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CLC number: Q939.97

On-line Access: 2020-10-12

Received: 2020-06-01

Revision Accepted: 2020-08-18

Crosschecked: 2020-09-07

Cited: 0

Clicked: 1393

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Wen-na Bao

https://orcid.org/0000-0003-3452-7938

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.10 P.835-840

http://doi.org/10.1631/jzus.B2000269


Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid


Author(s):  Wen-na Bao, Yi Chen, Hong-xiu Liao, Hang Chen, Shi-wang Liu, Yong Liu

Affiliation(s):  School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China; more

Corresponding email(s):   wennabao@163.com

Key Words:  L(+)-Tartaric acid, cis-Epoxysuccinate hydrolase, Penicillium expansum, Isolation


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Wen-na Bao, Yi Chen, Hong-xiu Liao, Hang Chen, Shi-wang Liu, Yong Liu. Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid[J]. Journal of Zhejiang University Science B, 2020, 21(10): 835-840.

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author="Wen-na Bao, Yi Chen, Hong-xiu Liao, Hang Chen, Shi-wang Liu, Yong Liu",
journal="Journal of Zhejiang University Science B",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000269"
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A1 - Shi-wang Liu
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J0 - Journal of Zhejiang University Science B
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Abstract: 
The L(+)-form of tartaric acid (L(+)-TA) exists extensively in nature, and is widely used in the food, chemical, textile, building, and pharmaceutical industries (Su et al., 2001). The main method for L(+)-TA production is microbial transformation by cis-Epoxysuccinate hydrolase (CESH), which can catalyze the asymmetric hydrolysis of cis-epoxysuccinic acid or its salts to TA or tartrate (Bao et al., 2019). Seventeen species containing CESH have been isolated so far. However, most species for L(+)-TA production have been reported from bacteria (Xuan and Feng, 2019). The only fungus isolated from soil by our lab recently, that could be used as catalyst for the process under acidic condition, is Aspergillus niger WH-2 (Bao et al., 2020). In order to find strains with new characteristics, this study attempted to isolate a new CESH source from fungi and investigate its application value.

L(+)-酒石酸生产菌株扩展青霉WH-3的筛选

目的:筛选产L(+)-酒石酸的新真菌菌株.
创新点:首次筛选获得能同时在酸性和高底物浓度下转化生产L(+)-酒石酸的菌株.
方法:利用电镜、内转录间隔区(ITS)序列、核磁共振、质谱和旋光度对筛选菌株进行鉴定,并优化其发酵条件,研究其酶学性质.
结论:扩展青霉WH-3的60%活性位于菌丝体中,且该菌丝体能在酸性(pH 4.5)、高浓度底物中转化,具有高转化率和高立体特异性,为L(+)-酒石酸的生产提供新的催化剂.

关键词:L(+)-酒石酸;扩展青霉;顺式环氧琥珀酸水解酶;筛菌

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Bao WN, Luo ZS, Liu SW, et al., 2019. Cloning and characterization of an oxiranedicarboxylate hydrolase from Labrys sp. WH-1. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 20(12):995-1002.

[2]Bao WN, Liao HX, Chen Y, et al., 2020. Isolation of a novel strain Aspergillus niger WH-2 for production of L(+)-tartaric acid under acidic condition. Biotechnol Lett, 42(4):605-612.

[3]Cheng YQ, Pan HF, Bao WN, et al., 2014. Cloning, homology modeling, and reaction mechanism analysis of a novel cis-epoxysuccinate hydrolase from Klebsiella sp. Biotechnol Lett, 36(12):2537-2544.

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[11]List of electronic supplementary materials

[12]Fig. S1 Morphology of Penicillium expansum WH-3

[13]Fig. S2 1H-NMR (a), 13C-NMR (b), and mass spectrum (c) of the biotransformed product

[14]Materials and methods

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