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Abstract: Mesenchymal stem/stromal cell (MSC)‍-based therapy has been regarded as one of the most revolutionary breakthroughs in the history of modern medicine owing to its myriad of immunoregulatory and regenerative properties. With the rapid progress in the fields of osteo- and musculoskeletal therapies, the demand for MSC-based treatment modalities is becoming increasingly prominent. In this endeavor, researchers around the world have devised new and innovative techniques to support the proliferation of MSCs while minimizing the loss of hallmark features of stem cells. One such example is electromagnetic field (EMF) exposure, which is an alternative approach with promising potential. In this review, we present a critical discourse on the efficiency, practicability, and limitations of some of the relevant methods, with insurmountable evidence backing the implementation of EMF as a feasible strategy for the clinically relevant expansion of MSCs.

电磁场暴露作为在临床相关情况下增强间充质干细胞增殖和分化的一种可行方法

概述：在这篇综述中，我们对一些相关方法的效率、实用性和局限性进行了批判性讨论，并支持将电磁场（EMF）作为临床相关间充质干细胞（MSCs）扩展的可行策略。本文旨在寻找促进MSCs增殖和分化而没有细胞毒性和基因毒性作用的新疗法，可以为再生医学和组织工程领域带来新的思路。随着最近细胞培养方法的进步和专用生物反应器的出现，EMF疗法显然是进一步促进MSCs增殖和分化的一种有前途的方法。在过去，EMF疗法已被证实是一种成功用作治疗骨病的有效且非侵入性的方法。同时，各种研究结果证明了0.2-5.0 mT和15-75 Hz的EMF对不同治疗时间的MSCs增殖和分化的积极影响。尽管EMF暴露作为MSCs临床扩展的新策略已显示出巨大潜力，但有必要进一步检查EMF的核心特性，以确定在增强MSCs增殖的同时保持其分化潜能和干细胞的独特暴露范围。

关键词：电磁场；增殖；间充质干细胞；治疗

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