很低。脂褐质元素体内获取及定量的优化方法能够释放出更多的全反式视黄醛二聚体和A2E。理解有机溶剂对脂褐质色素的影响将为正确使用这些色素提供洞察力,也可促进建立每个内源性色素测量的合理方法。

关键词:脂褐质;类视色素二聚体;有机溶剂;视网膜色素上皮;荧光色素

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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