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CLC number: R542.2

On-line Access: 2018-05-05

Received: 2017-09-04

Revision Accepted: 2017-11-26

Crosschecked: 2018-04-18

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Citations:  Bibtex RefMan EndNote GB/T7714


Hui Zhou


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.5 P.333-341


Regulation of autophagy by tea polyphenols in diabetic cardiomyopathy

Author(s):  Hui Zhou, Yan Chen, Shu-wei Huang, Peng-fei Hu, Li-jiang Tang

Affiliation(s):  Department of Cardiology, Zhejiang Hospital, Hangzhou 310013, China; more

Corresponding email(s):   13757115395@163.com

Key Words:  Tea polyphenol, Autophagy, Diabetic cardiomyopathy, Obesity, Lipid metabolism disorder

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Hui Zhou, Yan Chen, Shu-wei Huang, Peng-fei Hu, Li-jiang Tang. Regulation of autophagy by tea polyphenols in diabetic cardiomyopathy[J]. Journal of Zhejiang University Science B, 2018, 19(5): 333-341.

@article{title="Regulation of autophagy by tea polyphenols in diabetic cardiomyopathy",
author="Hui Zhou, Yan Chen, Shu-wei Huang, Peng-fei Hu, Li-jiang Tang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Regulation of autophagy by tea polyphenols in diabetic cardiomyopathy
%A Hui Zhou
%A Yan Chen
%A Shu-wei Huang
%A Peng-fei Hu
%A Li-jiang Tang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 5
%P 333-341
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700415

T1 - Regulation of autophagy by tea polyphenols in diabetic cardiomyopathy
A1 - Hui Zhou
A1 - Yan Chen
A1 - Shu-wei Huang
A1 - Peng-fei Hu
A1 - Li-jiang Tang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 5
SP - 333
EP - 341
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700415

Objective: To investigate the effect of tea polyphenols on cardiac function in rats with diabetic cardiomyopathy, and the mechanism by which tea polyphenols regulate autophagy in diabetic cardiomyopathy. Methods: Sixty Sprague-Dawley (SD) rats were randomly divided into six groups: a normal control group (NC), an obesity group (OB), a diabetic cardiomyopathy group (DCM), a tea polyphenol group (TP), an obesity tea polyphenol treatment group (OB-TP), and a diabetic cardiomyopathy tea polyphenol treatment group (DCM-TP). After successful modeling, serum glucose, cholesterol, and triglyceride levels were determined; cardiac structure and function were inspected by ultrasonic cardiography; myocardial pathology was examined by staining with hematoxylin-eosin; transmission electron microscopy was used to observe the morphology and quantity of autophagosomes; and expression levels of autophagy-related proteins LC3-II, SQSTM1/p62, and Beclin-1 were determined by Western blotting. Results: Compared to the NC group, the OB group had normal blood glucose and a high level of blood lipids; both blood glucose and lipids were increased in the DCM group; ultrasonic cardiograms showed that the fraction shortening was reduced in the DCM group. However, these were improved significantly in the DCM-TP group. Hematoxylin-eosin staining showed disordered cardiomyocytes and hypertrophy in the DCM group; however, no differences were found among the remaining groups. Transmission electron microscopy revealed that the numbers of autophagosomes in the DCM and OB-TP groups were obviously increased compared to the NC and OB groups; the number of autophagosomes in the DCM-TP group was reduced. Western blotting showed that the expression of LC3-II/I and Beclin-1 increased obviously, whereas the expression of SQSTM1/p62 was decreased in the DCM and OB-TP groups (P<0.05). Conclusions: tea polyphenols had an effect on diabetic cardiomyopathy in rat cardiac function and may alter the levels of autophagy to improve glucose and lipid metabolism in diabetes.


方法:各组大鼠造模成功后测定血中的血糖、血脂水平;心超检测大鼠心脏结构及功能变化;苏木精-伊红(H&E)染色观察心肌结构及病理改变;透射电镜观察自噬体的形态和数量;蛋白质印迹法(Western blotting)检测自噬相关蛋白LC3-II、SQSTM1/p62及Beclin-1的表达水平.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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