Full Text:   <2935>

Summary:  <1617>

CLC number: R698

On-line Access: 2015-03-05

Received: 2014-07-13

Revision Accepted: 2014-10-31

Crosschecked: 2015-02-21

Cited: 10

Clicked: 5196

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Thawatchai Prabsattroo

http://orcid.org/0000-0001-6292-9040

Jintanaporn Wattanathorn

http://orcid.org/0000-0002-7383-2348

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.3 P.179-190

http://doi.org/10.1631/jzus.B1400197


Moringa oleifera extract enhances sexual performance in stressed rats


Author(s):  Thawatchai Prabsattroo, Jintanaporn Wattanathorn, Sitthichai Iamsaard, Pichet Somsapt, Opass Sritragool, Wipawee Thukhummee, Supaporn Muchimapura

Affiliation(s):  Graduate School and Department of Physiology (Neuroscience Program), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; more

Corresponding email(s):   backwert@gmail.com, jintanapornw@yahoo.com

Key Words:  Moringa oleifera, Sexual behaviors, Stress


Thawatchai Prabsattroo, Jintanaporn Wattanathorn, Sitthichai Iamsaard, Pichet Somsapt, Opass Sritragool, Wipawee Thukhummee, Supaporn Muchimapura. Moringa oleifera extract enhances sexual performance in stressed rats[J]. Journal of Zhejiang University Science B, 2015, 16(3): 179-190.

@article{title="Moringa oleifera extract enhances sexual performance in stressed rats",
author="Thawatchai Prabsattroo, Jintanaporn Wattanathorn, Sitthichai Iamsaard, Pichet Somsapt, Opass Sritragool, Wipawee Thukhummee, Supaporn Muchimapura",
journal="Journal of Zhejiang University Science B",
volume="16",
number="3",
pages="179-190",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400197"
}

%0 Journal Article
%T Moringa oleifera extract enhances sexual performance in stressed rats
%A Thawatchai Prabsattroo
%A Jintanaporn Wattanathorn
%A Sitthichai Iamsaard
%A Pichet Somsapt
%A Opass Sritragool
%A Wipawee Thukhummee
%A Supaporn Muchimapura
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 3
%P 179-190
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400197

TY - JOUR
T1 - Moringa oleifera extract enhances sexual performance in stressed rats
A1 - Thawatchai Prabsattroo
A1 - Jintanaporn Wattanathorn
A1 - Sitthichai Iamsaard
A1 - Pichet Somsapt
A1 - Opass Sritragool
A1 - Wipawee Thukhummee
A1 - Supaporn Muchimapura
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 3
SP - 179
EP - 190
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400197


Abstract: 
Aphrodisiacs are required to improve male sexual function under stressful conditions. Due to the effects of oxidative stress and dopamine on male sexual function, we hypothesized that Moringa oleifera leaves might improve male sexual dysfunction induced by stress. Therefore, the effects on various factors playing important roles in male sexual behavior, such as antioxidant effects, the suppression of monoamine and phosphodiesterase type 5 (PDE-5) activities, serum testosterone and corticosterone levels, and histomorphological changes in the testes, of a hydroethanolic extract of M. oleifera leaves were investigated. Various doses of extract including 10, 50, and 250 mg/kg body weight (BW) were given orally to male Wistar rats before exposure to 12 h-immobilization stress for 7 d. The results demonstrated that the extract showed both antioxidant and monoamine oxidase type B (MAO-B) suppression activities. At 7 d of treatment, the low dose of extract improved sexual performance in stress-exposed rats by decreasing intromission latency and increasing intromission frequency. It also suppressed PDE-5 activity, decreased serum corticosterone level, but increased serum testosterone, numbers of interstitial cells of Leydig and spermatozoa. The increased numbers of interstitial cells of Leydig and spermatozoa might have been due to the antioxidant effect of the extract. The increased sexual performance during the intromission phase might have been due to the suppression of MAO-B and PDE-5 activities and increased testosterone. Therefore, M. oleifera is a potential aphrodisiac, but further research concerning the precise underlying mechanisms is still needed.

辣木提取物对应激大鼠性能力具有增强作用

中文概要:
目的:探讨辣木叶提取物对改善应激条件下雄性性功能障碍的作用。
创新点:根据氧化应激和多巴胺对雄性性功能的影响,推测具有抗氧化和单胺调节功能的辣木叶能改善应激条件下的雄性性功能障碍。
方法:本文以雄性Wistar大鼠作为实验对象,通过对影响雄性性功能的各种因素进行测量及统计学分析,包括抗氧化性、B型单胺氧化酶(MAO-B)和5型磷酸二酯酶(PDE-5)活性、血清睾酮和皮质甾酮水平、睾丸内组织形态学变化。
结论:研究结果表明,辣木叶提取物作为一种抗氧化剂具有抑制MAO-B和PDE-5活性的作用,同时能降低血清皮质酮水平,提高血清睾酮水平,增加睾丸间质细胞和精子的数量。睾丸间质细胞和精子的数量的增加可能是由于辣木叶提取物的抗氧化作用,插入阶段性功能增强可能是由于MAO-B和PDE-5活性的抑制和睾酮水平的增加。因此,辣木可以作为一种潜在的壮阳药。

关键词:辣木;性功能;应激

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Ahmad, A., Rasheed, N., Gupta, P., et al., 2012. Novel Ocimumoside A and B as anti-stress agents: modulation of brain monoamines and antioxidant systems in chronic unpredictable stress model in rats. Phytomedicine, 19(7):639-647.

