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Received: 2019-07-28

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.2 P.172-177

http://doi.org/10.1631/jzus.B1900459


Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS


Author(s):  Yang Liu, You-Ran Shao, Xiang-Yu Li, Zhi-Ming Wang, Li-Rong Yang, Yu-Zhou Zhang, Mian-Bin Wu, Jian-Ming Yao

Affiliation(s):  Biotechnology Center, Institute of Plasma Physics and Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; more

Corresponding email(s):   wumb@zju.edu.cn, jmyao@ipp.ac.cn

Key Words:  Metabolism, Lycopene, Nicotine, Blakeslea trispora, GC-MS


Yang Liu, You-Ran Shao, Xiang-Yu Li, Zhi-Ming Wang, Li-Rong Yang, Yu-Zhou Zhang, Mian-Bin Wu, Jian-Ming Yao. Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS[J]. Journal of Zhejiang University Science B, 2020, 21(2): 172-177.

@article{title="Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS",
author="Yang Liu, You-Ran Shao, Xiang-Yu Li, Zhi-Ming Wang, Li-Rong Yang, Yu-Zhou Zhang, Mian-Bin Wu, Jian-Ming Yao",
journal="Journal of Zhejiang University Science B",
volume="21",
number="2",
pages="172-177",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900459"
}

%0 Journal Article
%T Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS
%A Yang Liu
%A You-Ran Shao
%A Xiang-Yu Li
%A Zhi-Ming Wang
%A Li-Rong Yang
%A Yu-Zhou Zhang
%A Mian-Bin Wu
%A Jian-Ming Yao
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 2
%P 172-177
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900459

TY - JOUR
T1 - Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS
A1 - Yang Liu
A1 - You-Ran Shao
A1 - Xiang-Yu Li
A1 - Zhi-Ming Wang
A1 - Li-Rong Yang
A1 - Yu-Zhou Zhang
A1 - Mian-Bin Wu
A1 - Jian-Ming Yao
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 2
SP - 172
EP - 177
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900459


Abstract: 
blakeslea trispora is a natural source of carotenoids, including β-carotene and lycopene, which have industrial applications. Therefore, classical selective breeding techniques have been applied to generate strains with increased productivity, and microencapsulated β-carotene preparation has been used in food industry (Li et al., 2019). In B. trispora, lycopene is synthesized via the mevalonate pathway (Venkateshwaran et al., 2015). lycopene cyclase, which is one of the key enzymes in this pathway, is a bifunctional enzyme that can catalyze the cyclization of lycopene to produce β-carotene and exhibit phytoene synthase activity (He et al., 2017).

在尼古丁诱导下的三孢布拉霉产番茄红素代谢的分析

概要:在三孢布拉霉发酵番茄红素过程中,阻断剂是必不可少的组分.尼古丁作为目前已知的最有效的番茄红素阻断剂之一,其在工业生产上具有巨大的实用价值.但阻断剂作为菌体的外源毒性生物碱,其对菌体的代谢势必有所改变.本文以尼古丁作为阻断剂,对其在三孢布拉霉发酵过程中的代谢影响做了初步研究.基于气相色谱-质谱(GC-MS)检测技术及小分子代谢组分的主成分分析(PCA),证明尼古丁在三孢布拉霉菌体内严重抑制氨基酸及糖代谢,同时脂肪酸和有机酸代谢也有所减弱,但相对强于糖代谢.因此,在尼古丁作为阻断剂时,为积累色素,碳源的补充应以脂肪酸或甘油为主.
关键词:代谢;番茄红素;尼古丁;三孢布拉霉;气相色谱-质谱

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Dihazi H, Kessler R, Eschrich K, 2004. High osmolarity glycerol (HOG) pathway-induced phosphorylation and activation of 6-phosphofructo-2-kinase are essential for glycerol accumulation and yeast cell proliferation under hyperosmotic stress. J Biol Chem, 279(23):23961-23968.

[2]Ding LJ, Chen JJ, Zou JD, et al., 2014. Dynamic metabolomic responses of Escherichia coli to nicotine stress. Can J Microbiol, 60(8):547-556.

[3]Fazeli MR, Tofighi H, Madadkar-Sobhani A, et al., 2009. Nicotine inhibition of lycopene cyclase enhances accumulation of carotenoid intermediates by Dunaliella salina CCAP 19/18. Eur J Phycol, 44(2):215-220.

[4]Fiehn O, Kopka J, Dörmann P, et al., 2000. Metabolite profiling for plant functional genomics. Nat Biotechnol, 18(11):1157-1161.

