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Bio-Design and Manufacturing  2016 Vol.-1 No.-1 P.

http://doi.org/10.1631/bdm.2400313


Mg2+ containing composite scaffolds mediate macrophage polarization to enhance meniscus regeneration


Author(s):  Zong Li, Weili Shi, Ming Tian, Wenqiang Yan, Yifei Fan, Jin Cheng, Xiaoqing Hu, Xi Gong, Yingfang Ao

Affiliation(s):  Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing, China; more

Corresponding email(s):   Gongxibjmu@sina.com, aoyingfang@163.com

Key Words:  meniscus repair, magnesium, macrophage polarization, multifunctional composite hydrogel


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Abstract: 
Meniscal injury, a prevalent and challenging medical condition, is characterized by poor self-healing potential and a complex microenvironment. Tissue engineering scaffolds, particularly those made of silk fibroin (SF)/hyaluronic acid methacryloy (HAMA) and encapsulating Mg2+, are promising options for meniscal repair. However, the inflammatory response following implantation is a significant concern. In this study, we prepared a composite SF/HAMA-Mg hydrogel scaffold, evaluated its physical and chemical properties, and detected its fibrochondrogenic differentiation effect in vitro and the healing effect in a rabbit meniscus defect model in vivo. Our results showed that the scaffold differentiates pro-inflammatory M1 macrophages into anti-inflammatory M2 macrophages after implantation, thereby reducing inflammation and facilitating the growth and repair of meniscus tissue. Further, the composite scaffold provided a conducive milieu for cell proliferation, anticipatory differentiation, and generation of extracellular matrix. In summary, composite SF/HAMA-Mg scaffolds display exceptional biocompatibility, anti-inflammatory properties, and superior potential for meniscus repair.

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