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CLC number: R283; O65

On-line Access: 2014-11-04

Received: 2014-06-05

Revision Accepted: 2014-08-07

Crosschecked: 2014-10-11

Cited: 15

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.11 P.940-952

http://doi.org/10.1631/jzus.B1400158


Essential oil from Zanthoxylum bungeanum Maxim. and its main components used as transdermal penetration enhancers: a comparative study*


Author(s):  Yi Lan, Hui Li, Yan-yan Chen, Ye-wen Zhang, Na Liu, Qing Zhang, Qing Wu

Affiliation(s):  . School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China

Corresponding email(s):   qwu@vip.sina.com

Key Words:  Zanthoxylum bungeanum Maxim., Essential oil, Limonene, Fourier transform infrared (FTIR) spectroscopy, Penetration enhancer, HaCaT


Yi Lan, Hui Li, Yan-yan Chen, Ye-wen Zhang, Na Liu, Qing Zhang, Qing Wu. Essential oil from Zanthoxylum bungeanum Maxim. and its main components used as transdermal penetration enhancers: a comparative study[J]. Journal of Zhejiang University Science B, 2014, 15(11): 940-952.

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author="Yi Lan, Hui Li, Yan-yan Chen, Ye-wen Zhang, Na Liu, Qing Zhang, Qing Wu",
journal="Journal of Zhejiang University Science B",
volume="15",
number="11",
pages="940-952",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400158"
}

%0 Journal Article
%T Essential oil from Zanthoxylum bungeanum Maxim. and its main components used as transdermal penetration enhancers: a comparative study
%A Yi Lan
%A Hui Li
%A Yan-yan Chen
%A Ye-wen Zhang
%A Na Liu
%A Qing Zhang
%A Qing Wu
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 11
%P 940-952
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400158

TY - JOUR
T1 - Essential oil from Zanthoxylum bungeanum Maxim. and its main components used as transdermal penetration enhancers: a comparative study
A1 - Yi Lan
A1 - Hui Li
A1 - Yan-yan Chen
A1 - Ye-wen Zhang
A1 - Na Liu
A1 - Qing Zhang
A1 - Qing Wu
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 11
SP - 940
EP - 952
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400158


Abstract: 
Our previous studies had confirmed that the essential oil from Zanthoxylum bungeanum Maxim. (Z. bungeanum oil) could effectively enhance the percutaneous permeation of drug molecules as a natural transdermal penetration enhancer. The aim of the present study is to investigate and compare the skin penetration enhancement effect of Z. bungeanum oil and its main components on traditional Chinese medicine (TCM) active components. Toxicities of Z. bungeanum oil and three selected terpene compounds (terpinen-4-ol, 1,8-cineole, and limonene) in epidermal keratinocytes (haCaT) and dermal fibroblast (CCC-ESF-1) cell lines were measured using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Five model drugs in TCM external preparations, namely osthole (OT), tetramethylpyrazine (TMP), ferulic acid (FA), puerarin (PR), and geniposide (GP), which were selected based on their lipophilicity denoted by logK o/w, were tested using in vitro permeation studies in which vertical Franz diffusion cells and rat abdominal skin were employed. The secondary structure changes of skin stratum corneum (SC) and drug thermodynamic activities were investigated to understand their mechanisms of action using fourier transform infrared (FTIR) spectroscopy and saturation solubility studies, respectively. It was found that Z. bungeanum oil showed lower toxicities in both haCaT cells and CCC-ESF-1 cells compared with three terpene compounds used alone. The enhancement permeation capacities by all tested agents were in the following increasing order: terpinen-4-ol≈1,8-cineole<limonene<Z. bungeanum oil. The mechanisms of permeation enhancement suggested that these enhancers promoted the skin permeation of drugs mainly by affecting SC lipids. These results indicated that Z. bungeanum oil exhibited better performance in enhancing the skin permeation of active components in TCM preparations.

花椒挥发油与其主要成分经皮促透作用的比较研究

比较花椒挥发油与其主要成分作为经皮促透剂的皮肤细胞毒性,对系列不同油水分配系数药物促透作用特征及其作用机制。 选择系列不同油水分配系数中药活性成分简化并表征中药制剂中复杂有效成分,对比研究花椒挥发油与其主要成分的经皮促透作用特征差异。 利用表皮角质形成细胞(HaCaT)和真皮成纤维细胞(CCC-ESF-1)测定其皮肤细胞毒性,选择系列不同油水分配系数中药活性成分作为模型药物测定花椒挥发油与其主要成分的经皮促透作用特征差异,并在此基础上采用傅利叶变换红外光谱(FTIR)等研究其促透作用机制。 花椒挥发油相对于其单一组成成分具有更好的经皮促透效果。
花椒挥发油;柠檬烯;促透剂;表皮角质形成细胞(HaCaT);傅利叶变换红外光谱(FTIR)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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