[5]Franzén CJ, 2003. Metabolic flux analysis of RQ-controlled microaerobic ethanol production by Saccharomyces cerevisiae. Yeast, 20(2):117-132.

[6]He ZJ, Wang SZ, Yang YM, et al., 2017. β-Carotene production promoted by ethylene in Blakeslea trispora and the mechanism involved in metabolic responses. Process Biochem, 57:57-63.

[7]Hohmann S, 2002. Osmotic stress signaling and osmoadaptation in yeasts. Microbiol Mol Biol Rev, 66(2):300-372.

[8]Jia HM, Li Q, Zhou C, et al., 2016. Chronic unpredictive mild stress leads to altered hepatic metabolic profile and gene expression. Sci Rep, 6(1):23441.

[9]Li XY, Wu MB, Xiao M, et al., 2019. Microencapsulated β-carotene preparation using different drying treatments. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 20(11):901-909.

[10]Liang MH, Hao YF, Li YM, et al., 2016. Inhibiting lycopene cyclases to accumulate lycopene in high β-carotene-accumulating Dunaliella bardawil. Food Bioprocess Technol, 9(6):1002-1009.

[11]Ma T, Shi B, Ye ZL, et al., 2019. Lipid engineering combined with systematic metabolic engineering of Saccharomyces cerevisiae for high-yield production of lycopene. Metab Eng, 52:134-142.

[12]Nissen TL, Schulze U, Nielsen J, et al., 1997. Flux distributions in anaerobic, glucose-limited continuous cultures of Saccharomyces cerevisiae. Microbiology, 143(1):203-218.

[13]Petelenz-Kurdziel E, Kuehn C, Nordlander B, et al., 2013. Quantitative analysis of glycerol accumulation, glycolysis and growth under hyper osmotic stress. PLoS Computat Biol, 9(6):e1003084.

[14]Roessner U, Luedemann A, Brust D, et al., 2001. Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell, 13(1):11-29.

[15]Sabir F, Loureiro-Dias MC, Soveral G, et al., 2017. Functional relevance of water and glycerol channels in Saccharomyces cerevisiae. FEMS Microbiol Lett, 364(9):fnx080.

[16]Seo EJ, Yeon YJ, Seo JH, et al., 2018. Enzyme/whole-cell biotransformation of plant oils, yeast derived oils, and microalgae fatty acid methyl esters into n-nonanoic acid, 9-hydroxynonanoic acid, and 1,9-nonanedioic acid. Bioresour Technol, 251:288-294.

[17]Singh G, Sinha S, Bandyopadhyay KK, et al., 2018. Triauxic growth of an oleaginous red yeast Rhodosporidium toruloides on waste ‘extract’ for enhanced and concomitant lipid and β-carotene production. Microb Cell Factor, 17(1):182.

[18]Şpaiuc D, Şpac AF, Agoroaei L, et al., 2014. Nicotine determination from tabacco by GC/MS. Farmacia, 62(5):983-990.

[19]Tereshina VM, Memorskaya AS, Feofilova EP, 2010. Lipid composition of the mucoraceous fungus Blakeslea trispora under lycopene formation-stimulating conditions. Microbiology, 79(1):34-39.

[20]Venkateshwaran M, Jayaraman D, Chabaud M, et al., 2015. A role for the mevalonate pathway in early plant symbiotic signaling. Proc Natl Acad Sci USA, 112(31):9781-9786.

[21]Villas-Bôas SG, Delicado DG, Åkesson M, et al., 2003. Simultaneous analysis of amino and nonamino organic acids as methyl chloroformate derivatives using gas chromatography-mass spectrometry. Anal Biochem, 322(1):134-138.

[22]Villas-Bôas SG, Moxley JF, Åkesson M, et al., 2005. High-throughput metabolic state analysis: the missing link in integrated functional genomics of yeasts. Biochem J, 388(2):669-677.

[23]Wagner C, Sefkow M, Kopka J, 2003. Construction and application of a mass spectral and retention time index database generated from plant GC/EI-TOF-MS metabolite profiles. Phytochemistry, 62(6):887-900.

[24]Zhang WJ, Liu C, Yang RJ, et al., 2019. Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 20(7):563-575.

[25]Zhou Y, Pijuan M, Zeng RJ, et al., 2009. Involvement of the TCA cycle in the anaerobic metabolism of polyphosphate accumulating organisms (PAOs). Water Res, 43(5):1330-1340.

[26]Zhuang XP, Zhang W, Zheng CX, et al., 2007. Effect of glucose, sodium glutamate and ethephon on synthetic quantity of lycopene in tomato fruits. J Anhui Agric Sci, 35(19):5664-5665, 5669 (in Chinese).

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