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CLC number: Q939.97

On-line Access: 2020-10-12

Received: 2020-06-01

Revision Accepted: 2020-08-18

Crosschecked: 2020-09-07

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Wen-na Bao


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.10 P.835-840


Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid

Author(s):  Wen-na Bao, Yi Chen, Hong-xiu Liao, Hang Chen, Shi-wang Liu, Yong Liu

Affiliation(s):  School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China; more

Corresponding email(s):   wennabao@163.com

Key Words:  L(+)-Tartaric acid, cis-Epoxysuccinate hydrolase, Penicillium expansum, Isolation

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Wen-na Bao, Yi Chen, Hong-xiu Liao, Hang Chen, Shi-wang Liu, Yong Liu. Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid[J]. Journal of Zhejiang University Science B, 2020, 21(10): 835-840.

@article{title="Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid",
author="Wen-na Bao, Yi Chen, Hong-xiu Liao, Hang Chen, Shi-wang Liu, Yong Liu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid
%A Wen-na Bao
%A Yi Chen
%A Hong-xiu Liao
%A Hang Chen
%A Shi-wang Liu
%A Yong Liu
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 10
%P 835-840
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000269

T1 - Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid
A1 - Wen-na Bao
A1 - Yi Chen
A1 - Hong-xiu Liao
A1 - Hang Chen
A1 - Shi-wang Liu
A1 - Yong Liu
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 10
SP - 835
EP - 840
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000269

The L(+)-form of tartaric acid (L(+)-TA) exists extensively in nature, and is widely used in the food, chemical, textile, building, and pharmaceutical industries (Su et al., 2001). The main method for L(+)-TA production is microbial transformation by cis-Epoxysuccinate hydrolase (CESH), which can catalyze the asymmetric hydrolysis of cis-epoxysuccinic acid or its salts to TA or tartrate (Bao et al., 2019). Seventeen species containing CESH have been isolated so far. However, most species for L(+)-TA production have been reported from bacteria (Xuan and Feng, 2019). The only fungus isolated from soil by our lab recently, that could be used as catalyst for the process under acidic condition, is Aspergillus niger WH-2 (Bao et al., 2020). In order to find strains with new characteristics, this study attempted to isolate a new CESH source from fungi and investigate its application value.


结论:扩展青霉WH-3的60%活性位于菌丝体中,且该菌丝体能在酸性(pH 4.5)、高浓度底物中转化,具有高转化率和高立体特异性,为L(+)-酒石酸的生产提供新的催化剂.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[2]Bao WN, Liao HX, Chen Y, et al., 2020. Isolation of a novel strain Aspergillus niger WH-2 for production of L(+)-tartaric acid under acidic condition. Biotechnol Lett, 42(4):605-612.

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[8]Xu FX, Cao H, Cui XW, et al., 2018. Optimization of fermentation condition for echinacoside yield improvement with Penicillium sp. H1, an endophytic fungus isolated from Ligustrum lucidum Ait using response surface methodology. Molecules, 23(10):2586.

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[11]List of electronic supplementary materials

[12]Fig. S1 Morphology of Penicillium expansum WH-3

[13]Fig. S2 1H-NMR (a), 13C-NMR (b), and mass spectrum (c) of the biotransformed product

[14]Materials and methods

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