[2]Al-Mohaisen, M., Cardounel, A., Kalimi, M., 2000. Repeated immobilization stress increases total cytosolic glucocorticoid receptor in rat liver. Steroids, 65(1):8-15.

[3]Andersson, K.E., 2001. Pharmacology of penile erection. Pharmacol. Rev., 53(3):417-450.

[4]Aversa, A., Bruzziches, R., Francomano, D., et al., 2009. Testosterone and phosphodiesterase type-5 inhibitors: new strategy for preventing endothelial damage in internal and sexual medicine. Ther. Adv. Urol., 1(4):179-197.

[5]Baldwin, D.S., 2001. Depression and sexual dysfunction. Br. Med. Bull., 57(1):81-99.

[6]Basma, A.A., Zakaria, Z., Latha, L.Y., et al., 2011. Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L. Asian Pac. J. Trop. Med., 4(5):386-390.

[7]Bitgul, G., Tekmen, I., Keles, D., et al., 2013. Protective effects of resveratrol against chronic immobilization stress on testis. ISRN Urol., 2013:278720.

[8]Carrasco, G.A., van de Kar, L.D., 2003. Neuroendocrine pharmacology of stress. Eur. J. Pharmacol., 463(1-3):235-272.

[9]de Ancos, B., Sgroppo, S., Plaza, L., et al., 2002. Possible nutritional and health-related value promotion in orange juice preserved by high-pressure treatment. J. Sci. Food Agric., 82(8):790-796.

[10]de Young, L., Yu, D., Bateman, R.M., et al., 2004. Oxidative stress and antioxidant therapy: their impact in diabetes-associated erectile dysfunction. J. Androl., 25(5):830-836.

[11]Dhingra, D., Goyal, P.K., 2008. Evidences for the involvement of monoaminergic and GABAergic systems in antidepressant-like activity of Tinospora cordifolia in mice. Indian J. Pharm. Sci., 70(6):761-767.

[12]El-Shafey, H., Atteya, A., el-Magd, S.A., et al., 2006. Tianeptine can be effective in men with depression and erectile dysfunction. J. Sex. Med., 3(5):910-917.

[13]Fauci, A.S., Wiener, C.M., Brown, C., 2005. Harrison’s Principles of Internal Medicine, 16th Ed. McGraw-Hill, Medical Pub. Division.

[14]Ganguly, R., Guha, D., 2008. Alteration of brain monoamines & EEG wave pattern in rat model of Alzheimer’s disease & protection by Moringa oleifera. Indian J. Med. Res., 128(6):744-751.

[15]Gao, H.B., Shan, L.X., Monder, C., et al., 1996. Suppression of endogenous corticosterone levels in vivo increases the steroidogenic capacity of purified rat Leydig cells in vitro. Endocrinology, 137(5):1714-1718.

[16]Gauthaman, K., Adaikan, P.G., Prasad, R.N.V., 2002. Aphrodisiac properties of Tribulus terrestris extract (protodioscin) in normal and castrated rats. Life Sci., 71(12):1385-1396.

[17]Hamed, E., Meki, A., Gaafar, A., et al., 2003. Role of some vasoactive mediators in patients with erectile dysfunction: their relationship with angiotensin-converting enzyme and growth hormone. Int. J. Impot. Res., 15(6):418-425.

[18]Holt, A., Sharman, D.F., Baker, G.B., et al., 1997. A continuous spectrophotometric assay for monoamine oxidase and related enzymes in tissue homogenates. Anal. Biochem., 244(2):384-392.

[19]Hull, E.M., Du, J., Lorrain, D.S., et al., 1997. Testosterone, preoptic dopamine, and copulation in male rats. Brain Res. Bull., 44(4):327-333.

[20]Hull, E.M., Muschamp, J.W., Sato, S., 2004. Dopamine and serotonin: influences on male sexual behavior. Physiol. Behav., 83(2):291-307.

[21]Invernizzi, R., Pozzi, L., Garattini, S., et al., 1992. Tianeptine increases the extracellular concentrations of dopamine in the nucleus accumbens by a serotonin-independent mechanism. Neuropharmacology, 31(3):221-227.

[22]Kennedy, S.H., Dickens, S.E., Eisfeld, B.S., et al., 1999. Sexual dysfunction before antidepressant therapy in major depression. J. Affect. Disord., 56(2):201-208.

[23]Kothari, P., 2001. Common Sexual Problems, 3rd Ed. UBS Publishers Distributors (P), Ltd., New Delhi, India.

[24]Lewis, R.W., Fugl-Meyer, K.S., Bosch, R., et al., 2004. Epidemiology/risk factors of sexual dysfunction. J. Sex. Med., 1(1):35-39.

[25]Makonnen, E., Hunde, A., Damecha, G., 1997. Hypoglycaemic effect of Moringa stenopetala aqueous extract in rabbits. Phytother. Res., 11(2):147-148.

[26]Meston, C.M., Frohlich, P.F., 2000. The neurobiology of sexual function. Arch. Gen. Psychiatry, 57(11):1012-1030.

[27]Minhas, S., Jeremy, J.Y., Jones, R.W.A., et al., 2002. Oxygen free radicals and the penis. Expert Opin. Pharmacother., 3(7):889-897.

[28]Monera, T.G., Wolfe, A.R., Maponga, C.C., et al., 2008. Moringa oleifera leaf extracts inhibit 6β-hydroxylation of testosterone by CYP3A4. J. Infect. Dev. Ctries., 2(5):379-383.

[29]Nwidu, L.L., Cilli, E.M., Vilegas, W., 2012. Amino acid, antioxidant and ion profiles of Carpolobia lutea leaf (Polygalaceae). Tropical J. Pharm. Res., 11(5):807-813.

[30]Oktay, M., Gülçin, İ., Küfrevioğlu, Ö.İ., 2003. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT-Food Sci. Technol., 36(2):263-271.

[31]Orr, T.E., Mann, D.R., 1990. Effects of restraint stress on plasma LH and testosterone concentrations, Leydig cell LH/HCG receptors, and in vitro testicular steroidogenesis in adult rats. Horm. Behav., 24(3):324-341.

[32]Pourmorad, F., Hosseinimehr, S.J., Shahabimajd, N., 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr. J. Biotechnol., 5(11):1142-1145.

[33]Prabsattroo, T., Wattanathorn, J., Iamsa-ard, S., et al., 2012. Moringa oleifera leaves extract attenuates male sexual dysfunction. Am. J. Neurosci., 3(1):17-24.

[34]Purves-Tyson, T., Handelsman, D., Double, K., et al., 2012. Testosterone regulation of sex steroid-related mRNAs and dopamine-related mRNAs in adolescent male rat substantia nigra. BMC Neurosci., 13(1):95.

[35]Rai, J., Pandey, S.N., Srivastava, R.K., 2004. Testosterone hormone level in albino rats following restraint stress of long duration. J. Anat. Soc. India, 53(1):17-19.

[36]Retana-Márquez, S., Bonilla-Jaime, H., Vazquez-Palacios, G., et al., 2003. Changes in masculine sexual behavior, corticosterone and testosterone in response to acute and chronic stress in male rats. Horm. Behav., 44(4):327-337.

[37]Sanna, F., Succu, S., Hübner, H., et al., 2011. Dopamine D2-like receptor agonists induce penile erection in male rats: differential role of D2, D3 and D4 receptors in the paraventricular nucleus of the hypothalamus. Behav. Brain Res., 225(1):169-176.

[38]Santosh, B.T., Chitme, H.R., Rabbani, G., et al., 2011. Effect of Leptadenia reticulata Linn. on stress modulated sexual behavior of male rats. Int. Res. J. Pharm., 2(10):27-36.

[39]Schurr, A., Livne, A., 1976. Differential inhibition of mitochondrial monoamine oxidase from brain by hashish components. Biochem. Pharmacol., 25(10):1201-1203.

[40]Sreelatha, S., Padma, P.R., 2009. Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant Foods Hum. Nutr., 64(4):303-311.

[41]Thaipong, K., Boonprakob, U., Crosby, K., et al., 2006. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compost. Anal., 19(6-7):669-675.

[42]Xu, J.K., Hiroshi, K., Zheng, J.J., et al., 2006. Protective effect of tanshinones against liver injury in mice loaded with restraint stress. Acta Pharm. Sin., 41(7):631-635 (in Chinese).

[43]Xu, Y., Ku, B.S., Yao, H.Y., et al., 2005. The effects of curcumin on depressive-like behaviors in mice. Eur. J. Pharmacol., 518(1):40-46.

[44]Zaidi, S.K., Hoda, M.N., Tabrez, S., et al., 2014. Protective effect of Solanum nigrum leaves extract on immobilization stress induced changes in rat’s brain. Evid. Based Complement. Alternat. Med., 2014:912450.

[45]Zhang, Q., Radisavljevic, Z.M., Siroky, M.B., et al., 2011. Dietary antioxidants improve arteriogenic erectile dysfunction. Int. J. Androl., 34(3):225-235.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE